A5106630935- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- Nail Diseases and Treatments
- Inhalation and Respiratory Drug Delivery
- Antimicrobial Peptides and Activities
- Asthma and respiratory diseases
- Allergic Rhinitis and Sensitization
- Glioma Diagnosis and Treatment
- Helminth infection and control
- Herbal Medicine Research Studies
- Fungal Biology and Applications
- Respiratory and Cough-Related Research
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Histone Deacetylase Inhibitors Research
- Hair Growth and Disorders
- IL-33, ST2, and ILC Pathways
- Genetics, Aging, and Longevity in Model Organisms
- Mathematical Biology Tumor Growth
- Cannabis and Cannabinoid Research
- Peptidase Inhibition and Analysis
- Dermatology and Skin Diseases
- Cancer, Hypoxia, and Metabolism
- Lichen and fungal ecology
- Mycorrhizal Fungi and Plant Interactions
- HIV/AIDS drug development and treatment
California State Polytechnic University
2006-2022
Gilead Sciences (United States)
2013-2018
University of Oklahoma
2007
Pennsylvania State University
2007
Cornell University
2006
Memorial Sloan Kettering Cancer Center
2006
University of New Orleans
1986
Tulane Medical Center
1986
National Institute of Allergy and Infectious Diseases
1986
National Institutes of Health
1986
The fungal cell wall is a critically important structure that represents permeability barrier and protective shield. We probed Candida albicans Cryptococcus neoformans with liposomes containing amphotericin B (AmBisome), or without 15-nm colloidal gold particles. have diameter of 60 to 80 nm, yet their mode action requires them penetrate the deliver membrane, where it binds ergosterol. Surprisingly, using cryofixation techniques electron microscopy, we observed remained intact during transit...
ABSTRACT While Candida albicans remains the most common isolate, glabrata accounts for approximately 15 to 20% of all infections in United States. In this study we used immunosuppressed mice infected with C. investigate efficacy liposomal amphotericin B alone or combination echinocandin caspofungin micafungin. For monotherapy, were given six daily doses (3 20 mg/kg body weight), (1 5 mg/kg), micafungin (2.5 10 mg/kg). With concomitant therapy, received (7.5 mg/kg) addition 6 days. sequential...
Invasive aspergillosis, an important cause of morbidity and mortality in immunosuppressed (IS) patients, is often treated with amphotericin B lipid formulations. In the present study, liposomal (L-AMB) complex (ABLC) were compared treatment murine pulmonary aspergillosis. Uninfected, IS mice for 4 days 1, 4, 8, or 12 mg L-AMB ABLC/kg body weight, their lungs analyzed by high-performance liquid chromatography drug concentrations. intranasally challenged Aspergillus fumigatus 12, 15, 20 mg/kg...
ABSTRACT Small unilamellar amphotericin B liposomes can reduce the toxicity of B. In this study, we compared physical, antifungal, pharmocokinetic, and toxic properties two liposomal products, AmBisome Anfogen, that have same chemical composition but are manufactured differently. vitro tests included determinations MICs concentrations causing release 50% intracellular potassium from red blood cells (K 50 values) to assess toxicity. The lethal dose (LD ) was evaluated by using uninfected...
We hypothesized that effective prophylactic treatment of fungal infections would require adequate drug penetration and retention at potential infection sites. Using a mouse model, we examined liposomal amphotericin B (L-AmB) biodistribution, cell localization in kidneys, lungs, liver spleen to evaluate dosing regimens for prophylaxis Candida glabrata albicans infections.Following mice with cumulative doses L-AmB (60-225 mg/kg), bioassay was done determine tissue concentrations 12 h 6 weeks...
Because of the reduced toxicity associated with liposomal amphotericin B preparations, different liposome products have been made. In present study, we compared formulations, AmBisome® (AmBi) and Lambin® (Lbn), in uninfected Aspergillus fumigatus infected mice, using several vitro vivo efficacy assays. The results showed that formulations were significantly different, Lbn 1.6-fold larger than AmBi. was also more toxic AmBi based on RBC potassium release assay intravenous dosing mice given a...
ABSTRACT Monotherapy and combination therapy were compared using optimal doses of liposomal amphotericin B, micafungin, or caspofungin in Aspergillus fumigatus pulmonary disseminated infections. Mice challenged intravenously (2.8 × 10 4 to 5.7 conidia) intranasally (5.8 7 with A. . Drugs (5, 10, 15 mg/kg body weight) given for 3 6 days as single, concomitant, sequential (i.e., 1 then 6). monitored survival, tissues assayed fungal burden drug concentrations. Treatments starting 24 h...
