A5022636715- 3D Printing in Biomedical Research
- Additive Manufacturing and 3D Printing Technologies
- Innovative Microfluidic and Catalytic Techniques Innovation
- Cancer Cells and Metastasis
- Lysosomal Storage Disorders Research
- Cell Image Analysis Techniques
- CAR-T cell therapy research
- Bone Tissue Engineering Materials
- Gut microbiota and health
- Mesenchymal stem cell research
- Neonatal Respiratory Health Research
- Clostridium difficile and Clostridium perfringens research
- Tissue Engineering and Regenerative Medicine
- Nanoplatforms for cancer theranostics
- Nanoparticle-Based Drug Delivery
- Osteoarthritis Treatment and Mechanisms
- Respiratory viral infections research
- Respiratory Support and Mechanisms
- biodegradable polymer synthesis and properties
- Gastrointestinal motility and disorders
- Pluripotent Stem Cells Research
- Metal-Organic Frameworks: Synthesis and Applications
Johns Hopkins University
2025
Pennsylvania State University
2020-2024
Millennium Engineering and Integration (United States)
2023-2024
Park University
2023
Abstract Bioprinting of cellular aggregates, such as tissue spheroids, to form three-dimensional (3D) complex-shaped arrangements, has posed a major challenge due lack robust, reproducible and practical bioprinting techniques. Here, we demonstrate 3D aspiration-assisted freeform spheroids by precisely positioning them in self-healing yield-stress gels, enabling the self-assembly for fabrication tissues. The presented approach enables traverse directly from cell media gel on demand. We study...
Aspiration-assisted freeform bioprinting (AAfB) has emerged as a promising technique for precise placement of tissue spheroids in three-dimensional (3D) space enabling fabrication. To achieve success embedded using AAfB, an ideal support bath should possess shear-thinning behavior and yield-stress to facilitate tight fusion assembly bioprinted forming tissues. Several studies have demonstrated baths the past few years, yet majority these materials poses challenges due their low...
Abstract Current nucleic acid delivery methods have not achieved efficient, non‐toxic of miRNAs with tumor‐specific selectivity. In this study, a new system based on light‐inducible gold–silver–gold, core–shell–shell (CSS) nanoparticles is presented. This delivers small therapeutics precise spatiotemporal control, demonstrating the potential for achieving selectivity and efficient miRNA mimics. The particles leverage photothermal heating metal due to local surface plasmonic resonance...
Despite substantial advancements in development of cancer treatments, lack standardized and physiologically-relevant vitro testing platforms limit the early screening anticancer agents. A major barrier is complex interplay between tumor microenvironment immune response. To tackle this, a dynamic-flow based 3D bioprinted multi-scale vascularized breast model, responding to chemo immunotherapeutics developed. Heterotypic tumors are precisely at pre-defined distances from perfused vasculature,...
Abstract The gut microbiota influences systemic immunity and the function of distal tissues, including brain, liver, skin, lung, muscle. However, role in foreign body response (FBR) fibrosis around medical implants is largely unexplored. To investigate this connection, we perturbed homeostasis murine via enterotoxigenic Bacteroides fragilis (ETBF) infection implanted synthetic polymer polycaprolactone (PCL) into a muscle injury. ETBF mice led to increased neutrophil γδ T cell infiltration...
Abstract The engineering of osteochondral interfaces remains a challenge. MicroRNAs (miRs) have emerged as significant tools to regulate the differentiation and proliferation osteogenic chondrogenic formation in human musculoskeletal system. Here, we describe novel approach reconstruction based on three-dimensional (3D) bioprinting miR-transfected adipose-derived stem cell (ADSC) spheroids produce heterotypic interface that addresses intrinsic limitations traditional inducing zonal via use...
Tissue biofabrication that replicates an organ-specific architecture and function requires physiologically-relevant cell densities. Bioprinting using spheroids has the potential to create constructs with native densities, but its application is limited due lack of practical, scalable techniques. This study presents HITS-Bio (High-throughput Integrated Fabrication System for Bioprinting), a novel multiarray spheroid bioprinting technology enabling tissue fabrication by rapidly positioning...
