A5057047768- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Escherichia coli research studies
- Genetic and phenotypic traits in livestock
- RNA and protein synthesis mechanisms
- Adipose Tissue and Metabolism
- Microbial infections and disease research
- CAR-T cell therapy research
- RNA Research and Splicing
- Genetic Mapping and Diversity in Plants and Animals
- Adipokines, Inflammation, and Metabolic Diseases
- Bacterial Genetics and Biotechnology
- Epigenetics and DNA Methylation
- Circular RNAs in diseases
- Virus-based gene therapy research
- Cytokine Signaling Pathways and Interactions
- Cardiovascular Disease and Adiposity
- Viral Infectious Diseases and Gene Expression in Insects
- Peroxisome Proliferator-Activated Receptors
- Genetic Associations and Epidemiology
- Menopause: Health Impacts and Treatments
- Mycobacterium research and diagnosis
- Regulation of Appetite and Obesity
- Helminth infection and control
- Cancer-related gene regulation
In JeT ApS (Denmark)
2024
University of Copenhagen
2013-2022
Obesity is a complex condition that increases the risk of life threatening diseases such as cardiovascular disease and diabetes. Studying gene regulation obesity important for understanding molecular mechanisms behind derived may lead to better intervention treatment plans. MicroRNAs (miRNAs) are short non-coding RNAs regulating target mRNA by binding their 3'UTR. They involved in numerous biological processes diseases, including obesity. In this study we use mixed breed pig model designed...
The pig is a well-known animal model used to investigate genetic and mechanistic aspects of human disease biology. They are particularly useful in the context obesity metabolic diseases because other widely models (e.g. mice) do not completely recapitulate key pathophysiological features associated with these humans. Therefore, we established F2 resource population (n = 564) designed elucidate genetics underlying phenotypes. Segregation traits was ensured by using breeds highly divergent...
Obesity is a rising worldwide public health problem. Difficulties to precisely measure various obesity traits and the genetic heterogeneity in human have been major impediments completely disentangle factors causing obesity. The pig relevant model for studying obesity-related (OOR) traits. Using founder breeds divergent with respect we created an F2 resource population (454 pigs), which has intensively phenotyped 36 OOR main rationale our study characterize architecture of design, by...
Obesity is associated with immunological perturbations that contribute to insulin resistance. Epigenetic mechanisms can control immune functions and have been linked metabolic complications, although their contribution resistance still remains unclear. In this study, we investigated the link between dysfunction alterations epigenetic signature in leukocytes a porcine model of obesity. Global DNA methylation circulating leukocytes, adipose tissue leukocyte trafficking, macrophage polarisation...
Infection of the small intestine by enterotoxigenic Escherichia coli F4ab/ac is a major welfare problem and financial burden for pig industry. Natural resistance to this infection inherited as Mendelian recessive trait, polymorphism in MUC4 gene segregating susceptibility/resistance presently used selection programme Danish breeding To elucidate genetic background involved E. susceptibility pigs, detailed haplotype map porcine candidate region was established. This covers approximately 3.7...
Enterotoxigenic Escherichia coli (ETEC) that express the F4ab and F4ac fimbriae is a major contributor to diarrhoea outbreaks in pig breeding industry, infecting both newborn weaned piglets. Some pigs are resistant this infection, susceptibility inherited as simple dominant Mendelian trait. Indentifying genetics behind trait will greatly benefit welfare well industry by providing an opportunity select against genetically susceptible animals, thereby reducing number of outbreaks. The has...
Obesity and its comorbidities are an increasing challenge for both affected individuals health care systems, worldwide. In obese individuals, perturbation of expression protein-coding genes microRNAs (miRNA) seen in obesity-relevant tissues (i.e. adipose tissue, liver skeletal muscle). miRNAs small non-coding RNA molecules which have important regulatory roles a wide range biological processes, including obesity. Rodents widely used animal models human diseases However, not all research is...
Reprogramming of adipocyte function in obesity is implicated metabolic disorders like type 2 diabetes. Here, we used the pig, an animal model sharing many physiological and pathophysiological similarities with humans, to perform in-depth epigenomic transcriptomic characterization pure fractions. Using a combined DNA methylation capture sequencing Reduced Representation bisulfite (RRBS) strategy 11 lean 12 obese pigs, identified 3529 differentially methylated regions (DMRs) located at close...
Abstract Background Model animals are valuable resources for dissecting basic aspects of the regulation obesity and metabolism. The translatability results relies on understanding comparative molecular pathophysiology. Several studies have shown that despite presence overt dyslipidemia in pig key human pathological hepatic findings such as hepatocellular ballooning abundant steatosis lacking model. Objectives aim this study was to elucidate why these histopathological characteristics did not...
Taste receptors (TASRs) and appetite reward (AR) mechanisms influence eating behaviour, which in turn affects food intake risk of obesity. In a previous study, we used next generation sequencing to identify potentially functional mutations TASR AR genes found indications for genetic associations between identified variants growth fat deposition subgroup animals (n = 38) from the UNIK resource pig population. This population was created studying obesity obesity-related diseases. present study...
Abstract The contribution of microRNAs (miRNAs) to mRNA post‐transcriptional regulation has often been explored by the post hoc selection downregulated genes and determining whether they harbor binding sites for miRNAs interest. This approach, however, does not discriminate these mRNAs are also at transcriptional level. Here, we have characterized changes in expression two porcine tissues: gluteus medius muscle fasted fed Duroc gilts adipose tissue lean obese Duroc–Göttingen minipigs....
Abstract Cellular effector function assays traditionally rely on bulk cell populations that mask complex heterogeneity and rare subpopulations. The Xdrop® droplet technology facilitates high-throughput compartmentalization of viable single cells or single-cell pairs in double-emulsion droplets, enabling the study cell-cell interactions at an individual level. Effector molecule secretion target killing can be evaluated independently combination. Compatibility with a wide range commercial...
<h3>Background</h3> Heterogeneity in cellular functions such as cytokine secretion and cytotoxicity are often overlooked when assessing the potency of cell therapy products due to lack suitable assay technologies. Further, current assays detect these functionalities cannot be combined must performed parallel experiments. Single-cell resolution is partially achieved by intracellular staining (ICS), but this requires protein transport inhibitors, which may introduce artifacts. Additionally,...
Abstract The contribution of microRNAs (miRNAs) to mRNA regulation has often been explored by post hoc selection downregulated genes and determining whether they harbor binding sites for miRNAs interest. This approach, however, does not discriminate these mRNAs are also at the transcriptional level. Here, we have characterized post-transcriptional changes expression in two porcine tissues: gluteus medius muscle fasted fed Duroc gilts adipose tissue lean obese Duroc-Göttingen minipigs....