A5081035670- Reproductive tract infections research
- Probiotics and Fermented Foods
- Gut microbiota and health
- Urinary Tract Infections Management
- Advanced Drug Delivery Systems
- Antimicrobial Peptides and Activities
- Wound Healing and Treatments
- Bacterial biofilms and quorum sensing
- Bee Products Chemical Analysis
- Oral microbiology and periodontitis research
- Antifungal resistance and susceptibility
- Dermatology and Skin Diseases
- Antimicrobial agents and applications
- Pelvic floor disorders treatments
- Cervical Cancer and HPV Research
- Pediatric health and respiratory diseases
- Advanced Chemical Sensor Technologies
- Protein Hydrolysis and Bioactive Peptides
- Laser Applications in Dentistry and Medicine
- Oral Health Pathology and Treatment
- Microencapsulation and Drying Processes
- Advancements in Transdermal Drug Delivery
- Food composition and properties
- Pharmaceutical studies and practices
- Inhalation and Respiratory Drug Delivery
University of Bologna
2017-2024
UiT The Arctic University of Norway
2019
Successful treatment of skin infections requires eradication biofilms found in up to 90 % all chronic wounds, causing delayed healing and increased morbidity. We hypothesized that chitosan hydrogel boosts the activity liposomally-associated membrane active antimicrobials (MAA) could potentially improve bacterial biofilm eradication. Therefore, liposomes (∼300 nm) bearing chlorhexidine (CHX; ∼50 μg/mg lipid) as a model MAA were incorporated into hydrogel. The novel CHX-liposomes-in-hydrogel...
Abstract Background Lactobacillus spp. dominating the vaginal microbiota of healthy women contribute to prevention urogenital and sexually transmitted infections. Their protective role in vagina can be mediated by cells themselves, metabolites or bacterial components, able interfere with pathogen adhesion infectivity. Vulvovaginal candidiasis (VVC) is a common genital infection, caused overgrowth opportunistic Candida including C. albicans, glabrata, krusei tropicalis . Azole antifungal...
Vulvovaginal candidiasis (VVC) is a widely spread fungal infection that causes itching, pain and inflammation at the vaginal site. Although common, currently available treatment suffers from limited efficacy high recurrence. In addition, growing problem of resistance to azole drugs used in current treatments emphasizes need for superior options. Antimicrobial polyphenols are an attractive approach offering multitargeting therapy. We aimed develop novel liposomes simultaneous delivery two...
Lactobacilli are the dominant members of healthy human vaginal microbiota and represent first defense line from pathogen infection, including vulvovaginal candidiasis. Biofilm is predominant microbial growth form in nature, formation biofilms inside body has important implications health disease. In particular, biofilm by resident desirable, as it can improve persistence influence functionality. present study, we investigated capability 16 Lactobacillus strains (belonging to crispatus,...
Lactobacillus species dominate the vaginal microflora performing a first-line defense against infections. Extracellular vesicles (EVs) released by lactobacilli are considered mediators of their beneficial effects affecting cellular communication, homeostasis, microbial balance, and host immune system pathways. Up to now, very little is known about role played EVs in microenvironment, mechanisms action remain poorly understood.Here, we hypothesized that can mediate modulating microbiota...
The vaginal microbiota plays a crucial role in maintaining the health and functioning of female genital tract, preventing colonization urogenital pathogens sexually transmitted infections. In this study, we characterized bacterial communities metabolome associated to Chlamydia trachomatis infection (CT: 20 women), compared healthy condition (H: 22 women) vaginosis (BV: 19 women). A microarray-based tool (VaginArray), implemented with real-time PCR for Gardnerella vaginalis, was used...
Lactobacilli represent a wide range of bacterial species with several implications for the human host. They play crucial role in maintaining ecological equilibrium different biological niches and are essential fermented food production probiotic formulation. Despite consensus about 'health-promoting' significance Lactobacillus genus, its genotypic phenotypic characterization still poses difficulties. The aim this study was to assess integration approaches, (16S rRNA gene sequencing),...
