A5072227102- Cellular Mechanics and Interactions
- Bone Metabolism and Diseases
- Bone health and osteoporosis research
- Nanofabrication and Lithography Techniques
- 3D Printing in Biomedical Research
- Nanotechnology research and applications
- Bone health and treatments
- Microfluidic and Bio-sensing Technologies
- Force Microscopy Techniques and Applications
- Erythrocyte Function and Pathophysiology
- Mechanical and Optical Resonators
- Analytical Chemistry and Sensors
- Advanced MEMS and NEMS Technologies
- Nanowire Synthesis and Applications
- Nanopore and Nanochannel Transport Studies
- Cancer Cells and Metastasis
- Laser-Ablation Synthesis of Nanoparticles
- Gas Sensing Nanomaterials and Sensors
- Graphene and Nanomaterials Applications
- Advanced biosensing and bioanalysis techniques
- Micro and Nano Robotics
- Electrospun Nanofibers in Biomedical Applications
- Integrated Circuits and Semiconductor Failure Analysis
- Molecular Junctions and Nanostructures
- Quantum Dots Synthesis And Properties
University of Toronto
2016-2025
Queen's University
2024-2025
University of New Brunswick
2008-2011
Pennsylvania State University
2010
Villanova University
2010
Stanford University
2003-2008
Rehabilitation Research and Development Service
2006
City College of New York
2001-2004
Veterans Health Administration
2004
Palo Alto Veterans Institute for Research
2004
Abstract Osteocytes are believed to be the mechanical sensor cells in bone. One potential physical mechanism for mechanosensing process is that osteocytes directly sense deformation of substrate which they attached. However, there a fundamental paradox this theory: tissue‐level strains whole bone typically <0.2%, yet an extensive range vitro experiments show dynamic must at least order magnitude larger intracellular biochemical responses occur. Recently, theoretical model was developed...
Mechanical loading is an important regulator of bone formation and loss. Decreased osteoblast number function are cellular mechanisms by which mechanical disuse leads to decreased formation. may be a result osteoprogenitor proliferation, differentiation, or both. However, the effects level physical signals on osteoprogenitors not well understood. In this study, we examined induced oscillatory fluid flow (OFF), potent osteoblastic cell function, marrow stromal cells (MSCs). MSCs subjected OFF...
This paper presents a microfluidic system for cell type classification using mechanical and electrical measurements on single cells. Cells are aspirated continuously through constriction channel with elongations impedance profiles measured simultaneously. The transit time the amplitude ratio quantified as cell's property indicators. device measurement were used to characterize osteoblasts (n = 206) osteocytes 217), revealing that osteoblasts, compared osteocytes, have larger elongation...
Semiconductive nanowire-based biosensors are capable of label-free detection biological molecules. Nano-FET (field-effect transistor) exhibiting high sensitivities toward proteins, nucleic acids, and viruses have been demonstrated. Rational device design methodologies, particularly those based on theoretical predictions, were reported. However, few experimental studies investigated the effect nanowire diameter, doping density, number nano-FET sensitivity. In this study, we devised a...
Fatigue loading causes a spatial distribution of osteocyte apoptosis co-localized with bone resorption spaces peaking around microdamage sites. Since osteocytes have been shown to regulate osteoclast formation and activity, we hypothesize that regulates osteoclastogenesis. In this study, used serum-starvation mimic reduced nutrient transport in microdamaged induce MLO-Y4 osteocyte-like cells; conditioned medium was apply soluble factors released by apoptotic (aOCY) healthy non-apoptotic...
Manipulation of micrometer to millimeter-scale objects is central biotechnological and medical applications involving small-scale robotic devices. Mobile untethered microgrippers have been developed, which use magnetic fields for motion activation grasping. This letter extends the capabilities such by presenting first example reliable autonomous three-dimensional (3-D) micrograsping cargo delivery a microgripper using simple control strategies. endeavor will allow reliably grasp transfer...
Abstract Bone metastasis is a common, yet serious, complication of breast cancer. Breast cancer cells that extravasate from blood vessels to the bone devastate quality by interacting with and disrupting remodeling balance. Although exercise often suggested as intervention strategy mechanical loading during known regulate remodeling, its role in preventing remains unknown. We developed novel vitro microfluidic tissue model investigate osteocytes regulation metastasis. Metastatic MDA-MB-231...
Fluid flow due to loading in bone is a potent mechanical signal that may play an important role adaptation its environment. Previous vitro studies of osteoblastic cells revealed the upregulation cyclooxygenase-2 (COX-2) and c-fos induced by steady fluid depends on change actin polymerization dynamics formation stress fibers. Exposing dynamic oscillatory flow, temporal pattern results from normal physical activity, also known result increased COX-2 expression PGE(2) release. The purpose this...
Abstract Osteocyte apoptosis, associated with reduced interstitial fluid flow, precedes osteoclast precursor recruitment and may aid in the delivery of precursors to remodeling site by promoting angiogenesis. To test association between flow osteocyte osteocyte‐like MLO‐Y4 cells were subjected either oscillatory (10 dynes/cm 2 , 1 Hz) or no conditions without TNF‐α treatment induce apoptosis chemically. Flow protected osteocytes from regardless whether they treated ( p < 0.001) not 0.05)....
Bone metastases, the migration of cancers to bone, occur in 65-80% patients with advanced breast cancer. Metastasized cancer cells interact such as bone-resorbing osteoclasts alter bone remodeling. Exercise, often suggested an intervention for patients, regulates remodeling via osteocytes. Osteocytes also signal endothelial cells, which may affect cell extravasation. Therefore, we hypothesize that mechanically stimulated osteocytes can regulate processes metastasis. To test this, exposed...
Low-magnitude (≤1 g) high-frequency (≥30 Hz) (LMHF) vibration has been shown to enhance bone mineral density. However, its regulation in breast cancer metastasis remains controversial for patients and elder populations. Yoda1, an activator of the mechanosensitive Piezo1 channel, could potentially intensify effect LMHF by enhancing mechanoresponse osteocytes, major mechanosensory cells with high expression Piezo1. In this study, we treated osteocytes mono- (Yoda1 only or only) combined...