A5069263424- Animal Nutrition and Physiology
- Antimicrobial Peptides and Activities
- Protein Hydrolysis and Bioactive Peptides
- Biochemical and Structural Characterization
- Proteins in Food Systems
- Animal Genetics and Reproduction
- Protease and Inhibitor Mechanisms
- Blood Coagulation and Thrombosis Mechanisms
- Plant Reproductive Biology
- Sperm and Testicular Function
- Phytase and its Applications
- Livestock and Poultry Management
- S100 Proteins and Annexins
- Antioxidant Activity and Oxidative Stress
- Invertebrate Immune Response Mechanisms
- Cell Adhesion Molecules Research
- Peptidase Inhibition and Analysis
- Enzyme Production and Characterization
- Coccidia and coccidiosis research
- Agriculture and Rural Development Research
- Neurobiology and Insect Physiology Research
- Food and Agricultural Sciences
- Biotin and Related Studies
- Immune Response and Inflammation
- Identification and Quantification in Food
Biologie des Oiseaux et Aviculture
2013-2024
Université de Tours
2005-2022
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2013-2022
Centre Val de Loire
2018
Inserm
2005-2014
Physiologie de la Reproduction et des Comportements
2005-2013
Centre Hospitalier Universitaire de Reims
2009-2012
Université de Reims Champagne-Ardenne
2009-2012
Hôpital Maison Blanche
2009-2012
Fondation de l'Avenir
2012
Cigarette smoking is a major factor for the development of pulmonary emphysema because it induces abnormal inflammation and protease-rich local milieu that causes connective tissue breakdown lungs. As result its capacity to degrade lung high risk patients lacking α1-antitrypsin develop emphysema, much interest has focused on neutrophil elastase (NE). Two similar serine proteases (NSPs), cathepsin G proteinase 3, coexist with NE in humans mice, but their potential tissue-destructive role(s)...
Elafin is a neutrophil serine protease inhibitor expressed in lung and displaying anti-inflammatory anti-bacterial properties. Previous studies demonstrated that some innate host defense molecules of the cystic fibrosis (CF) chronic obstructive pulmonary disease airways are impaired due to increased proteolytic degradation observed during inflammation. In light these findings, we thus focused on status elafin CF lung. We showed present study cleaved sputum from individuals with CF....
There is accumulating evidence that following bacterial infection, the massive recruitment and activation of phagocytes, neutrophils, accompanied with extracellular release active neutrophil elastase (NE), a potent serine protease. Using NE-deficient mice in clinically relevant model Pseudomonas aeruginosa-induced pneumonia, we provide compelling vivo absence NE was associated decreased protein transcript levels proinflammatory cytokines TNF-α, MIP-2, IL-6 lungs, coinciding increased...
Abstract The chicken egg resists most environmental microbes suggesting that it potentially contains efficient antimicrobial molecules. Considering some heparin-binding proteins in mammals are antibacterial, we investigated the presence and activity of from white. Mass spectrometry analysis recovered after heparin-affinity chromatography, revealed 20 proteins, including known (avidin, lysozyme, TENP, ovalbumin-related protein X avian bêta-defensin 11). antibacterial three new candidates...
Abstract Background Egg defence against bacterial contamination relies on immunoglobulins (IgY) concentrated in the yolk and antimicrobial peptides/proteins predominantly localized egg white (EW). Hens contaminated with pathogenic microorganisms export specific IgYs to (adaptative immunity). No evidence of such regulation has been reported for (innate immunity) which are preventively secreted by hen oviduct active a large range microbes. We investigated whether innate defences can be...
Egg white is an important contributor to the protection of eggs against bacterial contaminations during first half incubation (day zero 12), prior egg transfer into amniotic fluid be orally absorbed by embryo. This protective system relies on arsenal antimicrobial proteins and intrinsic physicochemical properties that are generally unfavorable for multiplication dissemination. Some changes in these parameters can observed storage incubation. The aim this work was characterize antibacterial...
