A5000698962- Leptospirosis research and findings
- Cellular transport and secretion
- Advanced Electron Microscopy Techniques and Applications
- Nuclear Structure and Function
- Endoplasmic Reticulum Stress and Disease
- Toxin Mechanisms and Immunotoxins
- RNA Research and Splicing
- Lipid Membrane Structure and Behavior
- Fungal and yeast genetics research
- Biochemical and Molecular Research
- Plant and Biological Electrophysiology Studies
- Brucella: diagnosis, epidemiology, treatment
- Advanced Fluorescence Microscopy Techniques
- Infections and bacterial resistance
- Electrospun Nanofibers in Biomedical Applications
- Angiogenesis and VEGF in Cancer
- Amoebic Infections and Treatments
- Microtubule and mitosis dynamics
- Plant Parasitism and Resistance
- Prion Diseases and Protein Misfolding
- Tissue Engineering and Regenerative Medicine
- Genetic Neurodegenerative Diseases
- Neuroscience and Neuropharmacology Research
- Biofuel production and bioconversion
- Parkinson's Disease Mechanisms and Treatments
Yale University
2017-2022
Max Planck Institute of Molecular Cell Biology and Genetics
2011-2015
Max Planck Society
2011
One of the key questions in biology is how metabolism a cell responds to changes environment. In budding yeast, starvation causes drop intracellular pH, but functional role this pH change not well understood. Here, we show that enzyme glutamine synthetase (Gln1) forms filaments at low and filament formation leads enzymatic inactivation. Filament by Gln1 highly cooperative process, strongly dependent on macromolecular crowding, involves back-to-back stacking cylindrical homo-decamers into...
Membrane curvature sensors have diverse structures and chemistries, suggesting that they might the intrinsic capacity to discriminate between different types of vesicles in cells. In this paper, we compare vitro vivo membrane-binding properties two form very amphipathic helices: lipid-packing sensor (ALPS) motif a Golgi vesicle tether synaptic protein α-synuclein, causative agent Parkinson's disease. We demonstrate mechanism by which α-synuclein senses membrane curvature. Unlike ALPS motifs,...
Significance Proteins carrying aggregation-prone prion-like domains cause many neurodegenerative diseases. The presence of prions in yeast has provided important insights into these disease processes and mechanisms cellular proteostasis. However, it is not known whether findings extend to other organisms. In this paper, we show that Dictyostelium discoideum the highest content proteins all organisms investigated date. Remarkably, overexpressed remain soluble are innocuous D. , contrast...
Spirochete bacteria, including important pathogens, exhibit a distinctive means of swimming via undulations the entire cell. Motility is powered by rotation supercoiled 'endoflagella' that wrap around cell body, confined within periplasmic space. To investigate structural basis flagellar supercoiling, which critical for motility, we determined structure native filaments from spirochete Leptospira integrating high-resolution cryo-electron tomography and X-ray crystallography. We show these...
The nuclear envelope (NE) assembles and grows from bilayer lipids produced at the endoplasmic reticulum (ER). How ER membrane incorporation coordinates with assembly of pore complexes (NPCs) to generate a functional NE is not well understood. Here, we use stereotypical first division early C. elegans embryo test role membrane-associated nucleoporin Ndc1 in coupling NPC formation growth. 3D-EM tomography reforming expanded NEs establishes that determines density. Loss ndc1 results faster...
The spirochete endoflagellum is a unique motility apparatus among bacteria. Despite its critical importance for pathogenesis, the full composition of flagellum remains to be determined. We have recently reported that FcpA novel flagellar protein and major component sheath filament Leptospira. By screening library random transposon mutants in Leptospira biflexa, we found motility-deficient mutant harboring disruption hypothetical gene unknown function. Here, show this encodes surface...
SUMMARY One of the key questions in biology is how metabolism a cell responds to changes environment. In budding yeast, starvation causes drop intracellular pH, but functional role this pH change not well understood. Here, we show that enzyme glutamine synthetase (Gln1) forms filaments at low and filament formation leads inactivation. Filament by Gln1 highly cooperative process, strongly dependent on macromolecular crowding, involves back-to-back stacking cylindrical homo-decamers into...
The Rab GTPase‐activating proteins (GAP) Gyp5p and Gyl1p are involved in the control of polarized exocytosis at small‐bud stage Saccharomyces cerevisiae . Both interact with N‐Bin1/Amphiphysin/Rvs167 (BAR) domain protein Rvs167p, but biological function this interaction is unclear. We show here that recruit Rvs167p to tip, where it plays a role exocytosis. In gyp5 Δ gyl1 cells, not correctly localized tip. P473L mutation SH3 deletion proline‐rich regions disrupt impair localization tips...
Abstract Spirochete bacteria, including important pathogens, exhibit a distinctive means of swimming via undulations the entire cell. Motility is powered by rotation supercoiled ‘endoflagella’ that wrap around cell body, confined within periplasmic space. To investigate structural basis flagellar supercoiling, which critical for motility, we determined structure native filaments from spirochete Leptospira integrating high-resolution cryo-electron tomography and X-ray crystallography. We show...
Abstract Several Leptospira species are bacterial agents of leptospirosis, a neglected tropical disease responsible for ~ 1 million cases and 50,000 deaths each year worldwide. , like other members the Spirochaeta phylum, possess specially adapted flagella that remain confined within periplasm. These appendages drive unique, corkscrew-like swimming style enables efficient motility pathogenesis. However, composition, function, molecular architecture spirochetal flagellar filaments poorly...
Summary Nuclear pore complexes (NPCs) are large protein assemblies that facilitate transport of macromolecules across the nuclear envelope (NE) [1, 2]. How thousands NPCs rapidly assemble after open mitosis to form a functional NE is not known. Recruitment Nup107-160 outer ring scaffold chromatin initiates NPC assembly. The Nup53/93 complex bridges central channel [3–6]. Nup53 interacts with conserved transmembrane nucleoporin Ndc1; however, how Ndc1 contributes post-mitotic assembly unclear...