A5036693743- Single-cell and spatial transcriptomics
- Retinal Development and Disorders
- Neuroinflammation and Neurodegeneration Mechanisms
- Plant tissue culture and regeneration
- Cell Image Analysis Techniques
- Plant Genetic and Mutation Studies
- RNA Interference and Gene Delivery
- Advanced Fluorescence Microscopy Techniques
- Advanced biosensing and bioanalysis techniques
- Quantum Dots Synthesis And Properties
- Photosynthetic Processes and Mechanisms
- Immune Cell Function and Interaction
- Engineering Education and Pedagogy
- HIV Research and Treatment
- Experimental Learning in Engineering
- Zebrafish Biomedical Research Applications
- interferon and immune responses
- Neural dynamics and brain function
- Neurogenesis and neuroplasticity mechanisms
- Neurobiology and Insect Physiology Research
- Transgenic Plants and Applications
- Biomedical and Engineering Education
- T-cell and B-cell Immunology
University of California, Berkeley
2020-2025
QB3
2021-2023
Neuroscience Institute
2021
University of Michigan
2019
Rare CD4 T cells that contain HIV under antiretroviral therapy represent an important barrier to cure
The genesis of broad neuronal classes from multipotential neural progenitor cells has been extensively studied, but less is known about the diversification a single class into multiple types. We used single-cell RNA-seq to study how newly born (postmitotic) mouse retinal ganglion cell (RGC) precursors diversify ~45 discrete Computational analysis provides evidence that RGC transcriptomic type identity not specified at mitotic exit, acquired by gradual, asynchronous restriction postmitotic...
How early sensory experience during “critical periods” of postnatal life affects the organization mammalian neocortex at resolution neuronal cell types is poorly understood. We previously reported that functional and molecular profiles layer 2/3 (L2/3) in primary visual cortex (V1) are vision-dependent [S. Cheng et al. , Cell 185 311–327.e24 (2022)]. Here, we characterize spatial L2/3 with without experience. Spatial transcriptomic profiling based on 500 genes recapitulates zonation along...
The development and connectivity of retinal ganglion cells (RGCs), the retina's sole output neurons, are patterned by activity-independent transcriptional programs activity-dependent remodeling. To inventory molecular correlates these influences, we applied high-throughput single-cell RNA sequencing (scRNA-seq) to mouse RGCs at six embryonic postnatal ages. We identified temporally regulated modules genes that correlate with, likely regulate, multiple phases RGC development, ranging from...
ABSTRACT Mouse whisker somatosensory cortex (wS1) is a major model system to study the experience-dependent plasticity of cortical neuron physiology, morphology, and sensory coding. However, role experience in regulating neuronal cell type development gene expression wS1 remains poorly understood. We assembled annotated transcriptomic atlas during postnatal comprising 45 molecularly distinct types that can be grouped into eight excitatory four inhibitory subclasses. Using this atlas, we...
Abstract How early sensory experience during “critical periods” of postnatal life affects the organization mammalian neocortex at resolution neuronal cell types is poorly understood. We previously reported that functional and molecular profiles layer 2/3 (L2/3) in primary visual cortex (V1) are vision-dependent (Tan et al., Neuron , 108 (4), 2020; Cheng Cell 185 (2), 2022). Here, we characterize spatial L2/3 with without experience. Spatial transcriptomic profiling based on 500 genes...
Abstract Rapidly growing interest in nanoparticle-mediated delivery of DNA and RNA to plants requires a better understanding how nanoparticles their cargoes translocate plant tissues into cells. However, little is known about the size shape influences transport use cargoes, limiting development deployment nanotechnology systems. Here, we employ non-biolistically delivered DNA-modified gold (AuNP) spanning various sizes (5 – 20 nm) shapes (spheres rods) systematically investigate following...
Summary Retinal ganglion cells (RGCs) form an array of feature detectors, which convey visual information to central brain regions. Characterizing RGC diversity is required understand the logic underlying functional segregation. Using single-cell transcriptomics, we systematically classified RGCs in adult and larval zebrafish, thereby identifying marker genes for at least 33 stable transient cell types. We used this dataset engineer transgenic driver lines, enabling experimental access...
ABSTRACT The role of postnatal experience in sculpting cortical circuitry, while long appreciated, is poorly understood at the level cell types. We explore this mouse primary visual cortex (V1) using single-nucleus RNA-sequencing, deprivation, genetics, and functional imaging. find that vision selectively drives specification glutamatergic types upper layers (L) (L2/3/4), deeper-layer glutamatergic, GABAergic, non-neuronal are established prior to eye opening. L2/3 form an...
Abstract The genesis of broad neuronal classes from multipotential neural progenitor cells has been extensively studied, but less is known about the diversification a single class into multiple types. We used single-cell RNA-seq to study how newly-born (postmitotic) mouse retinal ganglion cell (RGC) precursors diversify ~45 discrete Computational analysis provides evidence that RGC type identity not specified at mitotic exit, acquired by gradual, asynchronous fate restriction postmitotic...
ABSTRACT The development and connectivity of retinal ganglion cells (RGCs), the retina’s sole output neurons, are patterned by activity-independent transcriptional programs activity-dependent remodeling. To inventory molecular correlates these influences, we applied high-throughput single-cell RNA sequencing (scRNA-seq) to mouse RGCs at six embryonic postnatal ages. We identified temporally regulated modules genes that correlate with, likely regulate, multiple phases RGC development, ranging...
The interdisciplinary roots of chemical engineering have shaped its history and fostered rapidly evolving nature. Through ongoing research, graduate students in departments remain abreast the field’s evolution. Yet core undergraduate curricula often fall short introducing to breadth current research field, rendering undergraduates underprepared for elements modern discipline that is currently defining. Herein, we discuss a strategy supplementing by topics skills through an undergraduate-led course.