A5062779567- Ocular and Laser Science Research
- Advanced Fluorescence Microscopy Techniques
- Visual perception and processing mechanisms
- Nonlinear Optical Materials Studies
- Advanced Fiber Optic Sensors
- Retinal Diseases and Treatments
- Photonic and Optical Devices
- Optical Coherence Tomography Applications
- Ophthalmology and Visual Impairment Studies
- Semiconductor Lasers and Optical Devices
- Intraocular Surgery and Lenses
- Advanced Optical Sensing Technologies
- Digital Holography and Microscopy
- Spectroscopy Techniques in Biomedical and Chemical Research
- Random lasers and scattering media
- Retinal and Optic Conditions
- Extracellular vesicles in disease
- Optical Network Technologies
- Microfluidic and Bio-sensing Technologies
- Optical Polarization and Ellipsometry
- Autoimmune Bullous Skin Diseases
- Optical measurement and interference techniques
- Photoacoustic and Ultrasonic Imaging
- Color Science and Applications
- Analytical Chemistry and Sensors
Warsaw University of Technology
2024
Polish Academy of Sciences
2018-2023
Gdańsk University of Technology
2016-2023
Institute of Physical Chemistry
2019-2022
International Institute for Molecular Oncology
2021-2022
Abstract In this contribution a novel polarization gratings aided common‐path Hilbert holotomography (CP‐HHT) is presented for label‐free 3D refractive index imaging. Addressing limitations in current methods, the extended space‐bandwidth product leveraged through robust phase demodulation using spiral transform. The application of diffraction system enables fully tailored holographic settings such as fringe density and shear, allowing flexible hologram demodulation, while maintaining...
BackgroundNoninvasive assessment of metabolic processes that sustain regeneration human retinal visual pigments (visual cycle) is essential to improve ophthalmic diagnostics and accelerate development new treatments counter diseases. Fluorescent vitamin A derivatives, which are the chemical intermediates these processes, highly sensitive UV light; thus, safe analyses in humans currently beyond reach even most modern ocular imaging modalities.MethodsWe present a compact, 2-photon-excited...
This report presents the results of measurements two-photon vision threshold for various pulse trains. We employed three pulsed near-infrared lasers and stretchers to obtain variations duty cycle parameter over orders magnitude. proposed extensively described a mathematical model that combines laser parameters with visual value. The presented methodology enables one predict value stimulus healthy subject while using source known parameters. Our findings would be engineers community...
Two-photon vision is a phenomenon associated with the perception of short pulses near-infrared radiation (900-1200 nm) as visible light. It caused by nonlinear process two-photon absorption visual pigments. Here we present results showing influence pulse duration and repetition rate pulsed lasers on threshold. We compared sensitivity maps retina obtained for subjects normal using cost-effective fiber laser ( λ c = 1028.4 nm, τ p 12.2 ps, F rep 19.17 MHz) solid-state 1043.3 0.253 62.65 MHz)....
A fair comparison of multiple live cell cultures requires examining them under identical environmental conditions, which can only be done accurately if all cells are prepared simultaneously and studied at the same time place. This contribution introduces a multiplexed lensless digital holographic microscopy system (MLS), enabling synchronous, label-free, quantitative observation with single-cell precision. The innovation this setup lies in its ability to robustly compare behaviour, i.e.,...
We report the development of a widely-tunable femtosecond fiber laser system and its application for two-photon vision studies. The source is based on an Er-doped with spectral shift up to 2150 nm, followed by second harmonic generation module generate frequency-doubled beam tunable from 872 1075 nm. delivers sub-230 fs pulses nearly-constant duration over entire tuning range, output powers between 0.68-1.24 mW, which corresponds pulse energy 13.2-24.1 pJ. Such sufficient employing...
Distributed measurement often relies on sensor networks.In this paper, we present the construction of low-coherence fiber-optic Fabry-Pérot sensors connected into a quasi-distributed network.We discuss mechanism spectrum modulation in type and constraints assembly such network.Particular attention was paid to separate signals from individual which can be achieved by cavity length-based addressing.We designed built laboratory model temperature network.The employed are interferometric...
Abstract In this paper, a spectroscopic method for determination of cyclosporine concentrations in biological fluids is presented. Blood plasma and hemoglobin solutions are chosen the experiment. For various blood hemoglobin, absorbance measurements spectra range from 600 to 1100 nm performed. The measurement results analyzed by use dedicated algorithm. obtained data characterized high coefficient correlation R 2 , which equal 0.9461 0.9808 respectively. proposed enables selective detection...
Pulsed near-infrared (NIR) light sources can be successfully applied for both imaging and functional testing of the human eye, as published recently. These two groups applications have different requirements. For applications, most preferable is invisible scanning beam while efficiently visible stimulating applications. The eye using NIR laser beams possible due to two-photon vision (2PV) phenomenon. 2PV enables perception pulsed color corresponding approximately half wavelength. This study...
