This study was conducted to explore the mechanism by which caffeine increases GLUT4 expression in C(2)C(12) myotubes. Myoblasts were differentiated DMEM containing 2% horse serum for 13 days and resultant myotubes exposed 10 mM presence or absence of 25 microM KN93 dantrolene 2 h. After treatment, cells kept serum-free medium harvested between 0 6 h later, depending on assay. Chromatin immunoprecipitation (ChIP) assays revealed that treatment caused hyperacetylation histone H3 at myocyte...

In this research, hydrogel biocomposites were prepared from whey protein isolate (WPI), reduced graphene oxide (rGO), and synthetic polymers in varied ratios. Their physicochemical properties evaluated by FTIR, SEM, TGA, AFM, TEM. FTIR spectra revealed significant peaks at 1167 cm−1 for C–O–C peak 1449 O–H bending WPI rGO, respectively. The hydrogels loaded with proguanil hydrochloride chloroquine diphosphate vitro release kinetics of individual drugs the studied. SEM images after drug...

Edible plants such as sweet potato are sources of natural antioxidants that can be exploited in the management and treatment insulin resistance. This present study investigated effects extracts an orange-fleshed on oxidative stress biomarkers (glutathione status lipid peroxidation) activities antioxidant enzymes (catalase, CAT glutathione peroxidase, GPx) palmitate-induced resistant C2C12 cells. The intracellular cells was also measured using Ferric reducing power (FRAP) Trolox equivalent...

Metabolite extraction methods have been shown to be a critical consideration for pharmacometabolomics studies and, as such, optimization and development of new are crucial. In the current study, an organic solvent-free method, namely, pressurised hot water (PHWE), was used extract pharmacologically important metabolites from dried Moringa oleifera leaves. Here, temperature solvent (pure water) altered while keeping other factors constant using homemade PHWE system. Samples extracted at...

AMP-activated protein kinase (AMPK) is known to regulate both glucose and lipid metabolism, which play vital roles in the development of metabolic syndrome. One way regulating AMPK through hormonal activation using adiponectin. Patients diagnosed with type-2 diabetes (T2D) obesity exhibit low adiponectin concentration levels their blood. Moreover, studies have also shown that inflammatory processes a significant role etiology these diseases. In this study, long-term effects neonatal intake...

Abstract Dietary manipulations during the early postnatal period are associated with development of metabolic disorders including non-alcoholic fatty liver disease (NAFLD) or long-term protection against dysfunction. We investigated potential hepatoprotective effects neonatal administration oleanolic acid (OA), a phytochemical, on subsequent in adulthood, dietary fructose-induced NAFLD. Male and female suckling rats ( n =112) were gavaged with; distilled water (DW), OA (60 mg/kg), high...

Diabetes mellitus is a metabolic disease that poses serious global health challenge. The provision of alternative and complementary medicine to combat the has attracted attention numerous scientists. In this study, anti- α-glucosidase activities selected seven compounds obtained from Phyllanthus amarus leaves were explored using computational chemistry approach. absorption, distribution, metabolism excretion (ADME) properties compound with best binding affinity metformin examined....

Metabolic syndrome, a cluster of different disorders which include diabetes, obesity and cardiovascular diseases, is global epidemic that growing at an alarming rate. The origins disease can be traced back to early developmental stages life. This has increased mortalities continues reduce life expectancies individuals across the globe. aim this study was investigate sub-acute long term effects neonatal oral administration oleanolic acid metformin on lipids (free fatty acids, FFAs) genes...

Consumption of fructose-rich diets has been implicated in the increasing global prevalence metabolic syndrome (MetS). Interventions during periods early ontogenic developmental plasticity can cause epigenetic changes which program metabolism for positive or negative health benefits later life. The phytochemical oleanolic acid (OA) possesses anti-diabetic and anti-obesity effects. We investigated potential protective effects neonatal administration OA on subsequent development high fructose...

South Africa. This study investigated the nutritional and chemical compositions of four whitefleshed (Blesbok, Ndou, Monate, Mvuvhelo) three orange-fleshed (Bophelo, Impilo, 199062.1) cultivars. The protein, ash, crude fibre contents were analysed using standard AOAC procedures. detection fatty acids, sugar, metabolites was carried out Gas Chromatography–Mass Spectrometry (GC-MS). Carbohydrate energy values determined methods. results showed that Bophelo had highest protein content...

Nutritional manipulations in the neonatal period are associated with development of negative or positive health outcomes later life. Excessive fructose consumption has been attributed to increase global prevalence metabolic syndrome (MetS) and oxidative stress. Oleanolic acid (OA) anti-diabetic anti-obesity effects. We investigated protective potential orally administering OA period, prevent fructose-induced stress, adverse maturation gastrointestinal tract (GIT) suckling rats. Seven-day old...

Activation of calmodulin dependent protein kinase (CaMK)II by exercise is beneficial in controlling membrane lipids associated with type 2 diabetes and obesity. Regulation lipid metabolism crucial the improvement obesity symptoms. The role CaMKII was focus this study. Five to six weeks old male Wistar rats were used GC×GC-TOFMS technique determine levels polyunsaturated fatty acids (linoleic acid, arachidonic acid 11,14-eicosadienoic acid). Carnitine palmitoyltransferase (Cpt-1) acetyl-CoA...