A5026263190- Plant tissue culture and regeneration
- Geochemistry and Geologic Mapping
- Transgenic Plants and Applications
- Plant Molecular Biology Research
- CRISPR and Genetic Engineering
- Plant Reproductive Biology
- Hydrocarbon exploration and reservoir analysis
- Photosynthetic Processes and Mechanisms
- Radioactive element chemistry and processing
- Plant Virus Research Studies
- Legume Nitrogen Fixing Symbiosis
- Soybean genetics and cultivation
- Geological and Geochemical Analysis
- Bioenergy crop production and management
- Nematode management and characterization studies
- Geoscience and Mining Technology
- Geochemistry and Geochronology of Asian Mineral Deposits
- Plant biochemistry and biosynthesis
- Plant nutrient uptake and metabolism
- Biofuel production and bioconversion
- Light effects on plants
- Plant Stress Responses and Tolerance
- Plant Taxonomy and Phylogenetics
- Plant Pathogens and Fungal Diseases
- Forest, Soil, and Plant Ecology in China
North Carolina State University
2018-2025
Beijing Research Institute of Uranium Geology
2012-2024
National Institute for Radiological Protection
2022-2023
Beltsville Agricultural Research Center
2022
U.S. National Arboretum
2022
University of Tennessee at Knoxville
2005-2021
Xinyu University
2021
Nanchang University
2021
Tongji University
2008
Beijing Forestry University
1999
Chloroplast DNA sequences are a primary source of data for plant molecular systematic studies. A few key papers have provided the systematics community with universal primer pairs noncoding regions that dominated field, namely trnL-trnF and trnK/matK. These two adequate information to resolve species relationships in some taxa, but often provide little resolution at low taxonomic levels. To obtain better phylogenetic resolution, sequence from these coupled other data. Choosing an appropriate...
Abstract Computational tool-assisted primer design for real-time reverse transcription (RT) PCR (qPCR) analysis largely ignores the sequence similarities between sequences of homologous genes in a plant genome. It can lead to false confidence quality designed primers, which sometimes results skipping optimization steps qPCR. However, qPCR parameters plays an essential role efficiency, specificity, and sensitivity each gene’s primers. Here, we proposed optimized approach sequentially...
Abstract Plant transformation and regeneration remain highly species- genotype-dependent. Conventional hormone-based plant via somatic embryogenesis or organogenesis is tedious, time-consuming, requires specialized skills experience. Over the last 40 years, significant advances have been made to elucidate molecular mechanisms underlying organogenesis. These pioneering studies led a better understanding of key steps factors involved in regeneration, resulting identification crucial growth...
Summary Salicylic acid plays a critical role in activating plant defence responses after pathogen attack. methyltransferase (SAMT) modulates the level of salicylic by converting to methyl salicylate. Here, we report that SAMT gene from soybean ( GmSAMT1 ) against cyst nematode Heterodera glycines Ichinohe, SCN). was identified as candidate SCN defence‐related our previous analysis using GeneChip microarray experiments. The current study started with isolation full‐length cDNA s SCN‐resistant...
The ubiquitin protein is present in all eukaryotic cells and promoters from genes are good candidates to regulate the constitutive expression of transgenes plants. Therefore, two switchgrass (Panicum virgatum L.) (PvUbi1 PvUbi2) were cloned characterized. Reporter constructs produced containing isolated 5' upstream regulatory regions coding sequences (i.e. PvUbi1 PvUbi2 promoters) fused uidA region (GUS) tested for transient stable a variety plant species tissues. consists 607 bp cis-acting...
Uranium, as one of twenty–six kinds important minerals in China, is strategic resource and energy mineral, which has been thoroughly investigated the project National Potential Evaluation Uranium Resource. During implementation this project, authors summarized metallogenic regularity uranium China systematically, through researches geological characteristics resource, deposits type (genetic prediction type), temporal spatial distribution, series. Based on investigation present situation...