This research presents the WUTScope, a novel interferometric microscope developed by Quantitative Computational Imaging group at Warsaw University of Technology. system, leveraging Phase Microscopy and Optical Diffraction Tomography, provides insightful three-dimensional reconstructions refractive index distribution in semi-transparent objects. The WUTScope is distinguished its compact design capability to operate under partially coherent illumination, using polarization diffraction gratings...
<title>Abstract</title> Extracellular vesicles (EVs) are emerging as one of the most promising candidates for novel drug delivery systems (DDS). Unique features, like biocompatibility, encapsulation capability and functionalization precise targeting make them an attractive biomedical applications. However, despite numerous benefits, progress in EVs-based DDS translation from bench to clinics remains highly challenging. One key limitations that needs be addressed low production yield, limited...
The aim of this paper is to present a novel method, called Adaptive Edge Detection (AED), extraction precise pupil edge coordinates from eye image characterized by reflections external illuminators and laser beams. method used for monitoring size position during psychophysical tests two-photon vision performed dedicated optical set-up. Two-photon new phenomenon perception short-pulsed near infrared beams focused on human retina as having color half their wavelength it caused absorption...
In this paper, we present a fibre-optic sensor for simultaneous measurement of refractive index and thickness liquid layers.We designed an experimental low-coherence setup with two broadband light sources extrinsic Fabry-Pérot interferometer acting as the sensing head.We examined how film its affect spectrum at output interferometer.We performed series experiments using only one head.The spectra were collected in ranges 1220÷1340 nm 1500÷1640 nm.The obtained results show that recorded...
In this paper, an investigation into detecting immunosuppressive medicine in aqueous solutions using a spectrometry-based technique is described. Using optical transmissive spectrometry, absorbance measurements the spectra range from 250 nm to 1000 were carried out for different cyclosporine A (CsA) concentrations solutions. The experiment was conducted samples both with and without interferent substances—glucose sodium chloride. dedicated algorithm, measured data analyzed high correlation...
Two-photon vision relies on the perception of pulsed near-infrared laser beams as having colors like their half-wavelength counterparts. The phenomenon is due to two-photon absorption occurring in visual pigments [1]. This study focused methods determine contrast sensitivity function (CSF) for vision, which has not yet been investigated. CSF was measured eight spatial frequencies using tumbling E letter optotype. optotype projected a white background by fast scanning retina with 1040 nm or...
In this paper, a study of low-coherence fiber optic displacement sensor is presented. The consisted broadband source whose central wavelength was either at 1310 nm or 1550 nm, sensing Fabry-Pérot interferometer operating in reflective mode and an optical spectrum analyzer acting as the detection setup. All these components were connected by single-mode coupler. Metrological parameters investigated for different lengths connecting (1 m 10 m). For each length fiber, range 0 μm to 500 μm,...
In this paper, we describe how commonly present in tissue substances - sodium chloride and glucose affect the optical spectrum of an immunosuppressive drug (Cyclaid® by Apotex). Prepared samples were investigated using spectrophotometer range from 250 nm to 1100 nm. The maximum wavelength shift calculated on basis measurement results is not bigger than step. So, it can be concluded, that small amount or does influence possible apply spectrophotometry a point-of-care sensor Cyclaid® Apotex...
The detection of molecules by surface-enhanced Raman spectroscopy (SERS) is dependent on the nanomaterial used to induce enhancement effect. This depends a variety parameters substrate such as metal for their creation, shape, size and distribution, concentration, well solution, packing nanoparticles, complexity sample, solvent, etc. It most crucial, that are kept constant provide uniformity enhancement. this crucial development SERS reliable quantitative technique bioanalysis. Here, we have...
In this paper, we present the preliminary results of scotopic luminosity curve for two-photon vision measurements in spectral range from 872 nm to 1027 nm. The were obtained thanks a newly-developed custom-build tunable femtosecond erbium-doped fiber laser that pulse train parameters and width are close constant while tuning. Such instrumentation enabled us perform reliable across tuning over 150
Get PDF Email Share with Facebook Tweet This Post on reddit LinkedIn Add to CiteULike Mendeley BibSonomy Citation Copy Text J. Bogusławski, G. Palczewska, M. Dąbrowski, S. Tomczewski, Milkiewicz, D. Stachowiak, K. Komar, Marzejon, B. L. Sikorski, A. Hudzikowski, Głuszek, Z. Łaszczych, Soboń, Palczewski, and Wojtkowski, "Two-photon excited fluorescence scanning laser ophthalmoscope for in vivo imaging of the human eye," Biophotonics Congress: Biomedical Optics 2022 (Translational, Microscopy,...
The two- and one-photon visual sensitivities (2phS 1phS) of cataract patients were tested before after IOL surgery. Mean change in 2phS was twice smaller than 1phS indicating that is less affected by cataract.