This study was done to determine whether high dose AmBisome (4-20 mg/kg), given intermittently, could reduce the frequency of dosing needed treat murine systemic candidiasis when compared with conventional daily treatment.Mice were immunosuppressed cyclophosphamide every 3 days, beginning day -3 before challenge log(10) 5.0 cfu Candida albicans. Treatment begun 48-72 h post-challenge or intermittent regimens AmBisome, followed by determination kidney for up 1 month post-treatment.A single 4...
Amphotericin B formulations were compared in preclinical models by using intraperitoneal (ip) and intravenous (iv) delivery of amphotericin deoxycholate (DAMB) or liposomal B. We examined the effects on drug tissue penetration retention resulting from different routes administration. Mice treated with equivalent total doses AmBisome (AmBi) DAMB (i.e.,15 mg/kg) given ip (3 mg/kg/day for 5 days) iv AmBi days 1 15 days), tissues collected 24 h post-treatment. For studies, mice 30 mg/kg (10 3 x...
Given the recent increase in aspergillosis caused by species other than Aspergillus fumigatus, micafungin, caspofungin, and liposomal amphotericin B (L-AmBi) were investigated as monotherapy or combination therapy for murine systemic pulmonary flavus infection. Treatment 3 6 days was begun at 24 h (intravenous [i.v.], 2.8 × 10(4) conidia) 2 (intranasal, 4.1 10(6) to 6.75 postchallenge follows: 5 10 mg/kg L-AmBi, 15 L-AmBi plus echinocandin, on 1 echinocandin 4 6, 6. Mice monitored survival,...
Delta-9-tetrahydrocannabinol (Δ9-THC), the psychoactive component of marijuana, is known to suppress immune responses bacterial, viral and protozoan infections, but its effects on fungal infections have not been studied. Therefore, we investigated chronic Δ9-THC treatment mouse resistance systemic Candida albicans (C. albicans) infection. To determine outcome primary, acute candidiasis, c57BL/6 mice were given vehicle or (16 mg/kg) in days 1–4, 8–11 15–18. On day 19, infected with 5×105 C....
Candida species are the 4th leading cause of nosocomial infections in US affecting both men and women. Since males many can be more susceptible to than females, we investigated whether male mice were systemic albicans (C. albicans) infection if sex hormones responsible for sex-dependent susceptibility this infection. Non-gonadectomized or gonadectomized supplemented with sustained release 5α-dihydrotestosterone (5αDHT) 17-β-estradiol (E2) using subcutaneous pellet implantation. Mice...
Skin prick test activity and antigenicity of extracts in vitro growth the Basidiomycete Pleurotus ostreatus (PO) were compared to spores from PO growing wild. Patients demonstrated significant differences skin reactivity extracts. Some reacted only extracts, others spore 1 patient all Further studies analyzed antigens present with rabbit antisera PO. Common as well unique those preparations. The fact that contain suggests basidiospores may be best source relevant allergens for clinical studies.
Abstract Background Antifungal treatment for pulmonary aspergillosis is more difficult if the fungal strain causing infection azole resistant. To investigate this problem, we used a murine model of caused by azole-resistant Aspergillus fumigatus strains V29 and V45, compared with voriconazole (Vr, oral 40 mg/kg, bid) or liposomal amphotericin B (L-AmB, 5 IV) alone in combination. Methods Mice (n = 14/gp) were immunosuppressed 24 mg/kg triamcinolone acetonide, IP, d-3, d-1, d+1 relative to...
Due to ethical and budgetary concerns associated with the use of vertebrate animals in research, interest alternative models has increased over past several decades. In present study, we developed a Candida albicans quantitative infection model Caenorhabditis elegans, nonparasitic invertebrate nematode, test antifungal effects liposomal amphotericin B (L-AmB). To establish lethal C. infection, larval Stage 4 worms [n = 30/group (gp)] were fed various doses yeast (2.5 × 102–2.5 105 cells/gp)...
Abstract Background Immunocompromised patients are very susceptible to pulmonary aspergillosis causing 50% mortality with present treatments, indicating a need for improved therapy. To address this, we standardized nebulization method effectively delivering liposomal amphotericin B (AmBisome®, AmBi) into lungs of Aspergillus fumigatus-infected mice. Methods AmBi particle characterization was done Cascade impactor and Schuco S5000 nebulizer containing 1.33 mg/mL AmBi. For in vivo studies,...