A5049195055- Analytical Chemistry and Chromatography
- Migraine and Headache Studies
- Neuroscience and Neuropharmacology Research
- Analytical Methods in Pharmaceuticals
- Advanced Drug Delivery Systems
- Forensic Toxicology and Drug Analysis
- Fatty Acid Research and Health
- Epilepsy research and treatment
- Trigeminal Neuralgia and Treatments
- Drug Solubulity and Delivery Systems
- Inhalation and Respiratory Drug Delivery
- Cannabis and Cannabinoid Research
- Hormonal and reproductive studies
- Pharmacological Effects and Toxicity Studies
- Advancements in Transdermal Drug Delivery
- Mass Spectrometry Techniques and Applications
- Synthesis and biological activity
- Phytoestrogen effects and research
- Nicotinic Acetylcholine Receptors Study
- Crystallization and Solubility Studies
- Mitochondrial Function and Pathology
- Microencapsulation and Drying Processes
- Neuroinflammation and Neurodegeneration Mechanisms
- Computational Drug Discovery Methods
- Morinda citrifolia extract uses
Ospedale San Luigi Gonzaga
2025
University of Turin
2025
University of Modena and Reggio Emilia
2015-2024
Ibero American University
2021
Ospedali Riuniti di Ancona
1978
Patients affected by protocadherin 19 (PCDH19)-female limited epilepsy (PCDH19-FE) present a remarkable reduction in allopregnanolone blood levels. However, no information is available on other neuroactive steroids and the steroidogenic response to hormonal stimulation. For this reason, we evaluated allopregnanolone, pregnanolone, pregnenolone sulfate liquid chromatographic procedures coupled with electrospray tandem mass spectrometry 12 unrelated patients 15 age-matched controls. We also...
Background: Chronic rhinosinusitis with nasal polyps (CRSwNP) and asthma are mutually correlated Type-2 inflammation. Dupilumab is effective in uncontrolled relapsing CRSwNP. However, the precise characterization of inflammation impact previous surgery on clinical outcomes need clarification. Methods: We present a prospective observational study 38 CRSwNP-patient cohort, whose endotype was confirmed after multidisciplinary approach shared among ENTs, pneumologists allergologists. Patients...
Summary Neuroactive steroids are increasingly considered as relevant modulators of neuronal activity. Especially allopregnanolone ( AP ) and pregnenolone sulfate PS have been shown to possess, respectively, anticonvulsant or proconvulsant properties. In view the potential role these steroids, we aimed at evaluating levels in cerebrospinal fluid CSF blood samples obtained from patients with status epilepticus SE ). To this purpose, enrolled 41 affected by subjects investigated for...
Neuroinflammation, whose distinctive sign is the activation of microglia, supposed to play a key role in development and progression neurodegenerative diseases. The aim this investigation was determine levels neurosteroids produced by resting injured BV-2 microglial cells. cells were exposed increasing concentrations rotenone progressively reduce their viability reactive oxygen species (ROS) production. cell significantly reduced 24, 48 72 h after (50–1000 nM) exposure. Concomitantly,...
Abrupt withdrawal from antiepileptic drugs is followed by increased occurrence of epileptic seizures, a phenomenon known as the “rebound effect”. By stopping treatment with levetiracetam (LEV 300 mg/kg/day, n = 15; vs. saline, 15), we investigated rebound effect in adult male Sprague-Dawley rats. LEV was continuously administered using osmotic minipumps, 7 weeks after intraperitoneal administration kainic acid (15 mg/kg). The effects were determined comparing time intervals, treatments, and...
We obtained evidence that mouse BV2 microglia synthesize neurosteroids dynamically to modify neurosteroid levels in response oxidative damage caused by rotenone. Here, we evaluated whether could be produced and altered rotenone the human microglial clone 3 (HMC3) cell line. To this aim, HMC3 cultures were exposed (100 nM) measured culture medium liquid chromatography with tandem mass spectrometry. Microglia reactivity was measuring interleukin 6 (IL-6) levels, whereas viability monitored...
Produced by the mitochondria and endoplasmic reticulum, neurosteroids such as allopregnanolone are neuroprotective molecules that influence various neuronal functions regulate neuroinflammation. They reduced in neurodegenerative diseases, while Wobbler mouse model, its precursor progesterone showed protective effects on motor neuron degeneration. This single-center case-control study included 37 patients with amyotrophic lateral sclerosis (ALS) 28 healthy controls. Cerebrospinal fluid (CSF)...
Abstract Antifouling biocide zinc pyrithione (ZnPT) and its biological fate have received little attention because this compound was assumed not to be persistent in marine ecosystems. An analytical procedure developed that has proved efficient very sensitive extracting ZnPT main secondary products, Zn ionized (PT−), from both seawater samples, namely the gills digestive gland of bioindicator species Mytilus galloprovincialis. Short-term experiments were carried out investigate toxicity...
The aim of this study was to evaluate the potential hair analysis monitor medication adherence in headache patients undergoing chronic therapy. For purpose, following parameters were analyzed: detection rate 23 therapeutic drugs patients' hair, degree agreement between self-reported drug and found whether levels reflected intake reported by patients.The included 93 suffering from primary headaches declaring their daily at least one during 3 months before sampling: alprazolam, amitriptyline,...
Reduced blood or cerebrospinal fluid levels of allopregnanolone are involved in menstrual cycle-linked CNS disorders, such as catamenial epilepsy. This condition, like menstrually-related migraine, is characterized by severe, treatment-resistant attacks. We explored whether there were differences allopregnanolone, progesterone and testosterone serum between women with migraine (MM, n = 30) postmenopausal without aura who had suffered from during their fertile age (PM, non-headache control...
Polypharmacy can appropriately treat multiple chronic conditions, but it also increase potential harm. information for primary headaches is minimal, despite drugs being the main tools to manage headaches. The aim was evaluate prevalence, characteristics and risk factors of polypharmacy in patients with examine whether these variables differ between episodic headache patients. We analysed (simultaneous use five or more medications), medication type, comorbidity, 300 (mean age 42.81 ± 13.21...
Neurosteroids such as allopregnanolone may play a role in epilepsy positive modulators of inhibitory currents mediated by γ-aminobutyric acid type A (GABAA ) receptor. Indeed, these molecules have been consistently shown to be anticonvulsants animal models, but their is still unclear patients. For this reason, we investigated neurosteroids the cerebrospinal fluid (CSF) patients with status epilepticus (SE) liquid chromatography tandem-mass spectrometry. Patients were retrospectively...