A5087420269- Gut microbiota and health
- Cancer Research and Treatments
- Microbial Metabolic Engineering and Bioproduction
- Diet and metabolism studies
- Liver Disease Diagnosis and Treatment
- Adipose Tissue and Metabolism
- Metabolomics and Mass Spectrometry Studies
- Tissue Engineering and Regenerative Medicine
- Viral Infectious Diseases and Gene Expression in Insects
- Molecular Biology Techniques and Applications
- Bioinformatics and Genomic Networks
- 3D Printing in Biomedical Research
- Adipokines, Inflammation, and Metabolic Diseases
- Tryptophan and brain disorders
- Computational Drug Discovery Methods
- Clostridium difficile and Clostridium perfringens research
- Cancer, Hypoxia, and Metabolism
- Enzyme Catalysis and Immobilization
- Muscle metabolism and nutrition
- Biofuel production and bioconversion
- thermodynamics and calorimetric analyses
- Electrospun Nanofibers in Biomedical Applications
- Probiotics and Fermented Foods
- Gene Regulatory Network Analysis
- Bone health and treatments
Tufts University
2016-2025
Ministry of Health
2025
Texas A&M University
2014-2023
Texas A&M Health Science Center
2014-2023
Medford Radiology Group
2006-2023
Boston University
2016
Brigham and Women's Hospital
2014
Harvard University
1995-2014
Keimyung University
2014
USA Mitchell Cancer Institute
2014
The gut microbiota plays a significant role in the progression of fatty liver disease; however, mediators and their mechanisms remain to be elucidated. Comparing metabolite profile differences between germ-free conventionally raised mice against fed low- high-fat diet (HFD), we identified tryptamine indole-3-acetate (I3A) as metabolites that depend on are depleted under HFD. Both reduced fatty-acid- LPS-stimulated production pro-inflammatory cytokines macrophages inhibited migration cells...
Leptin is a protein encoded by the ob gene that expressed in adipocytes and regulates eating behavior via central neuroendocrine mechanisms. Serum leptin levels have been shown to correlate with weight percent body fat normal obese individuals; however, it not known whether regulation of below critical threshold chronic undernutrition. We investigated serum 22 women, aged 23 +/- 4 yr, anorexia nervosa. Duration disease, weight, BMI, fat, were determined for each patient. Nutritional status...
The tryptophan metabolites indole, indole-3-acetate, and tryptamine were identified in mouse cecal extracts fecal pellets by mass spectrometry. aryl hydrocarbon receptor (AHR) agonist antagonist activities of these microbiota-derived compounds investigated CaCo-2 intestinal cells as a model for understanding their interactions with colonic tissue, which is highly (Ah)–responsive. Activation Ah-responsive genes demonstrated that indole 3-acetate AHR agonists, whereas was an inhibited TCDD...
The non-invasive high-resolution spatial mapping of cell metabolism within tissues could provide substantial advancements in assessing the efficacy stem therapy and understanding tissue development. Here, using two-photon excited fluorescence microscopy, we elucidate relationships among endogenous fluorescence, redox state differentiation human mesenchymal cells into adipogenic osteoblastic lineages. Using liquid chromatography/mass spectrometry quantitative PCR, evaluate sensitivity an...
PTH-related peptide (PTHrP) is a causative agent of hypercalcemia associated with malignancy. PTHrP binds to and activates the same receptor as does PTH. Because has been suggested regulate growth differentiation cells paracrine/autocrine factor, we examined expression both its genes during fetal development rats (15-20 days gestation) by in situ hybridization riboprobes. Both messenger RNAs (mRNAs) were expressed not only skeleton, but also many extraskeletal tissues, such choroid plexus,...
To test the hypothesis that PTH-related peptide (PTHrP) is a paracrine regulator of endochondral bone development, we localized PTHrP and its cognate receptor during normal skeletal development at both messenger RNA (mRNA) protein levels compared growth plate phenotypes PTHrP-deficient [(PTHrP(-/-)] mice to those littermates [PTHrP(+/+]. mRNA was expressed adjacent uncavitated joints, in perichondrium long bones lower level proliferating chondrocytes. In contrast, most evident interface...
Significant osteoporosis affects over half of all women with anorexia nervosa (AN). The mechanisms bone loss in this condition are not known, and estrogen administration alone has been shown to prevent loss. Insulin-like growth factor I (IGF-I), a nutritionally dependent trophic hormone, is know stimulate osteoblast function collagen synthesis vivo vitro. We hypothesized that short term recombinant human IGF-I (rhIGF-I) would increase turnover young AN. studied 23 women, aged 18-29 yr (mean...
Modularity analysis is a powerful tool for studying the design of biological networks, offering potential clues relating biochemical function(s) network with 'wiring' its components. Relatively little work has been done to examine whether modularity depends on physiological perturbations that influence state. Here, we present novel algorithm based edge-betweenness centrality, which facilitates use directional information and measurable data.
The acquired immunodeficiency syndrome (AIDS) wasting (AWS) is a devastating complication of human virus infection characterized by disproportionate decrease in lean body mass. pathogenesis the AWS unknown, but recent data suggest that endogenous secretion potent anabolic hormone, testosterone; decreased 30-50% men with AIDS. However, it unknown whether androgen levels are associated mass and/or functional decreases muscle strength and aerobic capacity hypogonadal AWS. In addition,...
Alterations in the balance between different metabolic pathways used to meet cellular bioenergetic and biosynthetic demands are considered hallmarks of cancer. Optical imaging relying on endogenous fluorescence has been as a noninvasive approach assess tissue changes during cancer development. However, quantitative correlations optical assessments with variations concentration relevant metabolites or specific that involved have lacking. In this study, we use high-resolution, depth-resolved...
The endogenous ligand-activated aryl hydrocarbon receptor (AHR) plays an important role in numerous biologic processes. As the known number of AHR-mediated processes grows, so too does importance determining what AHR ligands are produced, how their production is regulated, and consequences ensue. Consequently, our studies were designed primarily to determine whether ER<sup>−</sup>/PR<sup>−</sup>/Her2<sup>−</sup> breast cancer cells have potential produce and, if so, these controlled. We...
Gut dysbiosis may play an etiological role in colorectal tumorigenesis. We previously observed that the abundance of Parabacteroides distasonis (Pd) stool was inversely associated with intestinal tumor burden and IL-1β concentrations mice. Here, we assessed anti-inflammatory capacity Pd membrane fraction (PdMb) colon cancer cell lines. In addition, tested whether could suppress tumorigenesis Six-week-old male A/J mice were fed a low-fat (LF) diet, high-fat (HF) diet or HF+ whole freeze-dried...
Despite excellent biocompatibility and mechanical properties, the poor in vitro vivo degradability of cellulose has limited its biomedical biomass conversion applications. To address this issue, we report a metabolic engineering-based approach to rational redesign cellular metabolites introduce N-acetylglucosamine (GlcNAc) residues into cellulosic biopolymers during de novo synthesis from Gluconacetobacter xylinus. The produced these engineered cells (modified bacterial [MBC]) was evaluated...
Several previous studies have pointed to environmental chemical exposure during windows of development as a contributing factor in neurodevelopmental disorders and correlated these with microbiota dysbiosis; however, little is known about how the chemicals specifically alter interfere development. The findings reported this paper unambiguously establish that pollutant linked can directly modify promote production potentially toxic metabolite ( p -cresol) has also been disorders. Furthermore,...