A5033649718- Acute Myeloid Leukemia Research
- Protein Degradation and Inhibitors
- Hematopoietic Stem Cell Transplantation
- Cancer-related molecular mechanisms research
- Multiple Myeloma Research and Treatments
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- MicroRNA in disease regulation
- Histone Deacetylase Inhibitors Research
- Mesenchymal stem cell research
- Reproductive System and Pregnancy
- Rabies epidemiology and control
- RNA Research and Splicing
- Microbial infections and disease research
- Cancer-related gene regulation
- RNA Interference and Gene Delivery
- Extracellular vesicles in disease
- Orthopedic Infections and Treatments
- Clinical Laboratory Practices and Quality Control
- Immune Cell Function and Interaction
- Bacterial Identification and Susceptibility Testing
- RNA modifications and cancer
- Virus-based gene therapy research
- Veterinary Orthopedics and Neurology
- Parasitic Infections and Diagnostics
- Toxoplasma gondii Research Studies
The Ohio State University
2015-2022
University of Wisconsin–Madison
2009-2022
American College of Veterinary Pathologists
2022
Long non-coding RNAs (lncRNAs) are important regulatory molecules that implicated in cellular physiology and pathology. In this work, we dissect the functional role of HOXB-AS3 lncRNA patients with NPM1-mutated (NPM1mut) acute myeloid leukemia (AML). We show regulates proliferative capacity NPM1mut AML blasts vitro vivo. is shown to interact ErbB3-binding protein 1 (EBP1) guide EBP1 ribosomal DNA locus. Via mechanism, RNA transcription de novo synthesis. propose context NPM1 mutations,...
Abstract Acute graft-versus-host disease (aGVHD) continues to be a frequent and devastating complication of allogeneic hematopoietic stem cell transplantation (HSCT), posing as significant barrier against the widespread use HSCTs curative modality. Recent studies suggested serum/plasma microRNAs (miRs) may predict aGVHD onset. However, little is known about functional role circulating miRs in aGVHD. In this article, we show two independent cohorts that miR-29a expression significantly...
Acute graft-versus-host disease (aGVHD) is a T cell–mediated immunological disorder and the leading cause of nonrelapse mortality in patients who receive allogeneic hematopoietic cell transplants. Based on recent observations that protein arginine methyltransferase 5 (PRMT5) methylation are upregulated activated memory cells, we hypothesized PRMT5 involved pathogenesis aGVHD. Here, show expression enzymatic activity were cells vitro from mice developing aGVHD after transplant. was also...
Significance In this work we report on the previously uncharacterized clinical and biological role for EGFL7 in acute myeloid leukemia (AML). Patients with increased mRNA expression had lower complete remission rates shorter overall event-free survival, demonstrating relevance of cytogenetically normal AML. Our results show that AML blasts are able to synthesize secrete protein, promoting autocrine blast cell growth. Inhibition decreased proliferation induces apoptosis cells. Taken together,...
Abstract MicroRNA-155 (miR-155) is a small noncoding RNA critical for the regulation of inflammation as well innate and adaptive immune responses. MiR-155 has been shown to be dysregulated in both donor recipient cells during acute graft-versus-host disease (aGVHD). We previously reported that miR-155 upregulated T mice humans with aGVHD receiving miR-155–deficient (miR155−/−) splenocytes had markedly reduced aGVHD. However, molecular mechanisms by which modulates cell function have not...
EGF-like domain 7 (EGFL7) is a secreted protein and recently has been shown to play an important role in acute myeloid leukemia (AML); however, the underlying mechanism by which EGFL7 promotes leukemogenesis largely unknown.
Acute graft- versus -host disease (GVHD) is the leading cause of non-relapse mortality following allogeneic hematopoietic cell transplantation. The majority patients non-responsive to front line treatment with steroids have an estimated overall 2-year survival rate only 10%. Bromodomain and extra-terminal domain (BET) proteins influence inflammatory gene transcription, therefore represent a potential target mitigate inflammation central acute GVHD pathogenesis. Using potent selective BET...
Abstract A 4‐year‐old male neutered domestic shorthair cat was presented to The Ohio State University College of Veterinary Medicine for a 2‐month history severe weight loss, lethargy, anemia, and bilaterally hyperechoic kidneys with loss corticomedullary distinction as reported by the referring veterinarian. Relevant initial laboratory results included non‐regenerative normocytic hypochromic increased blood urea nitrogen, minimally concentrated urine, proteinuria, an urine...
Abstract An 8‐year‐old, 6‐kg, male neutered Domestic Shorthair cat was presented to The Ohio State University Veterinary Medical Center ( OSU ‐ VMC ) for difficulty breathing. Physical examination and thoracic radiographs indicated pneumonia, a soft‐tissue mass in the left caudal lung lobe, diffuse pleural effusion. effusion classified as modified transudate. Rare extracellular elongated (~5–7 μm × 1–2 μm) zoites with central round oval‐shaped purple deep vesicular nucleus coarsely stippled...
Abstract Introduction T cell mediated acute graft-versus-host disease (aGVHD) is the main cause of non-relapse mortality in allogeneic hematopoietic transplant recipients. Protein arginine methyltransferase 5 (PRMT5) a post-translational modifier and promotes activation memory TH cells. We investigate mechanisms by which PRMT5 regulates function propose inhibition as therapeutic strategy for aGVHD. Materials Methods expression was evaluated cells healthy aGVHD mice humans. assessed...
We reported that miR-155 expression is upregulated in donor T cells during aGVHD and mice receiving knock-out (KO) splenocytes do not exhibit lethal GVHD have improved survival as compared to wild type (WT) splenocytes1. Here, we investigate the impact of cell migration elucidate population responsible for mediated modulation aGVHD.
<p>Additional Supplementary Data</p>
<div>AbstractPurpose:<p>EGF-like domain 7 (EGFL7) is a secreted protein and recently has been shown to play an important role in acute myeloid leukemia (AML); however, the underlying mechanism by which EGFL7 promotes leukemogenesis largely unknown.</p>Experimental Design:<p>Using antibody interaction array, we measured ability of bind directly approximately 400 proteins expressed primary AML blasts. Primary patient samples were stimulated <i>in vitro</i>...
<div>AbstractPurpose:<p>EGF-like domain 7 (EGFL7) is a secreted protein and recently has been shown to play an important role in acute myeloid leukemia (AML); however, the underlying mechanism by which EGFL7 promotes leukemogenesis largely unknown.</p>Experimental Design:<p>Using antibody interaction array, we measured ability of bind directly approximately 400 proteins expressed primary AML blasts. Primary patient samples were stimulated <i>in vitro</i>...
<p>Additional Supplementary Data</p>
Background: Acute Graft-Versus-Host Disease (aGVHD) continues to be a frequent and lethal complication of allogeneic hematopoietic cell transplantation (allo-HCT), highlighting the urgent need develop therapeutic strategies prevent, mitigate or delay aGVHD. The bromodomain extra-terminal domain (BET) family proteins regulate activity oncogenes as well oncogenic fusion in leukemia; have emerged novel targets for intervention hematological malignancies. In addition their potential, BET also...
Abstract Background: A HOXB-locus-embedded lncRNA, named HOXB-AS3 significantly associates with NPM1 mutations in AML. Herein, we evaluate the functional role of expression NPM1mut Methods: was measured by real-time PCR. Knock-down (KD) performed vitro and vivo locked nucleic acid-modified gapmers. RNA antisense purification (RAP), RNA-immunoprecipitation (RIP), Chromatin-immunoprecipitation (ChIP) experiments were according to published protocols. Results: Among 7 AML cell lines tested,...