A5035973434- Cancer, Hypoxia, and Metabolism
- Diet and metabolism studies
- Pain Mechanisms and Treatments
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Fibroblast Growth Factor Research
- Ion Transport and Channel Regulation
- Cancer Cells and Metastasis
- Metabolism, Diabetes, and Cancer
- Cancer Research and Treatments
- Electrolyte and hormonal disorders
- Botulinum Toxin and Related Neurological Disorders
- Lung Cancer Treatments and Mutations
- Genetic Neurodegenerative Diseases
- Cancer-related Molecular Pathways
- Nerve injury and regeneration
- Parkinson's Disease Mechanisms and Treatments
- Adipose Tissue and Metabolism
- Neurobiology and Insect Physiology Research
- Immune cells in cancer
- Bee Products Chemical Analysis
- Erythrocyte Function and Pathophysiology
- Veterinary Pharmacology and Anesthesia
- Mitochondrial Function and Pathology
- Liver physiology and pathology
University of Kansas Medical Center
2017-2025
Medical College of Wisconsin
2024-2025
Institute of Cell Biology and Neurobiology
2021-2024
University Medical Center
2018-2022
University Hospital and Clinics
2018
Despite aggressive therapies, head and neck squamous cell carcinoma (HNSCC) is associated with a less than 50% 5-year survival rate. Late-stage HNSCC frequently consists of up to 80% cancer-associated fibroblasts (CAF). We previously reported that CAF-secreted HGF facilitates progression; however, very little known about the role CAFs in metabolism. Here, we demonstrate increases extracellular lactate levels via upregulation glycolysis. induced basic FGF (bFGF) secretion from HNSCC. were...
Two-thirds of patients with Fabry disease suffer debilitating pain attacks triggered by exercise, fever, and exposure to environmental heat. These face endure even greater risk heat-related episodic in the global climate change. Almost nothing is known about biological mechanisms underlying heat-induced crises disease, there no preclinical model available for study crises. Here, we established first exposing transgenic rats Heat precipitated robust mechanical hypersensitivity, closely...
Few analgesics identified using preclinical models have successfully translated to clinical use. These translational limitations may be due the unidimensional nature of behavioral response measures used assess rodent nociception. Advances in high-speed videography for pain behavior allow objective quantification nuanced aspects evoked paw withdrawal responses. However, whether videography-based assessments mechanical hypersensitivity outperform traditional measurement reproducibility is...
Patients with Fabry disease suffer from chronic debilitating pain and peripheral sensory neuropathy minimal treatment options, but the cellular drivers of this are unknown. Here, we propose a mechanism believe to be novel in which altered signaling between Schwann cells neurons underlies nerve dysfunction observed genetic rat model disease. Using vivo vitro electrophysiological recordings, demonstrated that exhibited pronounced hyperexcitability. probably contributed finding because...
Abstract We previously reported that ionizing radiation (IR) mediates cell death through the induction of CUGBP elav‐like family member 2 (CELF2), a tumor suppressor. CELF2 is an RNA binding protein modulates mRNA stability and translation. Since IR induces autophagy, we hypothesized regulates autophagy‐mediated colorectal cancer (CRC) death. For clinical relevance, determined levels in The Cancer Genome Atlas (TCGA). Role response was carried out CRC lines by immunoblotting,...
Objectives/Hypothesis Human papillomavirus (HPV) is a DNA virus that causes cancer in multiple sites. Although sexual activity the primary means of oropharyngeal HPV acquisition, studies suggest transmission through occupational exposure from medical instruments and surgical fumes. We assess if aerosolization HPV16 via electrocautery places otolaryngologists at risk for exposure. Study Design Animal human laboratory model. Methods Plasmid (pLXSN16E6E7) expressing p16 E6/E7 genes was...
Dietary interventions are promising approaches to treat pain associated with metabolic changes because they impact both and neural components contributing painful neuropathy. Here, we tested whether consumption of a ketogenic diet could affect sensation, pain, epidermal innervation loss in type 1 diabetic mice. C57Bl/6 mice were rendered using streptozotocin administered at either 3 weeks (prevention) or 9 (reversal) uncontrolled diabetes. We quantified biomarkers, sensory thresholds, assess...
Methylglyoxal (MGO) is a reactive dicarbonyl byproduct of glycolysis implicated in growing number neuropathic pain conditions, including chemotherapy-induced peripheral neuropathy, diabetic and radiculopathy with lumbar disk herniation. Recent studies show success preclinical models treating these disorders an interventional ketogenic diet. Here, we tested the hypothesis that diet modifies pathological MGO signaling as mechanism underlying neuropathy improvement. We found mice injected...
Chronic pain is a substantial health burden and options for treating chronic remain minimally effective. Ketogenic diets are emerging as well-tolerated, effective therapeutic strategies in preclinical models of pain, especially diabetic neuropathy. We tested whether ketogenic diet antinociceptive through ketone oxidation related activation ATP-gated potassium (K
Chronic pain is a substantial health burden and options for treating chronic remain minimally effective. Ketogenic diets are emerging as well-tolerated, effective therapeutic strategies in preclinical models of pain, especially diabetic neuropathy. We tested whether ketogenic diet antinociceptive through ketone oxidation related activation ATP-gated potassium (KATP) channels mice. demonstrate that consumption one week reduced evoked nocifensive behaviors (licking, biting, lifting) following...
Ketogenic diets are emerging as protective interventions in preclinical and clinical models of somatosensory nervous system disorders. Additionally, dysregulation succinyl-CoA 3-oxoacid CoA-transferase 1 (SCOT, encoded by
<p>CAF secreted HGF mediates HNSCC glycolysis.</p>
<p>HNSCC Cells have Higher Glycolytic Capacity than CAFs and Maximal Respiration HNSCC.</p>
<p>Combination of AZD-4547 and PF-02341066 indicates potentiated effect in admixed cultures but not HNSCC only inoculated tumors.</p>