A5007706991- Connexins and lens biology
- Neuroscience and Neuropharmacology Research
- Nicotinic Acetylcholine Receptors Study
- Cannabis and Cannabinoid Research
- RNA regulation and disease
- Ion channel regulation and function
- Mass Spectrometry Techniques and Applications
- Natural Compound Pharmacology Studies
- Neuroscience of respiration and sleep
- Biochemical effects in animals
- Adenosine and Purinergic Signaling
University of Calgary
2017-2024
Allen Institute for Brain Science
2024
Key points The large‐pore channel pannexin 1 (Panx1) is expressed in many cell types and can open upon different, yet not fully established, stimuli. Panx1 permeability often inferred from to fluorescent dyes, but it currently unknown whether dye translates other molecules. Cell shrinkage C‐terminal cleavage led a open‐state with increased atomic ions (current), did alter ethidium uptake. inhibitors affected Panx1‐mediated ion conduction differently permeability, inhibitor efficiency towards...
Abstract Opioid withdrawal is a liability of chronic opioid use and misuse, impacting people who prescription or illicit opioids. Hyperactive autonomic output underlies many the aversive symptoms that make it difficult to discontinue use. The locus coeruleus (LC) an important centre within brain with poorly defined role in withdrawal. We show here pannexin-1 (Panx1) channels expressed on microglia critically modulate LC activity during Within LC, we found spinally projecting tyrosine...
Abstract In recent years, evidence supporting non‐ionotropic signalling by the NMDA receptor (niNMDAR) has emerged, including roles in long‐term depression (LTD). Here, we investigated whether niNMDAR‐pannexin‐1 (Panx1) contributes to LTD at CA3–CA1 hippocampal synapse. Using whole‐cell, patch clamp electrophysiology rat slices, show that a low‐frequency stimulation (3 Hz) of Schaffer collaterals produces is blocked continuous but not transient application NMDAR competitive antagonist,...
Abstract Anandamide is an endovanilloid and endocannabinoid with ligand activity at transient receptor potential vanilloid 1 channels cannabinoid receptors, respectively. We have reported that block of Pannexin-1 in the CA1 hippocampus can increase concentrations anandamide induce presynaptic plasticity. It not known how ion channel contribute to clearance a lipid-derived signalling molecule. Here, we use electrophysiology imaging uptake fluorescent determine structure-function relationship...