Abiotic stress resistance traits may be especially crucial for sustainable production of bioenergy tree crops. Here, we show the performance a set rationally designed osmotic-related and salt stress-inducible synthetic promoters use in hybrid poplar. De novo motif-detecting algorithms yielded 30 water-deficit (SD) 34 (SS) candidate DNA motifs from relevant poplar transcriptomes. We selected three conserved (SD18, SD13 SD9) found 16 co-expressed gene promoters, discovered well-conserved motif...
Lettuce genetic transformation is genotype-dependent. In the present study, we have successfully developed an optimized Agrobacterium-mediated protocol for elite lettuce cultivars, which belong to romaine, leaf, and butterhead cultivar types. We type concentration of plant growth regulators (PGRs) selection antibiotics found that use 1-naphthaleneacetic acid (NAA; 0.10 mg/L) 6-benzyladenine (BA; 0.25 as regulators, hygromycin (15 transgenic selection, cotyledons first true leaf explants...
We aimed to engineer transgenic plants for the purpose of early detection plant pathogen infection, which was accomplished by employing synthetic inducible promoters fused reporter genes altered phenotypes in response infection. Toward this end, a number consisting regulatory elements red fluorescent protein (RFP) were constructed use phytosensing.For rapid analysis, an Agrobacterium-mediated transient expression assay evaluated, then utilized assess inducibility each promoter construct...
Gene expression in eukaryotes is often enhanced by the presence of introns. Depending on specific gene, this enhancement can be minor or very large and occurs at both transcriptional post-transcriptional levels. The Arabidopsis ERECTA gene contains 27 exons encoding a receptor-like kinase that promotes cell proliferation inhibits differentiation above-ground plant organs. strongly depends intronless does not rescue phenotype erecta mutant plants produces about 500–900 times less protein...
Summary Computational methods offer great hope but limited accuracy in the prediction of functional cis ‐regulatory elements; improvements are needed to enable synthetic promoter design. We applied an ensemble strategy for de novo soybean cyst nematode ( SCN )‐inducible motif discovery among promoters 18 co‐expressed genes that were selected from six reported microarray studies involving a compatible soybean– interaction. A total 116 overlapping regions OMR s) discovered bioinformatically...
SUMMARY Drought stress triggers the accumulation of phytohormone abscisic acid (ABA), which in turn activates expression floral integrator gene CONSTANS ( CO ), accelerating flowering. However, molecular mechanism ABA‐induced activation remains elusive. Here, we conducted a yeast one‐hybrid assay using promoter from Brassica campestris (syn. rapa ) ssp. chinensis (pak choi) to screen pak choi library and identified transcription activator ABF3 (BrABF3). BrABF3, was induced by ABA choi,...
Abstract Compared with most flowers where the showy part comprises specialized leaves (petals) directly subtending reproductive structures, Zingiberaceae species produce “flowers” through modifications of (bracts) true throughout an inflorescence. Curcuma alismatifolia , belonging to family, a plant originating from Southeast Asia, has become increasingly popular in flower market worldwide because its varied and esthetically pleasing bracts produced different cultivars. Here, we present...
A method using Agrobacterium rhizogenes-mediated callus production and plant regeneration enables efficient genetic transformation gene editing in Chinese cabbage.
Plants are subject to attack by a wide range of phytopathogens. Current pathogen detection methods and technologies largely constrained those occurring post-symptomatically. Recent efforts were made generate plant sentinels (phytosensors) that can be used for sensing reporting contamination in crops. Engineered phytosensors indicating the presence pathogens as early-warning potentially have tremendous utility wide-area detectors. We previously showed synthetic promoters containing and/or...
Leafy head formation in Chinese cabbage (B. rapa ssp. pekinensis cv. Bre) results from leaf curvature, which is under the tight control of genes involved adaxial-abaxial patterning during development. The transcriptional coactivator ANGUSTIFOLIA3 (AN3) binds to SWI/SNF chromatin remodeling complexes formed around ATPases such as BRAHMA (BRM) order regulate transcription various aspects development cell proliferation, primordia expansion, and adaxial/abaxial Arabidopsis. However, its...