A5056618662- 3D Printing in Biomedical Research
- Electrospun Nanofibers in Biomedical Applications
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Tissue Engineering and Regenerative Medicine
- Circular RNAs in diseases
- Additive Manufacturing and 3D Printing Technologies
- Angiogenesis and VEGF in Cancer
- RNA modifications and cancer
- Bone Tissue Engineering Materials
- Barrier Structure and Function Studies
- Reconstructive Surgery and Microvascular Techniques
- Mesenchymal stem cell research
- Pluripotent Stem Cells Research
- Cell Adhesion Molecules Research
- Intestinal and Peritoneal Adhesions
- Pancreatic function and diabetes
- Wound Healing and Treatments
- Breast Implant and Reconstruction
- Biomedical Ethics and Regulation
- Hernia repair and management
- Nerve injury and regeneration
- Diabetes Management and Research
- Peripheral Artery Disease Management
- Monoclonal and Polyclonal Antibodies Research
Penn State Milton S. Hershey Medical Center
2016-2025
Pennsylvania State University
2006-2025
Creative Commons
2017-2023
University of Pittsburgh Medical Center
2018
University of Pittsburgh
2018
Hershey (United States)
2017
Brigham and Women's Hospital
2005-2010
Indiana University School of Medicine
2010
Indiana University – Purdue University Indianapolis
2010
Harvard University
2005-2009
: Three-dimensional (3D) bioprinting is a revolutionary technology in building living tissues and organs with precise anatomic control cellular composition. Despite the great progress research, there has yet to be any clinical translation due current limitations human-scale constructs, which are vascularized readily implantable. In this article, we review challenges 3D bioprinting, including situ techniques, one of several translational models facilitate application from bench bedside. A...
Abstract Reconstruction of complex craniomaxillofacial (CMF) defects is challenging due to the highly organized layering multiple tissue types. Such compartmentalization necessitates precise and effective use cells other biologics recapitulate native anatomy. In this study, intra‐operative bioprinting (IOB) different CMF tissues, including bone, skin, composite (hard/soft) demonstrated directly on rats in a surgical setting. A novel extrudable osteogenic hard ink introduced, which induced...
Abstract Bulk hydrogel scaffolds are common in reconstructive surgery. They allow for the staged repair of soft tissue loss by providing a base revascularization. Unfortunately, they limited both slow and random vascularization, which may manifest as treatment failure or suboptimal repair. Rapidly inducing patterned vascularization within biomaterials has profound translational implications current clinical paradigms scaleup regenerative engineering platforms. To address this long‐standing...
The heterogeneous and anisotropic articular cartilage is generally studied as a layered structure of "zones" with unique composition architecture, which difficult to recapitulate using current approaches. A novel hybrid bioprinting strategy presented here generate zonally stratified cartilage. Scaffold-free tissue strands (TSs) are made human adipose-derived stem cells (ADSCs) or predifferentiated ADSCs. Cartilage TSs ADSCs exhibit improved mechanical properties upregulated expression...
Abstract Granular hydrogel scaffolds (GHS) are fabricated via placing microparticles (HMP) in close contact (packing), followed by physical and/or chemical interparticle bond formation. Gelatin methacryloyl (GelMA) GHS have recently emerged as a promising platform for biomedical applications; however, little is known about how the packing of building blocks, physically crosslinked soft GelMA HMP, affects (pore microarchitecture and mechanical/rheological properties) biological (in vitro...
An ultrasound-responsive hydrogel system was developed that provides on-demand release when stimulated by focused ultrasound (fUS). Diels–Alder cycloadducts crosslinked polyethylene glycol (PEG) hydrogels and underwent a retrograde reaction exposed to fUS. Four-arm eight-arm furan-based compositions were used evaluate the link between crosslinking density fUS-induced retention rates. PEG with glutaraldehyde also as non-Diels–Alder control hydrogel. By increasing exposure time amplitude of...
Intussusceptive angiogenesis is a morphogenetic process that forms new blood vessels by the division of single vessel into two lumens. Here, we show this intraluminal participates in inflammation-induced neovascularization associated with chemically induced murine colitis. In studies both acute (4-7 days) and chronic (28-31 colitis, intravital microscopy intravascular tracers demonstrated twofold reduction flow velocity. colitis model, decreased velocity was marked dilatation mucosal plexus....