Elafin and its precursor trappin-2 (also called pre-elafin) are potent protein inhibitors of neutrophil serine proteases such as leukocyte elastase proteinase 3. Trappin-2 has unique conserved sequence motifs rich in Gln Lys residues. These substrates for transglutaminases that may enable to be cross-linked extracellular matrix proteins, thus anchoring the inhibitor at site action. We have used Western blotting ELISA-based assays demonstrate both elafin can conjugated various proteins vitro...
It is widely accepted that neutrophil serine proteases (NSPs) play a critical role in neutrophil-associated lung inflammatory and tissue-destructive diseases. To investigate NSP pathogenic role(s), various mouse experimental models have been developed mimic acutely or chronically injured human lungs. We others are using exposure to cigarette smoke as model for chronic obstructive pulmonary disease with without exacerbation. However, the relative contribution of NSPs processes well their...
In many amniotes, the amniotic fluid is depicted as a dynamic milieu that participates in protection of embryo (cushioning, hydration, and immunity). However, birds, protein profile remains unexplored, even though its proteomic signature predicted to differ compared with humans. fact, unlike humans, chicken does not collect excretory products composition strikingly changes at mid-development because massive inflow egg white proteins, which are thereafter swallowed by support growth. Using...
The ovalbumin gene family in Gallus gallus is composed of three homologous genes located within a 46 kb locus on chromosome 2: ovalbumin, ovalbumin-related protein Y (OVAY), and X (OVAX) genes. expression these hen oviduct under estrogen control, but their relative hormonal responsiveness subsequent concentration egg, distinctive. Interestingly, all proteins lack the classical signal peptide for secretion. Ovalbumin, OVAX, OVAY belong to serine protease inhibitor (serpin) whose members share...
A number of serine, cysteine, metallo- and acid proteases were evaluated for their ability to proteolytically cleave the serine protease inhibitor trappin-2, also known as pre-elafin, release elafin from its precursor. None metalloproteases or examined cleaved while cysteine preferentially trappin-2 within non-inhibitory N-terminal moiety. Cathepsin L, cathepsin K, plasmin, trypsin tryptase able by cleaving Lys38-Ala39 peptide bond in trappin-2. However, purified appeared be efficient at...
The secretory leukocyte protease inhibitor (SLPI), elafin, and its biologically active precursor trappin-2 are endogeneous low-molecular weight inhibitors of the chelonianin family that control enzymatic activity neutrophil serine proteases (NSPs) like elastase, proteinase 3, cathepsin G. These may be therapeutic value, since unregulated NSP activities linked to inflammatory lung diseases. However SLPI inhibits elastase G but not while elafin targets 3 We have used two strategies design...
Abstract Elafin is a 6-kDa innate immune protein present at several epithelial surfaces including the pulmonary epithelium. It canonical protease inhibitor of two neutrophil serine proteases [neutrophil elastase (NE) and proteinase 3] with capacity to covalently bind extracellular matrix proteins by transglutamination. In addition these properties, elafin also possesses antimicrobial immunomodulatory activities. The aim study was investigate effect Pseudomonas aeruginosa on function. We...
Out of the 14 avian β-defensins identified in Gallus gallus genome, only 3 are present chicken egg, including egg-specific β-defensin 11 ( Gga -AvBD11). Given its specific localization and established antibacterial activity, -AvBD11 appears to play a protective role embryonic development. is an atypical double-sized defensin, predicted possess 2 motifs related 6 disulfide bridges. The 3-dimensional NMR structure purified Gga- AvBD11 compact fold composed packed domains. This archetype...
The avian egg perivitelline layer (PL) is a proteinaceous structure that encloses the yolk. It consists of inner and outer layers (IPL OPL, respectively) are assumed to play distinct roles in bird reproduction. To gain insight into their respective function, we analyzed proteome IPL OPL chicken unfertilized eggs after mechanical separation, using GeLC-MS/MS strategy. Of 412 proteins identified, 173 were uniquely recovered 98 while 141 identified both sublayers. Genes coding most abundant...