Gut dysbiosis may play an etiological role in colorectal tumorigenesis. We previously observed that the abundance of Parabacteroides distasonis (Pd) stool was inversely associated with intestinal tumor burden and IL-1β concentrations mice. Here, we assessed anti-inflammatory capacity Pd membrane fraction (PdMb) colon cancer cell lines. In addition, tested whether could suppress tumorigenesis Six-week-old male A/J mice were fed a low-fat (LF) diet, high-fat (HF) diet or HF+ whole freeze-dried...

Imbalance of the gut microbial community promotes inflammation and colorectal cancer (CRC). Previously, we demonstrated that freeze-dried Parabacteroides distasonis (Pd) suppressed obesity-driven tumorigenesis in mice. Here, investigated if Pd could suppress development colon tumors mice independent obesity. Six-week-old male A/J were assigned to receive: (i) chow diet (CTR); (ii) with 0.04% wt/wt (Pd-Early) or (iii) before switching (Pd-Late). Mice remained on for 25 weeks switch Pd-Late...

The aqueous extraction process of the leaves Rubus suavissimus often brings in a large amount nonactive polysaccharides as part constituents. To purify this water extract for potential elevated bioactivity, an alcohol precipitation (AP) consisting gradient regimens was applied, and its resultants were examined through colorimetric HPLC analyses. AP effective partitioning crude into soluble supernatant insoluble precipitant, effect varied significantly with regimens. Generally, higher...

The quality of botanical food is increasingly assessed by the content multiple bioactive compounds. This study reports, for first time, an HPLC fingerprinting method evaluation Rubus suavissimus leaves possessing bioactivities. Five constituents, gallic acid, rutin, ellagic rubusoside, and steviol monoside, were quantified used in developing qualitative chromatographic fingerprints. limits detection quantification ranged from 0.29 to 37.86 μg/mL. relative standard deviations (RSDs) intra-...

Drinking an herbal tea to lose weight is a well-liked concept. This study was designed examine the possible improvement of obesity phenotype by new represented its purified components, gallic acid, ellagic and rubusoside (GER). Male obese-prone SD rats were given low-fat diet, high-fat or diet plus GER at dose 0.22 g/kg body for 9 weeks. significantly reduced gain 22% compared control group with 48% less abdominal fat gain. Food intake not affected. Blood glucose lowered in GER-treated...

Paclitaxel (PTX) is one of the most potent intravenous chemotherapeutic agents to date, yet an oral formulation has been problematic because its low solubility and permeability. Using recently discovered solubilizing properties rubusoside (RUB), we investigated unique PTX-RUB formulation. PTX was solubilized by RUB in water levels 1.6-6.3 mg/ml at 10-40% weight/volume. These nanomicellar complexes were dried a powder, which subsequently reconstituted physiologic solutions. After 2.5 h,...

This study compares the inhibitory effects of orange peel polar fraction (OPP) and nonpolar (OPNP) on trimethylamine (TMA) N-oxide (TMAO) production in response to l-carnitine treatment vivo vitro. Metabolomics is used identify bioactive compounds. The research demonstrates that OPP effectively regulates atherosclerosis-related markers, TMA TMAO plasma urine, compared OPNP. Our investigation reveals these are independent changes gut microbiota composition. attributed modulation cntA/B enzyme...

High-fat diets (HFDs) and excess adiposity increase proinflammatory cytokines in the colon, altering gene expression a manner that promotes development of colorectal cancer (CRC). Thus, compounds reduce this biochemical inflammation are potential chemopreventive agents. Curcumin (CUR), dietary polyphenol, salsalate (SAL), non-steroidal anti-inflammatory drug, both anti-inflammatories. We investigated inhibitory effects CUR with or without SAL on inflammatory procarcinogenic signaling...

High-fat diets (HFDs) and adiposity increase colorectal cancer risk, in part by elevating pro-inflammatory cytokines that activate pro-cancerous signaling pathways. Curcumin (CUR), a dietary polyphenol salsalate (SAL), an non-steroidal anti-inflammatory drug (NSAID) lacking the gastrotoxicity of aspirin, each suppress inflammatory signaling, but via different cellular pathways.A/J mice (n = 110) are fed low-fat diet (LFD, 10% kcal), HFD (60% containing 0.4% CUR, 0.3% SAL, or both agents...

We previously demonstrated that feeding of dietary resistant starch (RS) prior to the induction diabetes delayed progression diabetic nephropathy and maintained vitamin D balance in streptozotocin (STZ)-induced type 1 (T1D) rats. Here, we examined impact RS on kidney function homeostasis following STZ injection. Male Sprague-Dawley rats were administered fed a standard diet containing cornstarch or 20, 10, 5% for 4 weeks. T1D 10 20% RS, but not gained more weight than cornstarch-fed Yet,...

Type 2 diabetes (T2D) is characterized by vitamin D deficiency owing to increased urinary loss of 25-hydroxycholecalciferol (25D). Whole eggs are a rich source D, particularly 25D, the circulating form that reflects status. Zucker diabetic (type 2) fatty (ZDF) rats and their lean counterparts were fed casein- or whole egg-based diets for 8 weeks. egg consumption attenuated both hyperglycemia hypertriglyceridemia, as well reduced weight gain in ZDF compared casein-fed rats. Circulating 25D...

The activation of the vitamin D receptor (VDR) in ileum has been shown to regulate Paneth cell-specific defensins, a large family antimicrobial peptides; hence, this may serve as potential mechanism maintain intestinal homeostasis. Previously, we have demonstrated that combination D3 (VD) and fructooligosaccharides (FOSs) upregulates colonic Vdr mice. Here, aim examine effect VD, alone or with FOSs, on barrier integrity secretion peptides, well gut microbial community. Male female C57BL/6J...

Background The importance of maternal nutrition to offspring health and risk disease is well established. Emerging evidence suggests paternal diet may affect as well. Objective In the current study we sought determine whether modulating pre-conception B vitamin intake alters intestinal tumor formation in offspring. Additionally, identify potential mechanisms for observed weight differential among by profiling hepatic gene expression lipid content. Methods Male Apc1638N mice (prone formation)...

A Western-style diet that is high in fat and sucrose has been shown to alter DNA methylation epigenetically modify genes related health risk offspring. Here, we investigated the effect of a methyl-donor nutrient (MS) supplemented high-fat, high-sucrose (HFS) during pregnancy lactation on vitamin D (VD) status inflammatory response After mating, 10-week-old female Sprague-Dawley (SD) rats (n = 10/group) were randomly assigned one four dietary groups lactation: (1) control (CON), (2) CON with...

Gut dysbiosis or imbalance of the gut microbial community promotes inflammation and contributes to pathogenesis several conditions such as aging, obesity, colorectal cancer (CRC). Previously, we demonstrated that feeding Parabacteroides distasonis (Pd) mice suppressed obesity-driven tumorigenesis. Here, investigated if Pd could suppress development progression colon tumors in independent obesity. Six-week old male A/J (n = 23/group) were randomly assigned one 3 diet regimens: 1) standard...

Obesity is a prominent risk factor for colorectal cancer (CRC). One mechanism by which obesity promotes the development of CRC generating chronic, low-grade state colonic inflammation. Interleukin-1β (IL-1β), proinflammatory cytokine often elevated in obesity, known to activate several procarcinogenic signaling pathways that are implicated carcinogenesis. We therefore sought define role IL-1β mediating some early biochemical and molecular events leading up obesity-promoted CRC. Twenty-five...