Lactobacilli play a crucial role in maintaining the ecological equilibrium of vaginal niche, preventing colonization exogenous microorganisms. Although many studies have discussed mechanisms displayed by lactobacilli counteracting several urogenital pathogens, few data are available on interaction between and Chlamydia trachomatis. This study aimed to elucidate molecular bases among lactobacilli, sexually transmitted pathogen C. trachomatis epithelial cervical cells. We evaluated vitro...
Exopolysaccharides (EPS) secreted by beneficial lactobacilli exert a plethora of positive activities, but little is known about their effects on biofilms opportunistic vaginal pathogens and especially themselves. Here, the EPS produced six lactobacilli, belonging to Lactobacillus crispatus (BC1, BC4, BC5) gasseri (BC9, BC12, BC14) species were isolated from cultural supernatants lyophilized.Lactobacillus chemically characterized in terms monosaccharide composition liquid chromatography (LC)...
The emergence of resistance to antifungal drugs has made the treatment vulvovaginal candidiasis (VVC) very challenging. Among natural substances, biosurfactants (BS) produced by Lactobacillus have gained increasing interest in counteracting Candida infections for their proven anti-adhesive properties and safety profile. In present study, liposomes (LP-BS) or coated with hyaluronic acid (HY-LP-BS) were prepared presence BS isolated from vaginal strain crispatus BC1 characterized terms size, ζ...
The eradication of bacteria embedded in biofilms is among the most challenging obstacles management chronic wounds. These are found wounds; moreover, biofilm-embedded considerably less susceptible to conventional antimicrobial treatment than planktonic bacteria. Antimicrobial peptides and their mimics considered attractive candidates pursuit novel therapeutic options for wounds general bacterial eradication. However, some limitations linked these membrane-active antimicrobials making...
The potential of whey from Italian cheese productions to support skin health and anti-ageing mechanism was studied. effect on dermal epidermal cells evaluated. Whey inhibited the activity elastase tyrosinase enzymes by 60% 32%, respectively. cytotoxicity against tested cell lines, human fibroblasts (HDFa) keratinocytes (HaCaT) not observed. Moreover, antioxidant samples noted, after treatment with intracellular ROS level decreased 87% in comparison hydrogen peroxide-treated cells....
A healthy vaginal microbiota hosts Lactobacillus as the most predominant genus. Lactobacilli play a role in human health through production of diverse antimicrobial substances that can act against pathogens or modulate immune system. Previous reports highlighted ability lactobacilli to counteract viruses causing STIs, e.g., HIV-1 and HSV-2. In this report, we analyze activity supernatants HSV-1 infection, which is becoming increasingly relevant STI. We show two species (i.e., L. crispatus...
The inclusion of a chemical permeation enhancer in dosage form is considered an effective approach to improve absorption across the nasal mucosa. Herein we evaluated possibility exploiting biosurfactants (BS) produced by Lactobacillus gasseri BC9 as innovative natural excipients delivery hydrocortisone (HC). BC9-BS ability HC solubility and BS mucoadhesive potential were investigated using surfactant at concentration below above critical micelle (CMC). In vitro diffusion studies through...
Vaginal lactobacilli offer protection against recurrent urinary and vaginal infections. The precise mechanisms underlying the interaction between host epithelium remain poorly understood at molecular level. Deciphering such events can provide valuable information on mode of action commensal probiotic bacteria in environment. We investigated effects exerted by five Lactobacillus strains origin ( crispatus BC1 BC2, gasseri BC9 BC11 vaginalis BC15) physical properties plasma membrane a cervical...
The gut of babies born vaginally is rapidly colonized by Bifidobacterium spp. after birth, while in infants cesarean section (C-section), the presence bifidobacteria drops dramatically, increasing risk developing gastrointestinal disorders. Considering that newborns naturally come into contact with maternal lactobacilli as they pass through birth canal, aim this work to exploit for first time bifidogenic activity exerted cell-free supernatants (CFSs) from vaginal origin, belonging species...