A5068222952- Pain Mechanisms and Treatments
- Musculoskeletal pain and rehabilitation
- Ion Channels and Receptors
- Pain Management and Treatment
- Nerve injury and regeneration
- Chemical and Physical Studies
- Medical Imaging and Analysis
- Burn Injury Management and Outcomes
- Biochemical effects in animals
- Cannabis and Cannabinoid Research
- Mitochondrial Function and Pathology
- Biofield Effects and Biophysics
- Dermatologic Treatments and Research
- Neuropeptides and Animal Physiology
- Cellular Mechanics and Interactions
- Neuroscience and Neuropharmacology Research
- Traumatic Brain Injury Research
- Spine and Intervertebral Disc Pathology
- Neurobiology and Insect Physiology Research
- Laser Applications in Dentistry and Medicine
- Hemoglobinopathies and Related Disorders
- Traumatic Brain Injury and Neurovascular Disturbances
- Adenosine and Purinergic Signaling
- Veterinary Pharmacology and Anesthesia
- Erythrocyte Function and Pathophysiology
Medical College of Wisconsin
2020-2024
Tactile and spontaneous pains are poorly managed symptoms of inflammatory neuropathic injury. Here, we found that transient receptor potential canonical 5 (TRPC5) is a chief contributor to both these sensations in multiple rodent pain models. Use TRPC5 knockout mice inhibitors revealed selectively contributes the mechanical hypersensitivity associated with CFA injection, skin incision, chemotherapy induced peripheral neuropathy, sickle cell disease, migraine, all which were characterized by...
Abstract Increased excitability of primary sensory neurons after peripheral nerve injury may cause hyperalgesia and allodynia. Dorsal root ganglion field stimulation (GFS) is effective in relieving clinical pain associated with neuropathic animal models. However, its mechanism has not been determined. We examined effects GFS on transmission action potentials (APs) from the to central processes by vivo single-unit recording lumbar dorsal roots sham injured rats tibial (TNI) fiber types...
Many patients with chronic pain conditions suffer from depression. The mechanisms underlying pain-induced depression are still unclear. There critical links of medial prefrontal cortex (mPFC) synaptic function to depression, signaling through the endocannabinoid (eCB) system as an important contributor. We hypothesized that afferent noxious inputs after injury compromise activity-dependent eCB in mPFC, resulting Depression-like behaviors were tested male and female rats traumatic neuropathy...
Few analgesics identified using preclinical models have successfully translated to clinical use. These translational limitations may be due the unidimensional nature of behavioral response measures used assess rodent nociception. Advances in high-speed videography for pain behavior allow objective quantification nuanced aspects evoked paw withdrawal responses. However, whether videography-based assessments mechanical hypersensitivity outperform traditional measurement reproducibility is...
A major hurdle preventing effective interventions for patients with mild traumatic brain injury (mTBI) is the lack of known mechanisms long-term cognitive impairment that follows mTBI. The closed head impact model repeated engineered rotational acceleration (rCHIMERA), a non-surgical animal mTBI (rmTBI), mimics key features rmTBI in humans. Using rCHIMERA rats, this study was designed to characterize rmTBI-induced behavioral disruption, underlying electrophysiological changes medial...
Abstract Dorsal root ganglion field stimulation (GFS) relieves evoked and spontaneous neuropathic pain by use-dependent blockade of impulse trains through the sensory neuron T-junction, which becomes complete within less than 1 minute for C-type units, also with partial Aδ units. We used this tool in spinal nerve ligation (SNL) rat model to selectively block activity (SA) axotomized neurons at fifth lumbar (L5) level vs units L4 that remain uninjured but exposed inflammation. In vivo dorsal...
Abstract Background Intervertebral disc degeneration (IVDD) is a primary cause of degenerative diseases; however, the mechanisms underlying remain unclear. The immunoinflammatory response plays an important role in IVDD progression. inflammatory cytokine lymphotoxin-α (LTα), formerly known as TNFβ, associated with various pathological conditions, while its pathogenesis remains elusive. Methods Real-time quantitative polymerase chain reaction (RT-qPCR), Western blotting (WB), and...
Abstract Persistent tactile pain is a poorly managed symptom of inflammatory and neuropathic injury. To develop therapies for this maladaptive sensation, the underlying molecular mediators must be identified. Using knockout mice pharmacological inhibitors, we identified transient receptor canonical 5 (TRPC5) as key contributor to persistent that occurs in many preclinical rodent models. TRPC5 inhibition was effective injuries associated with elevated levels bioactive phospholipid...