Cancer is the second leading cause of death in United States and a major public health concern worldwide.Basic, clinical epidemiological research to improved cancer detection, prevention, outcomes.Recent technological advances have allowed unbiased comprehensive screening genome-wide gene expression.Small non-coding RNAs (sncRNAs) been shown play an important role biological processes could serve as diagnostic, prognostic therapeutic biomarker for specific diseases.Recent findings begun...
Abstract Hepatocellular carcinoma (HCC) was the fifth leading cause of cancer death in men and eighth women United States 2017. In our study, we sought to identify sncRNAs various stages development HCC. We obtained publicly available small RNA-seq data derived from patients with cirrhosis (n = 14), low-grade dysplastic nodules (LGDN, n 9), high grade (HGDN, 6), early hepatocellular (eHCC, advanced (HCC, 20), along healthy liver tissue samples 9). All were analyzed for types non-coding RNAs...
Abstract Increasing occurrences of degenerative diseases, defective tissues, and severe cancers heighten the importance advanced biomedical treatments, which in turn enhance need for improved biomaterials with versatile theranostic functionalities yet using minimal design complexity. Leveraging advantages citrate chemistry, a multifunctional citrate‐based biomaterial platform is developed both imaging therapeutic capabilities utilizing facile efficient one‐pot synthesis. The resulting...
Introduction Ongoing recognition of breast implant–associated anaplastic large cell lymphoma (BIA-ALCL) and its link with textured devices has brought a paradigm shift in prosthetic-based reconstruction. Many institutions no longer offer expansion for staged However, there is paucity data regarding the efficacy smooth tissue expanders (TE). We hypothesized that time to final reconstruction complication profile between TEs would be similar patients. Methods A retrospective chart review was...
Improved healthcare and recent breakthroughs in technology have substantially reduced cancer mortality rates worldwide. Recent advancements next-generation sequencing (NGS) allowed genomic analysis of the human transcriptome. Now, using NGS we can further look into small non-coding regions RNAs (sncRNAs) such as microRNAs (miRNAs), Piwi-interacting-RNAs (piRNAs), long (lncRNAs), nuclear/nucleolar (sn/snoRNAs) among others. studies looking at sncRNAs indicate their role important biological...
Craniomaxillofacial (CMF) reconstruction is a challenging clinical dilemma. It often necessitates skin replacement in the form of autologous graft or flap surgery, which differ from one another based on hypodermal/dermal content. Unfortunately, both approaches are plagued by scarring, poor cosmesis, inadequate restoration native anatomy and hair, alopecia, donor site morbidity, potential for failure. Therefore, new reconstructive warranted, tissue engineered represents an exciting...
Abstract Hydrogels are promising scaffolds for adipose tissue regeneration. Currently, the incorporation of bioactive molecules in hydrogel system is used, which can increase cell proliferation rate or improve adipogenic differentiation performance stromal stem cells but often suffers from high expense cytotoxicity because light/thermal curing used polymerization. In this study, decellularized incorporated, at varying concentrations, with a thiol‐acrylate fraction that then polymerized to...
The scalability of cell aggregates such as spheroids, strands, and rings has been restricted by diffusion nutrient oxygen into their core. In this study, we introduce a novel concept in generating tissue building blocks with micropores, which represents an alternative solution for vascularization. Sodium alginate porogens were mixed human adipose-derived stem cells, loaded tubular capsules, followed de-crosslinking the capsules. resultant cellular structure exhibited porous morphology formed...
Blood flowing across the vascular endothelium creates wall shear stress, dependent on velocity of flow and vessel geometry, that tends to disrupt lymphocyte-endothelial cell adhesion.The microcirculation in a murine model acute colitis was investigated identify structural adaptations during may facilitate transmigration.In 2,4,6-trinitrobenzenesulphonic acid-induced colitis, infiltrating cells colonic by cellular topographic mapping, corrosion casting three-dimensional scanning electron...