A5025709365- Cervical Cancer and HPV Research
- Genital Health and Disease
- Hepatitis B Virus Studies
- Reproductive tract infections research
- Adolescent Sexual and Reproductive Health
- Female Genital Mutilation/Cutting Issues
- Gut microbiota and health
- Syphilis Diagnosis and Treatment
- Hepatitis Viruses Studies and Epidemiology
- Global Cancer Incidence and Screening
- Molecular Biology Techniques and Applications
- Head and Neck Cancer Studies
- Urinary Tract Infections Management
- HIV Research and Treatment
- HIV/AIDS oral health manifestations
- Colorectal and Anal Carcinomas
- Reproductive System and Pregnancy
- T-cell and Retrovirus Studies
- Ethics in Clinical Research
- AI in cancer detection
- Animal Virus Infections Studies
- Urological Disorders and Treatments
- Sexual Assault and Victimization Studies
- Cancer Risks and Factors
- Pelvic floor disorders treatments
National Health Laboratory Service
2016-2025
University of Cape Town
2015-2024
Nelson Mandela Academic Hospital
2020-2024
South African Medical Research Council
2019-2024
Walter Sisulu University
2020-2024
MRC Cancer Unit
2022
Groote Schuur Hospital
2009-2020
National Institute for Communicable Diseases
2020
Wellcome Centre for Infectious Diseases Research in Africa
2010
Background. This study investigated genital human papillomavirus (HPV) incidence and clearance in 278 immunodeficiency virus (HIV)–seropositive (HIV-positive) women, 208 HIV-negative 161 HIV-positive men, 325 followed at 6-month intervals for up to 24 months.
Both cervical cancer and human immunodeficiency virus (HIV) are major public health problems in Sub-Saharan Africa. The objectives of the study were to investigate papillomavirus (HPV) prevalence according age, HIV status gender.Participants 208 HIV-negative women, 278 HIV-positive 325 men 161 between ages 18-66 years. HPV types determined penile cells by Roche Linear Array genotyping assay.HPV was 36.7 % (76/207; 95 confidence intervals (CI): 30.4-43.4 %) with highest 61.0 (25/41; CI:...
The objectives of the study were to investigate prevalence cervical human papillomavirus (HPV) genotypes inform HPV vaccination strategy in South Africa and factors associated with prevalence. Sexually active, HIV-negative women, aged 16–22 years recruited from Soweto (n = 143) Cape Town 148) tested for other genital infections. Overall was 66.7% (194/291) young women. women more likely have multiple infections than (48.0%, 71/148 versus 35.0%, 50/143 respectively, p 0.033) probable HR-HPV...
This study examined the concordance of genital human papillomavirus (HPV) infection in 254 heterosexually active couples and impact HIV coinfection. Genital HPV detection was significantly more common among HIV-infected women than HIV-seronegative (99 [68%] 145 vs. 33 [31%] 107 women; P < .001); similarly, men (67 [72%] 93 65 [43%] 150 men, respectively; .001). male partners had a greater prevalence did (38 [58%] 27 [32%] 85 men; = .001), indicating that coinfection one partner has...
Adolescents in Africa are at high risk for HIV infection, other sexually transmitted infections (STIs) and bacterial vaginosis (BV). Since behavior burden of STIs/BV may influence risk, behavioral factors prevalence were compared HIV-seronegative adolescent females (n = 298; 16–22 years) from two South African communities (Soweto Cape Town). STIs ( Chlamydia trachomatis, Neisseria gonorrhoeae, Trichomonas vaginalis, Mycoplasma genitalium, herpes simplex virus (HSV)-1, HSV-2, Treponema...
ObjectivesTo investigate the prevalence of high-risk (HR) human papillomavirus (HPV) and factors associated with HR-HPV infection among women from rural Eastern Cape, South Africa.MethodsHPV was determined by Hybrid Capture 2 assay in cervical specimens 417 aged ≥30 years (median 46 years) recruited community health clinic Cape.ResultsHR-HPV 28.5% (119/417), HIV-positive had significantly higher than HIV-negative (40.6%, 63/155 vs 21.4%, 56/262, respectively; p = 0.001). status (odds ratio...
In this study we examined potential associations of HPV infection with the cervical microbiota. Cervical samples were collected from 87 HIV-seronegative reproductive-age Black South African women. Microbiota characterized by Illumina sequencing V3-V4 hypervariable regions bacterial 16S rRNA gene. Thirty seven (42.5%) and 30 (34.5%) women had prevalent high-risk (HR)-HPV, respectively. Only 23 (26.4%) microbiota dominated a single Lactobacillus species (L. crispatus (2/87 (2.3%)), L. jensenii...
Human papillomavirus (HPV) prevalence and genotype distribution data is important for HPV vaccine monitoring. This study investigated the of genotypes in cervical lesions unvaccinated women referred to Nelson Mandela Academic Hospital Gynaecology Department due different abnormal conditions. A total 459 department were recruited. When biopsy was collected histopathology, an adjacent provided detection. Roche Linear Array genotyping assay that detects 37 used detect infection biopsies....
ABSTRACT Objectives Human papillomavirus (HPV) is the leading cause of cervical cancer, with adolescent girls and young women (AGYW) in sub-Saharan Africa carrying a disproportionately high burden infection. Hormonal contraceptives may influence HPV acquisition, persistence, clearance, but evidence remains inconclusive. This sub-study aimed to evaluate impact different hormonal on prevalence genotype distribution AGYW. Methods Ninety-eight HIV-seronegative AGYW aged 15–19 years from South...
