A5083379665- Gut microbiota and health
- Clostridium difficile and Clostridium perfringens research
- Probiotics and Fermented Foods
- RNA Interference and Gene Delivery
- Fermentation and Sensory Analysis
- Virus-based gene therapy research
- Intensive Care Unit Cognitive Disorders
- Immune cells in cancer
- Respiratory viral infections research
- Bacterial biofilms and quorum sensing
- Amoebic Infections and Treatments
- Pharmacogenetics and Drug Metabolism
- Salivary Gland Disorders and Functions
- Animal Genetics and Reproduction
- Antifungal resistance and susceptibility
- Clinical Nutrition and Gastroenterology
- Defense, Military, and Policy Studies
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Nail Diseases and Treatments
- Advanced Aircraft Design and Technologies
- Immune Response and Inflammation
- Influenza Virus Research Studies
- Antibiotic Resistance in Bacteria
- Ginseng Biological Effects and Applications
- 3D Printing in Biomedical Research
Merck & Co., Inc., Rahway, NJ, USA (United States)
2021-2024
Brown University
2016-2020
John Brown University
2019
Rhode Island Hospital
2018
University of Massachusetts Chan Medical School
2015
Despite recent interest in microbial communities of fermented foods, there has been little inquiry into the bacterial community dynamics sauerkraut, one world’s oldest and most prevalent foods. In this study, we utilize 16S rRNA amplicon sequencing to profile naturally sauerkraut throughout fermentation process while also analyzing starting ingredients production environment. Our results indicate that microbiome is rapidly established after begins stable through packaging for commercial...
Effective gene delivery to the central nervous system (CNS) is vital for development of novel therapies neurological diseases. Adeno-associated virus (AAV) vectors have emerged as an effective platform in vivo transfer, but overall neuronal transduction efficiency derived from naturally occurring AAV capsids after systemic administration relatively low. Here, we investigated possibility improving CNS existing by genetically fusing peptides N-terminus VP2 capsid protein. A vector AAV-AS,...
Microbial competition between Escherichia coli and Candida albicans reveals a soluble fungicidal factor – Localized systemic fungal infections caused by can lead to significant mortality morbidity. However, severe C. are relatively rare, occurring mostly in the very young, old, immunocompromised individuals. The fact that these rare is interesting because as much 80 percent of population asymptomatically colonized with albicans. It thought members human microbiota immune system work concert...
Abstract Urogenital schistosomiasis is a neglected tropical disease caused by the parasite Schistosoma haematobium , which resides in vasculature surrounding urogenital system. Previous work has suggested that helminthic infections can affect intestinal microbiome, and we hypothesized S . infection could result an alteration of immune system-microbiota homeostasis impact composition gut microbiota. To address this question, compared fecal microbiomes infected uninfected schoolchildren from...
In recent years, a growing amount of research has begun to focus on the oral microbiome due its links with health and systemic disease. The numerous advantages that make it particularly useful for clinical studies, including non-invasive collection, temporal stability, lower complexity relative other niches, such as gut. Despite discoveries made in this area, is unknown how responds short-term hospitalization. Previous studies have demonstrated gut extremely sensitive hospitalization these...
Dietary composition and antibiotic use have major impacts on the structure function of gut microbiome, often resulting in dysbiosis. Despite this, little research has been done to explore role host diet as a determinant antibiotic-induced microbiome disruption. Here, we utilize multi-omic approach characterize impact Western-style consumption ciprofloxacin-induced changes transcriptional activity. We found that Western dramatically increased Bacteroides abundances shifted community toward...
Antibiotic resistance is a current and expanding threat to the practice of modern medicine. therapy has been shown perturb composition host microbiome with significant health consequences. In addition, gut known be reservoir antibiotic genes. Work demonstrated that antibiotics can alter collection genes within through selection horizontal gene transfer. While also have potential impact expression genes, metagenomic-based pipelines currently lack ability detect these shifts. Here, we utilized...
The human microbiota impacts a variety of diseases and responses to therapeutics. Due lack robust in vitro models, detailed mechanistic explanations host-microbiota interactions cannot often be recapitulated. We describe the design development novel, versatile modular system that enables indirect coculture epithelial cells with anaerobic bacteria for characterization host-microbe secreted metabolite interactions. This was designed compartmentalize anaerobes separate chambers conducive each...
Pneumonia is a world health problem and leading cause of death, particularly affecting children the elderly. Bacterial pneumonia following infection with influenza A virus (IAV) associated increased morbidity mortality but mechanisms behind this phenomenon are not yet well-defined. Host resistance tolerance processes essential for host survival during infection. Resistance host's ability to clear pathogen while overcome impact as well response Studies have shown that IAV suppresses immune...
Abstract Dietary composition and antibiotic use are known to have major impacts on the structure function of gut microbiome, often resulting in dysbiosis. Despite this, little research has been done explore role host diet as a determinant antibiotic-induced microbiome disruption. Here, we utilize multi-omic approach characterize impact Western-style consumption ciprofloxacin-induced changes community transcriptional activity. We found that mice consuming experienced greater expansion...
Effective gene transfer to CNS is key for development of new therapies neurological diseases. Relatively few natural AAV capsids cross the blood-brain barrier (BBB) upon systemic infusion and even then, transduction in adult brain after delivery limited mainly glia endothelium. Here we report tropism a vector generated by genetic grafting small peptide into capsid AAV9.47, liver-detargeted AAV9 mutant. The insertion was tolerated well with no loss packaging efficiency compared AAV9.47 or...
Therapy with the potent antifungal amphotericin B is significantly impacted by dose limiting toxicity to host cells. Thus, improve efficacy of this antifungal, we must understand molecular toxicology for both target fungi and mammalian host. Previous work has shown that fungicidal drugs, such as B, induce DNA damage in fungal cells, leading considerable cytotoxic effects. Therefore, modulating susceptibility cells may provide a mechanism fine‐tuning reducing side In NAD‐dependent histone...