A5064074509- Reproductive Biology and Fertility
- Reproductive Physiology in Livestock
- Virus-based gene therapy research
- Lysosomal Storage Disorders Research
- Sperm and Testicular Function
- Veterinary Medicine and Surgery
- Veterinary Equine Medical Research
- Veterinary Pharmacology and Anesthesia
- RNA Interference and Gene Delivery
- Thermal Regulation in Medicine
- RNA regulation and disease
- Sexual Differentiation and Disorders
- Assisted Reproductive Technology and Twin Pregnancy
- Animal Genetics and Reproduction
- Viral Infections and Immunology Research
- Herpesvirus Infections and Treatments
- Ovarian function and disorders
- Testicular diseases and treatments
- Retinal Development and Disorders
- Veterinary Oncology Research
- Cardiac Arrest and Resuscitation
- Diet and metabolism studies
- Renal and related cancers
- Trypanosoma species research and implications
- Reproductive System and Pregnancy
Auburn University
2015-2024
John Wiley & Sons (United States)
2020
Michigan State University
2015
University of Kentucky
2012
Texas A&M University
2010
Wellborn Road Veterinary Medical Center
2008
Adeno-associated viral (AAV) vectors have shown promise as a platform for gene therapy of neurological disorders. Achieving global delivery to the central nervous system (CNS) is key development effective therapies many these diseases. Here we report isolation novel CNS tropic AAV capsid, AAV-B1, after single round in vivo selection from an capsid library. Systemic injection AAV-B1 vector adult mice and cat resulted widespread transfer throughout with transduction multiple neuronal...
Effective gene delivery to the central nervous system (CNS) is vital for development of novel therapies neurological diseases. Adeno-associated virus (AAV) vectors have emerged as an effective platform in vivo transfer, but overall neuronal transduction efficiency derived from naturally occurring AAV capsids after systemic administration relatively low. Here, we investigated possibility improving CNS existing by genetically fusing peptides N-terminus VP2 capsid protein. A vector AAV-AS,...
Salutary responses to adeno-associated viral (AAV) gene therapy have been reported in the mouse model of Sandhoff disease (SD), a neurodegenerative lysosomal storage caused by deficiency β-N-acetylhexosaminidase (Hex). While untreated mice reach humane endpoint 4.1 months age, treated single intracranial injection vectors expressing human hexosaminidase may live normal life span 2 years. When with same therapeutic used mice, two cats SD lived 7.0 and 8.2 compared an 4.5 ± 0.5 (n = 11)....
Overweight and obesity are growing health problems in domestic cats, increasing the risks of insulin resistance, lipid dyscrasias, neoplasia, cardiovascular disease, decreasing longevity. The signature feline gut microbiota has not been studied at whole-genome metagenomic level. We performed shotgun sequencing fecal samples eight overweight/obese normal cats housed same research environment. obtained 271 Gbp sequences generated a 961-Mbp de novo reference contig assembly, with 1.14 million...
In a feline model of lysosomal storage disease, intracranial gene therapy achieved therapeutic efficacy in the CNS and increased long-term survival.
Sandhoff disease (SD) is an autosomal recessive neurodegenerative caused by a mutation in the gene for β-subunit of β-N-acetylhexosaminidase (Hex), resulting inability to catabolize ganglioside GM2 within lysosomes. SD presents with accumulation and its asialo derivative GA2, primarily central nervous system. Myelin-enriched glycolipids, cerebrosides sulfatides, are also decreased corresponding dysmyelination. At present, no treatment exists SD. Previous studies have shown therapeutic...
GM1 gangliosidosis is a fatal neurodegenerative disease that affects individuals of all ages. Favorable outcomes using adeno-associated viral (AAV) gene therapy in mice and cats have prompted consideration human clinical trials, yet there remains paucity objective biomarkers to track status. We developed panel blood, urine, cerebrospinal fluid (CSF), electrodiagnostics, 7 T MRI, magnetic resonance spectroscopy cats—either untreated or AAV treated for more than 5 years—and compared them...
Domestic dogs provide a unique challenge to the veterinary practitioner manipulate estrous cycle. Due an obligatory 120-day anestrus, cycle much less frequently than other domestic species. This can pose problem for owners and breeders wishing produce pups at certain part of year or temporarily prevent pregnancy due competition, health, convenience. We describe most common pharmacological methods available practitioners induction suppression canine with brief discussion on efficacy anecdotal...
It is our pleasure to bring you another special edition of Clinical Theriogenology. This focuses on Therapeutics in Theriogenology including estrus manipulation and diagnosis treatment selective ovarian diseases across species. issue includes eight review articles contributed by several authors. We thank colleagues for contributing this hope enjoy collection manuscripts.
Abstract Objective —To describe the health status of foals derived by use somatic cell nuclear transfer (NT) at a university laboratory. Design —Retrospective case series. Animals —14 live-born NT-derived foals. Procedures —Medical records from 2004 through 2008 were evaluated to identify all pregnancies resulting in Information obtained included gestation length, birth weight, foaling complications, gross abnormalities fetal membranes, appearance umbilicus, mentation foal, limb deformities,...
The endometrium, the inner uterine lining, is composed of cell layers that come in direct contact with an embryo during early pregnancy and later fetal placenta. endometrium responsible for signals associated normal reproductive cyclicity as well maintenance pregnancy. In mare, functionally competent vitro models have not been successful. Furthermore, ability to study various processes may allow critical evaluation signaling pathways involved diseases animals cannot be handled frequently,...
GM1 gangliosidosis is a fatal lysosomal disorder, for which there no effective treatment. Adeno-associated virus (AAV) gene therapy in cats has resulted greater than 6-fold increase lifespan, with many remaining alive at >5.7 years of age, minimal clinical signs. Glycolipids are the principal storage product whose pathogenic mechanism not completely understood. Targeted lipidomics analysis was performed to better define disease mechanisms and identify markers progression upcoming trials...
Arginine vasopressin (AVP) is used in human medicine the management of vasodilatory shock and cardiac arrest, but it not widely equine neonatal intensive care because concerns about potential side effects suboptimal efficacy. This retrospective study reports clinical use AVP norepinephrine (NE) foals with refractory hypotension.To report cardiovascular responses fluid balance critically ill, hypotensive receiving either NE or AVP.The medical records (<7 days age) from 2000 to 2007 admitted...
GM2 gangliosidosis is usually fatal by 5 years of age in its 2 major subtypes, Tay-Sachs and Sandhoff disease. First reported 1881, has no effective treatment today, children succumb to the disease after a protracted neurodegenerative course semi-vegetative state. This study seeks further develop adeno-associated virus (AAV) gene therapy for human translation.