A5062595170- Advanced biosensing and bioanalysis techniques
- Gold and Silver Nanoparticles Synthesis and Applications
- Nanocluster Synthesis and Applications
- Prion Diseases and Protein Misfolding
- Parkinson's Disease Mechanisms and Treatments
- Alzheimer's disease research and treatments
- Force Microscopy Techniques and Applications
- RNA Interference and Gene Delivery
- RNA and protein synthesis mechanisms
- Advanced Fluorescence Microscopy Techniques
- Advanced Nanomaterials in Catalysis
- Biosensors and Analytical Detection
- Cellular transport and secretion
- Nanopore and Nanochannel Transport Studies
- Conducting polymers and applications
- Biotin and Related Studies
- Carbon and Quantum Dots Applications
- Proteins in Food Systems
- RNA modifications and cancer
- Neurological disorders and treatments
- Lipid Membrane Structure and Behavior
- RNA Research and Splicing
- Enzyme Structure and Function
- Polymer Surface Interaction Studies
- Nanotechnology research and applications
University of Nebraska at Omaha
2016-2025
University of Nebraska Medical Center
2006-2020
Seattle Pacific University
2019
University of California, Santa Barbara
2019
Technical University of Munich
2019
Aarhus University
2019
Boston University
2016
Tufts University
2016
Universidad Nacional Autónoma de México
2016
Instituto Nacional de Seguridad y Salud en el Trabajo
2016
Adipose-derived mesenchymal stem/stromal cells (ADMSC) are one of the major stromal in breast cancer microenvironment that promote progression. Previous studies on effects ADMSC metastasis and drug resistance, using two-dimensional (2D) cultures, remained inconclusive. In present study, we compared cocultured human epidermal receptor 2 positive primary (21PT) 2D three-dimensional (3D) cultures then examined their response to doxorubicin (DOX). We 3D bioprinted constructs with middle edge...
Abstract With the rapid iteration of portable electronics and electric vehicles, developing high-capacity batteries with ultra-fast charging capability has become a holy grail. Here we report rechargeable aluminum-ion capable reaching high specific capacity 200 mAh g −1 . When liquid metal is further used to lower energy barrier from anode, fastest rate 10 4 C (duration 0.35 s reach full capacity) 500% more under high-rate conditions are achieved. Phase boundaries active anode believed...
Nanomaterials have been extensively explored in developing sensors due to their unique properties, contributing the development of reliable sensor designs with improved sensitivity and specificity. Herein, we propose construction a fluorescent/electrochemical dual-mode self-powered biosensor for advanced biosensing using DNA-templated silver nanoclusters (AgNCs@DNA). AgNC@DNA, its small size, exhibits advantageous characteristics as an optical probe. We investigated sensing efficacy...
Alpha-synuclein (α-Syn) is a 140 aa presynaptic protein which belongs to group of natively unfolded proteins that are unstructured in aqueous solutions. The aggregation rate α-Syn accelerated the presence physiological levels cellular polyamines. Here we applied single molecule AFM force spectroscopy characterize effect spermidine on very first stages – misfolding and assembly into dimers. Two variants, wild-type (WT) A30P, were studied. two molecules covalently immobilized at C-terminus,...
The blood–brain barrier (BBB) is an active and complex diffusion that separates the circulating blood from brain extracellular fluid, regulates nutrient transportation, provides protection against various toxic compounds pathogens. Creating in vitro microphysiological BBB system, particularly with relevant human cell types, will significantly facilitate research of neuropharmaceutical drug delivery, screening, transport, as well improve our understanding pathologies are due to damage....
RNA is an attractive biopolymer for engineering self-assembling materials suitable biomedical applications. Previously, programmable hexameric rings were developed the controlled delivery of up to six different functionalities. To increase potential functionalization with little impact on nanoparticle topology, we introduce gaps into double-stranded regions rings. Molecular dynamic simulations are used assess behavior and changes in flexibility novel designs. The suggested by simulations,...
Cells maintain spatiotemporal control over biochemical processes through the formation and dissolution of biomolecular condensates, dynamic membraneless organelles formed via liquid–liquid phase separation. Composed primarily proteins nucleic acids, these condensates regulate key cellular functions, their properties are influenced by concentration type molecules involved. The structural versatility challenges de novo design assembly with predefined properties. Through feedback between...
MicroRNAs (miRNAs) are small, non-coding RNAs that play critical roles in regulating gene expression and implicated various diseases, including cancer, cardiovascular disorders, neurodegenerative diseases. Due to their diagnostic prognostic significance, the development of sensitive, specific, reliable detection methods for miRNAs has become a research priority. Nucleic-acid-based approaches offer unique advantages, high specificity, potential amplification, adaptability platforms. This...
Self-assembly of misfolded proteins into ordered fibrillar structures is a fundamental property wide range and peptides. This also linked with the development various neurodegenerative diseases such as Alzheimer's Parkinson's. Environmental conditions modulate misfolding aggregation processes. We used peptide, CGNNQQNY, from yeast prion protein Sup35, model system to address effects environmental on aggregate formation. The GNNQQNY peptide self-assembles in fibrils structural features that...
Environmental factors, such as acidic pH, facilitate the assembly of α-synuclein (α-Syn) in aggregates, but impact pH on very first step α-Syn aggregation remains elusive. Recently, we developed a single-molecule approach that enabled us to measure directly stability dimers. Unlabeled monomers were immobilized substrate, and fluorophore-labeled added solution allow them form dimers with monomers. The dimer lifetimes measured from fluorescence bursts time trajectories. Herein, applied...
Silver has a long history of antibacterial effectiveness. The combination atomically precise metal nanoclusters with the field nucleic acid nanotechnology given rise to DNA-templated silver (DNA-AgNCs) which can be engineered reproducible and unique fluorescent properties activity. Furthermore, cytosine-rich single-stranded DNA oligonucleotides designed fold into hairpin structures improve stability AgNCs additionally modulate their quality observed signals. In this work, we characterize...
Combining atomically resolved DNA-templated silver nanoclusters (AgNCs) with nucleic acid nanotechnology opens new exciting possibilities for engineering bioinorganic nanomaterials uniquely tunable properties.
Besides being a passive carrier of genetic information, DNA can also serve as an architecture template for the synthesis novel fluorescent nanomaterials that are arranged in highly organized network functional entities such silver nanoclusters (AgNCs). Only few atoms size, properties AgNCs be tuned using variety templating sequences, overhangs, and neighboring duplex regions. In this study, we explore manufactured on short sequence-an individual element designed construction larger DNA-based...
DNA-templated silver nanoclusters (AgNC@DNA) are a novel type of nanomaterial with advantageous optical properties. Only few atoms in size, the fluorescence can be tuned using DNA overhangs. In this study, we explored properties AgNCs manufactured on short single-stranded (dC)12 when adjacent G-rich sequences (dGN , N = 3-15) were added. The 'red' emission AgNC@dC12 λMAX 660 nm dramatically changed upon addition overhang NG 15. pattern emission-excitation matrix (EEM) suggested emergence two...
Micro RNA (miR) are regulatory non-coding molecules, which contain a small number of nucleotides ~18–28 nt. There many various miR sequences found in plants and animals that perform important functions developmental, metabolic, disease processes. miRs can bind to complementary within mRNA molecules thus silencing mRNA. Other include cardiovascular neural development, stem cell differentiation, apoptosis, tumors. In tumors, some function as oncogenes, others tumor suppressors. Levels certain...