Tissue biofabrication mimicking organ-specific architecture and function requires physiologically-relevant cell densities. Bioprinting using spheroids can achieve this, but is limited due to the lack of practical, scalable techniques. This study presents HITS-Bio (High-throughput Integrated Fabrication System for Bioprinting), a multiarray bioprinting technique rapidly positioning multiple simultaneously digitally-controlled nozzle array (DCNA). achieves an unprecedented speed, ten times...
Engineered bone grafts require a vascular network to supply cells with oxygen, nutrients and remove waste. Using heterotypic mature create these in vivo has resulted limited cell density, ectopic tissue formation disorganized tissue. Despite evidence that progenitor aggregates, such as spheroids, are potential candidate for fabrication of native-like pre-vascularized tissue, the factors dictating co-differentiation remains poorly understood. In this study, we examined three-dimensional...
Gene therapeutic applications combined with bio- and nano-materials have been used to address current shortcomings in bone tissue engineering due their feasibility, safety potential capability for clinical translation. Delivery of non-viral vectors can be altered using gene-activated matrices improve efficacy repair defects.Ex-situandin-situdelivery strategies are the most methods therapy, which never directly compared potency critical-sized defects. In this regard, we first time explore...
Gaucher disease (GD), the most prevalent lysosomal disorder, is caused byGBA1gene mutations, leading to deficiency of glucocerebrosidase, and accumulation glycosphingolipids in cells mononuclear phagocyte system. While skeletal diseases are cause morbidity reduced quality life GD, pathophysiology bone involvement not yet fully understood, partly due lack relevant human model systems. In this work, we present first 3D GD using aspiration-assisted freeform bioprinting, which enables a platform...
Engineering functional tissues and organs remains a fundamental pursuit in bio-fabrication. However, the accurate constitution of complex shapes internal anatomical features specific organs, including their intricate blood vessels nerves, significant challenge. Inspired by Matryoshka doll, here new method called "Intra-Embedded Bioprinting (IEB)" is introduced building upon existing embedded bioprinting methods. xanthan gum-based material used which served dual role as both bioprintable ink...
Cell aggregates are widely used to study heterotypic cellular interactions during the development of vascularization
Abstract Bioprinting of cellular aggregates, such as tissue spheroids or organoids, in complex three-dimensional (3D) arrangements has been a major obstacle for scaffold-free fabrication tissues and organs. In this research, we unveiled new approach to the bioprinting yield stress granular gel, which exhibited unprecedented capabilities freeform positioning 3D. Due its Herschel-Bulkley self-healing properties well biological inertness, gel supported both self-assembly spheroids. We studied...
Abstract In this study, we developed a three-dimensionally (3D) printed lung model that faithfully recapitulates the intricate environment. This 3D incorporated alveolar and vascular components allow for comprehensive exploration of physiology responses to infection in vitro . particular, investigated role ventilation on formation epithelial layer its response viral infections. regard, subjected our printed, perfused continuous respiratory cycle at air-liquid interface (ALI) up 10 days...
Engineering functional tissues and organs remains a fundamental pursuit in biofabrication. However, the accurate constitution of complex shapes internal anatomical features specific organs, including their intricate blood vessels nerves, significant challenge. Inspired by Matryoshka doll, we here introduce new method called 'Intra-Embedded Bioprinting (IEB),' building upon existing embedded bioprinting methods. We used xanthan gum-based material, which served dual role as both bioprintable...
Abstract Aspiration-assisted freeform bioprinting (AAfB) has emerged as a promising technique for precise placement of tissue spheroids in three-dimensional (3D) space fabrication tissues. For successful embedded using AAfB, an ideal support bath should possess shear-thinning behavior and yield-stress to obtain tightly fused assembly bioprinted spheroids. Several studies have demonstrated baths bioprinting, but these materials pose major challenges due their low biocompatibility, opaqueness,...