Abstract We report the most extensive genetic linkage map for a livestock species produced to date. have linked 376 microsatellite (MS) loci with seven restriction fragment length polymorphic in backcross reference population. The 383 markers were placed into 24 groups which span 1997 cM. Seven additional MS did not fall group. Linkage are assigned 13 autosomes and X chromosome (haploid n = 19). This provides basis analysis of quantitative inheritance phenotypic physiologic traits swine.

We have developed a panel of 152 whole-genome radiation hybrids by fusing irradiated diploid pig lymphocytes or fibroblasts with recipient hamster permanent cells. The number and size the porcine chromosome fragments retained in each hybrid clone were checked fluorescence situ hybridization SINE probe primed labeling (PRINS) SINE-specific primers. A strategy based on interspersed repetitive sequence polymerase chain reaction (IRS-PCR) was for selected clones to determine if large painted...

In postpartum beef cows, GnRH-induced ovulation of small dominant follicles decreased pregnancy rates and increased late embryonic/fetal mortality. Exp. 1, single reciprocal embryo transfer (ET) was used to examine the relationship between preovulatory serum concentrations estradiol at in donor recipient cows establishment maintenance pregnancy. Suckled (n = 1,164) were administered GnRH (GnRH1, 100 μg) on d -9 (GnRH1), PGF(2α) -2, GnRH2 (GnRH2, 0 (CO-Synch protocol) either with (donors; n...

A single ovulation, reciprocal embryo transfer study was used to investigate effects of oocyte competence and maternal environment on pregnancy establishment maintenance in beef cows. Estrous cycles were synchronized suckled cows donors inseminated d 0 (n = 810). Cows classified as having a small (<12.5 mm) or large (≥12.5 ovulatory follicle randomly chosen recipients remove confounding size fertility. Embryos 393) oocytes 44) recovered 7, all viable embryos transferred into 354). All...

We report the sequences, sizes, and number of alleles 414 new porcine microsatellites that were cloned in our laboratory 21 derived from GenBank DNA sequences. also confirm usefulness microsatellite primer pairs short interdispersed elements.

The first integrated physical and genetic linkage map encompassing the entire swine chromosome 7 (SSC7) reveals that porcine MHC (SLA) spans centromere. A SLA class II antigen gene lies on q arm, whereas I III genes lie p suggesting presence of a centromere within does not preclude functional complex. appears smaller than other mammalian MHC, as distance across two I, three II, markers is only 1.1 cM. There are significant variations in recombination rates function position along chromosome,...

Journal Article Complete sequence of the bovine β-lactoglobulin cDNA Get access L.J. Alexander, Alexander School Biochemistry, University New South WalesP0 Box 1, Kensington, NSW 2033, Australia Search for other works by this author on: Oxford Academic PubMed Google Scholar G. Hayes, Hayes M.J. Pearse, Pearse A.F. Stewart, Stewart I.M. Willis, Willis A.G. Mackinlay * *To whom correspondence should be addressed Nucleic Acids Research, Volume 17, Issue 16, 25 August 1989, Page 6739,...

Genomics is a rapidly evolving field of research that provides radical approaches to understanding the links between genetic code and diseases. Significant breakthroughs in last decade have now positioned animal health community capitalize on infrastructure built by human genome project. Major milestones include draft sequences for several species, including chicken, dog, cattle, horse, while sequencing cat, pig, sheep genomes has been initiated.This volume presents proceedings international...

Extensive range livestock production systems in the western United States rely heavily on rangeland forages to meet nutritional needs of grazing throughout year. Interannual variation quantity and quality forage Northern Great Plains, as well much States, may play a pivotal role how ruminants sequester nutrients their tissues. This influence ability beef cow utilize dietary via changes tissue responsiveness insulin. Identifying specific periods states which this phenomenon is manifested will...

Although various attempts have been made to isolate a submitochondrial fraction capable of oxidative phosphorylation, it was not until the recent work Cooper and Lehninger (1) that any degree success achieved. Among techniques used for disrupting mitochondria ultrasonic disintegration, freezing-thawing, dispersion with variety chemical agents, variations in pH values. slight phosphorylation (low P:O) sometimes achieved (a), such treatment preserved only portion system. recently obtained...

A whole-genome scan was conducted on 328 F(2) progeny in a Wagyu x Limousin cross to identify quantitative trait loci (QTL) affecting palatability and fatty acid composition of beef at an age-constant endpoint. We have identified seven QTL five chromosomes involved lipid metabolism tenderness. None the genes encoding major enzymes metabolism, such as synthase (FASN), acetyl-CoA carboxylase alpha (ACACA), solute carrier family 2 (facilitated glucose transporter) member 4 (SLC2A4),...

An autosomal scan of the swine genome with 119 polymorphic microsatellite (ms) markers and data from 116 F2 barrows University Illinois Meishan x Yorkshire Swine Resource Families identified genomic regions effects on variance in carcass composition meat quality at nominal significance (p-value <0.05). Marker intervals chromosomes 1, 6, 7, 8 12 (SSC1, SSC6, SSC7, SSC8, SSC12) phenotypic length, 10th rib backfat thickness, average leaf fat, loin eye area intramuscular fat content confirm QTL...

Direct in situ single-copy polymerase chain reaction (DISC-PCR) was used to assign and orient a linkage group pig chromosome 1. Five microsatellites were analyzed, all five successfully localized using this procedure. Physical data the with respect centromere estimate amount of coverage There excellent concordance between physical maps. The linear order identical, relative distances similar. All markers located on long arm Coverage estimated at about 32%. Thus, DISC-PCR rapidly easily...

A microsatellite-based genome scan of a Wagyu x Limousin F(2) cross population previously demonstrated QTL affecting LM area and fatty acid composition were present in regions near the centromere BTA2. In this study, we used 70 SNP markers to examine centromeric 24 megabases (Mb) BTA2, including Limousin-specific F94L myostatin allele (AB076403.1; 415C > A) located at approximately 6 Mb on draft sequence significant effect marker was observed (F = 60.17) for area, which indicated that is...

A complex of geophysical methods were used to investigate a small karst area aimed at the production detailed geological mapping, confirm localization known sinkholes, and find possible continuations caves voids below surface.The dipole electromagnetic profiling radiometric mapping (the gamma-ray spectrometry method) applied determine spatial distribution hard carbonate rocks weathered valley-fill sediments.Detailed high-definition magnetometry was carried out selected sites in studied...

We describe a conceptual model for genome databases that facilitates the process of building, maintaining, and disseminating physically anchored genetic linkage maps. The has been implemented as relational database at Roman L. Hruska U.S. Meat Animal Research Center (MARC). Development consensus maps using disparate data from different reference pedigrees or laboratories is supported. use to quantitative population geneticists interested in loci affect phenotypes marker-assisted selection,...

Summary. Porcine cDNAS clones encoding β‐casein were isolated and sequenced. The porcine cDNA is 1100bp in length, excluding the poly (A) tail, encodes a preprotein of 232 amino acids.

The objectives of this study were to assign both microsatellite and gene‐based markers on porcine chromosome X two radiation hybrid (RH) panels develop a more extensive integrated map SSC‐X. Thirty‐five 20 assigned T43RH, 16 previously unreported 15 added IMpRH map. Of these, 30 12 common RH maps. Twenty‐two submitted BLASTN analysis for identification orthologues genes HSA‐X. Single nucleotide polymorphisms (SNPs) detected markers, nine these placed the genetic A total 92 known loci are...