Objectives HPV infection causes cervical cancer, yet information on prevalence and risk factors for in Africa remain sparse. This study describes the of genotypes associated with among young women ≤ 30 years age KwaZulu-Natal (KZN), South Africa. Methods Cervicovaginal lavage samples were tested 224 enrolled a prospective cohort study. Clinical, behavioural demographic data collected. We measured using logistic regression, examined HPV. Results Median participants was 21 [interquartile range...
Adolescent girls and young women represent a key risk group for sexually transmitted infections (STIs). The vaginal microbiota is thought to play an important role in susceptibility STIs such as Chlamydia trachomatis. We compared the of lateral wall endocervix, assessed associations with C. trachomatis infection South African adolescents. endocervical were characterized by amplifying sequencing V4 region 16S rRNA gene diagnosed using molecular methods. Of 72 included, 30 had asymptomatic...
Abstract Background To date, the microbiota of human penis has been studied mostly in connection with circumcision, HIV risk and female partner bacterial vaginosis (BV). These studies have shown that male circumcision reduces penile anaerobic bacteria, greater abundance bacteria is correlated increased cytokine levels infection, an important harbour for BV-associated bacteria. While to significantly reduce acquiring papillomavirus (HPV) relationship HPV still unknown. In this study, we...
South African women of reproductive age have a high burden sexually transmitted infections (STIs), including human papillomavirus (HPV) infection. However, there is limited information on the prevalence pathogens in from rural Eastern Cape Province, Africa. The study aims at determining and co-infection with high-risk (HR) HPV among Africa.A total 205 cervical specimens were collected aged ≥ 30 years community-based clinic. samples tested for panel pathogenic STIs [Chlamydia trachomatis...
This study investigated the impact of human immunodeficiency virus (HIV) infection on genital papillomavirus (HPV) in heterosexual couples. More HIV-positive men and women had HPV compared with HIV-negative (77 vs 49 %; P<0.001) (74 36 P<0.001). partners who were positive those HPV-negative (for men, 72 % 40 Men female at greater risk high-risk low-risk (LR HPV) partners. increased decreasing CD4 count {≥350 ml−1: odds ratio [OR ], 2.37 [95 confidence interval (CI), 1.47–3.83]; <350 OR, 3.02...
As part of the human papillomavirus (HPV) vaccination strategy in South Africa, it is essential to have information on HPV prevalence, and types distribution among unvaccinated population. Information prevalence adolescents young women Africa's Eastern Cape Province minimal. Therefore, this study investigates types, factors associated with infection amongst female learners. A sample composed 213 sexually active learners attending high schools Africa; median age 18 years, who provided...
Abstract Infections by HIV increase the risk of acquiring secondary viral and bacterial infections methods are needed to determine spectrum co-infections for proper treatment. We used rolling circle amplification (RCA) Ion Proton sequencing investigate vaginal microbiome 20 positive women from South Africa. A total 46 different human papillomavirus (HPV) types were found, many which not detected existing genotyping assays. Moreover, complete genomes two novel HPV determined. Abundance was...
Lactobacillus spp. are common bacteria in the cervical and vaginal microbiota (CVM) thought to represent a "healthy" cervicovaginal state. Several studies have found an independent association between ethnicity/race composition. Women of sub-Saharan African descent appear be significantly more likely non-Lactobacillus-dominated CVM compared women European descent. The factors contributing these differences remain fully elucidated. Black South influencing their understudied. In this study, we...
Abstract Human papillomavirus (HPV) testing is highly sensitive compared to cytology, with the trade‐off of being less specific. We investigated whether select combinations HPV genotypes, ascertained by Linear Array (LA) and Xpert (GX), can optimize sensitivity/specificity trade‐offs detect high‐grade cervical intraepithelial neoplasia (CIN2+). In a study in Cape Town, South Africa, 586 women living without 535 HIV, aged 30‐65 years, were recruited. Each woman underwent pelvic exam collect...
South African women have a high rate of cervical cancer cases, but there are limited data on human papillomavirus (HPV) genotypes in intraepithelial neoplasia (CIN) the Eastern Cape province, Africa. A total 193 specimens with confirmed CIN from aged 18 years or older, recruited referral hospital, were tested for HPV infection. The specimens, smeared onto FTA cards, screened 36 types using an direct flow kit. prevalence was 93.5% (43/46) CIN2 and 96.6% (142/147) CIN3. HIV-positive had...
Vaccines against human papillomavirus (HPV) are the key to controlling cervical cancer in low/middle-income countries (LMICs) where incidence is highest, but there have been limited data from these settings on programme impact HPV prevalence, and none a population with endemic HIV infection. Furthermore, for many LMICs, currently recommended two-dose schedule difficult deliver at scale, so mounting interest single-dose schedule.
Cervical cancer is more common in Sub-Saharan Africa (SSA) compared to developed countries, with persistent genital high-risk HPV (HR-HPV) infection identified as the cause. However, other factors contributing this gap remain unclear. This review explores potential role of cervicovaginal microbiota (CVM) and cervical development among women SSA. Many African descent, including those from SSA, lack lactobacilli dominance their CVM, which considered a biomarker health. Published literature has...
This study investigated the performance of Cepheid Xpert human papillomavirus (HPV) assay in South African immunodeficiency virus (HIV)-infected women and compared its with that hybrid capture-2 (hc2). Methods: Stored cervical specimens from HIV-infected had previously been tested using hc2 were Xpert. Results: The overall HR-HPV prevalence was found to be 62.0% (720/1161) by 61.2% (711/1161) hc2. 13.6% (158/1161) HPV16 positive, 18.8% (218/1161) HPV18/45, 37.3% (434/1161) HPV31/33/35/52/58,...