The 16S rRNA gene works as a rapid and effective marker for the identification of microorganisms in complex communities; hence, huge number microbiomes have been surveyed by amplicon-based sequencing. resolution is always considered only at genus level; however, it has not verified on wide range microbes yet. To fully explore ability potential microbial profiling, here, we propose Qscore, comprehensive method to evaluate performance amplicons integrating amplification rate, multitier...

Viruses can inhabit their hosts in the form of an ensemble various mutant strains. Reconstructing a robust consensus representation for these diverse strains is essential recognizing genetic variations among and delving into aspects like virulence, pathogenesis, selecting therapies. Virus genomes are typically small, often composed only few thousand to several hundred nucleotides. While constructing high-quality virus might seem feasible, most current assemblers generated fragmented contigs....

Abstract Motivation The discontinuous transcription mechanism of coronaviruses contributes to their adaptation different host environments and plays a critical role in lifecycle. Accurate assembly coronavirus transcripts is vital for understanding the virus’s biological traits developing precise prevention treatment strategies. However, existing de novo algorithms are primarily designed alternative splicing events eukaryotes not suitable assembling transcriptome, which consists both genomic...

Angiogenesis and lymphangiogenesis are thought to play a role in the pathogenesis of inflammatory bowel diseases (IBD). However, it is not understood if pathological consequence or productive attempt resolve inflammation. This study investigated effect on intestinal inflammation by overexpressing factor, vascular endothelial growth factor-C (VEGF-C), mouse model acute colitis. Forty eight-week-old female C57BL/6 mice were treated with recombinant adenovirus VEGF-C VEGF-C156S protein. Acute...

Dietary nutrients can be converted by the gut microbiota into metabolites such as short-chain fatty acids, which may serve disease-preventing agents in hypertension. Due to limited population mobility and unique high-calorie dietary habits, cohort of this study a representative for elucidating associations between hypertension under diet.

Recent advances in RNA-seq technology have made identification of expressed genes affordable, and thus boosting repaid development transcriptomic studies. Transcriptome assembly, reconstructing all transcripts from reads, is an essential step to understand genes, proteins, cell functions. assembly remains a challenging problem due complications splicing variants, expression levels, uneven coverage sequencing errors. Here, we formulate the transcriptome as path extraction on graphs (or...

Beta-diversity quantitatively measures the difference among microbial communities thus enlightening association between microbiome composition and environment properties or host phenotypes. The beta-diversity analysis mainly relies on distances microbiomes that are calculated by all features. However, in some cases, only a small fraction of members community plays crucial roles. Such tiny proportion is insufficient to alter overall distance, which always missed end-to-end comparison. On...

Colorectal cancer is one of the most common gastrointestinal cancers. 90% patients in early stages colorectal can survive at least 5 years. However, are diagnosed terminal due to insignificant symptoms times and traumatic pathological diagnosis methods. Previous studies have reported that gut microbiome differs between healthy patients, which opens a new door non-invasive diagnostic The key prerequisite for detection using identification effective markers. In this mini-review, we summarized...

To study the correlation between copy number variations of CCND1 gene and chromosome 11 their associations with clinicopathologic features in acral melanoma.

<title>Abstract</title> Viruses can inhabit their hosts in the form of an ensemble various mutant strains. Reconstructing a robust consensus representation for these diverse strains is essential recognizing genetic variations among and delving into aspects like virulence, pathogenesis, selecting therapies. Virus genomes are typically small, often composed only few thousand to several hundred nucleotides. While constructing high-quality virus might seem feasible, most current assemblers...

<title>Abstract</title> <bold>Background:</bold> Discontinuous transcription allows coronaviruses to efficiently replicate and transmit within host cells, enhancing their adaptability survival. Assembling viral transcripts is crucial for virology research the development of antiviral strategies. However, traditional transcript assembly methods primarily designed variable alternative splicing events in eukaryotes are not suitable problem. The current algorithms assembling often struggle with...

Discontinuous transcription allows coronaviruses to efficiently replicate and transmit within host cells, enhancing their adaptability survival. Assembling viral transcripts is crucial for virology research the development of antiviral strategies. However, traditional transcript assembly methods primarily designed variable alternative splicing events in eukaryotes are not suitable problem. The current algorithms assembling often struggle with low accuracy determining boundaries. There an...

Objective: To investigate the clinical manifestations, pathological features, diagnosis and treatment of myeloid sarcoma, to improve understanding sarcoma. Methods: The data, 7 patients with sarcoma were retrospectively analyzed. Results: Of 1 was male 6 female. In most patients, local compression symptoms caused by painless masses or first manifestations. One patient had lesions involving cervix vaginal bleeding symptom. extensive 19 sites involved. positive proportion immunohistochemical...

The methylation of cytosine is one the most fundamental epigenetic modifications in mammalian genomes, and involved multiple crucial processes including gene expression, cell differentiation, embryo development oncogenesis. In past, DNA was thought to be an irreversible process, which could only diluted passively through replication. It now becoming increa-singly obvious that demethylation can active process plays a role biological processes. Ten eleven translocation (TET) proteins are key...

黑色素瘤恶性程度高，发病机制复杂。细胞周期蛋白D1（cyclin D1）和细胞周期蛋白依赖性激酶4（cyclin-dependent kinases 4，CDK4）是细胞周期进程中的重要分子，可形成复合物发挥功能促进细胞从G1期进入S期。cyclin D1和CDK4的异常在黑色素瘤，尤其是肢端黑色素瘤中常见，与黑色素瘤的发生关系密切。cyclin D1在黑色素瘤中的异常主要表现为其编码基因CCND1扩增和蛋白表达升高。CDK4在黑色素瘤中的异常可表现为基因扩增或点突变。了解cyclin D1和CDK4在黑色素瘤中的异常，有助于良恶性黑色素细胞肿瘤的鉴别诊断，也可为CDK4抑制剂在黑色素瘤治疗中的作用提供参考，并可能有助于靶向治疗患者的筛选。.

Objective: Recently, increasing number of lung cancer patients benefit from immune-checkpoint inhibitors (ICIs). However, the data Chinese small cell (SCLC) is limited. This study aims to analyze response and survival ICIs treatment in SCLC explore predictive biomarkers. Methods: Forty-seven who received Peking University Cancer Hospital May 2017 September 2019 was recruited. Clinical characteristics including sex, age, smoking status, strategy, PD-L1 expression therapeutic efficacy were...