A5060128772- Adipose Tissue and Metabolism
- Diet and metabolism studies
- Regulation of Appetite and Obesity
- Cardiovascular Disease and Adiposity
- Adipokines, Inflammation, and Metabolic Diseases
- Gut microbiota and health
- Diet, Metabolism, and Disease
- Liver Disease Diagnosis and Treatment
- Atherosclerosis and Cardiovascular Diseases
- Migraine and Headache Studies
- Pharmacological Effects and Assays
- Receptor Mechanisms and Signaling
- Coronary Interventions and Diagnostics
- Neuropeptides and Animal Physiology
- Metabolism, Diabetes, and Cancer
- Ophthalmology and Eye Disorders
- Cardiovascular Health and Disease Prevention
- Cardiac Imaging and Diagnostics
- Peroxisome Proliferator-Activated Receptors
- Dietary Effects on Health
- Statistical Methods in Clinical Trials
- Infrared Thermography in Medicine
- Animal health and immunology
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Glaucoma and retinal disorders
Novo Nordisk (Denmark)
2015-2025
Novo Nordisk (United Kingdom)
2021
University of Copenhagen
2013
Royal Agricultural University
2006
Danish Diabetes Association
2006
Oslo University Hospital
1978
Background Recent evidence suggests that the gut microbiota is an important contributing factor to obesity and related metabolic disorders, known as syndrome. The aim of this study was characterise intestinal in two pig models namely Göttingen minipigs Ossabaw minipigs. Methods Findings cecal, ileal colonic from lean obese Osabaw were investigated by Illumina-based sequencing high throughput qPCR, targeting 16S rRNA gene different phylogenetic groups bacteria. weight gain through significant...
The objectives were 1) to characterize a Göttingen Minipig model of metabolic syndrome regarding its colon microbiota and circulating microbial products, 2) assess whether ovariectomized female castrated male minipigs show similar phenotypes. Twenty-four nine-week-old Minipigs allocated four groups based on sex diet: females males fed either chow or high-fat diet (HFD) for 12 weeks. At study end, body composition plasma biomarkers measured, mixed meal tolerance test (MMT) an intravenous...
The importance of the gut microbiota (GM) in disease development has recently received increased attention, and numerous approaches have been made to better understand this important interplay. For example, metabolites derived from GM shown promote atherosclerosis, underlying cause cardiovascular (CVD), increase CVD risk factors. Popular interest role intestine a variety states now resulted significant proportion individuals without coeliac switching gluten-free diets. effect diets on...
Peptides are notoriously known to display very short in vivo half-lives often measured minutes which many cases greatly reduces or eliminates sufficient efficacy. To obtain long allowing for up once-weekly dosing regimen, fatty acid acylation (lipidation) have been used non-covalently associate the peptide serum albumin thus serving as a circulating depot. This approach is generally considered scientific and patent community standard protract almost any given peptide. However, it not trivial...
Abstract Background Obesity affects nearly a billion people globally and is associated with various health consequences. Current anti-obesity medications primarily target appetite, but drug candidates that modulate energy expenditure (EE) substrate utilization based on respiratory exchange ratio (RER) are also essential to continuously improve the treatment modalities for living obesity. Selecting appropriate animal models methods crucial improving translational value in preclinical...
Abstract Most metabolic studies on mice are performed at room temperature, although under these conditions mice, unlike humans, spend considerable energy to maintain core temperature. Here, we characterize the impact of housing temperature expenditure (EE), homeostasis and plasma concentrations appetite- glucoregulatory hormones in normal-weight diet-induced obese (DIO) C57BL/6J fed chow or 45% high-fat-diet, respectively. Mice were housed for 33 days 22, 25, 27.5, 30 °C an...
The use of animal models in diabetes research requires reliable tests for evaluation insulin sensitivity and beta-cell function. Minipigs are being increasingly used metabolic research, the aim this study was to compare different indexes function Göttingen minipigs. Hyperinsulinemic, isoglycemic clamp, intravenous (IVGTT) oral glucose tolerance (OGTT), a modified test were performed minipigs fed either low- or high-energy diet. Furthermore, reproducibility IVGTT-derived parameters assessed....
Abstract Objective: Gender and sex hormones influence the development of obesity metabolic syndrome in humans Göttingen minipigs. The aim this study was to investigate possible gender differences response a high energy diet young minipigs as model childhood/adolescent obesity. Design Methods: Nine‐week‐old male female were fed restrictedly on either low (LED) or (HED) for 4 months ( n = 5‐7). Parameters interest fat percentage, visceral mass, plasma lipids glucose tolerance, insulin...
Non-alcoholic fatty liver disease (NAFLD) is the most common in humans, and ranges from steatosis to non-alcoholic steatohepatitis (NASH), latter with risk of progression cirrhosis. The Göttingen Minipig has been used studies obesity diabetes, but changes have not described. aim this study was characterize hepatic Minipigs or without fed a diet high fat, fructose, cholesterol see if alterations resemble features human NAFLD/NASH.Fifty-four male castrated minipigs (age 6 7 months) were...
Cholecystokinin (CCK) is a regulator of appetite and energy intake in man. The aim this study was to determine the effect NN9056, long-acting CCK-1 receptor-selective CCK analogue, on food body weight (BW) obese Göttingen Minipigs. Tolerability NN9056 acute effects intake, pancreas histology, amylase lipase levels were assessed lean domestic pigs doses up 100 nmol/kg (n = 3–4). Subsequently, Minipigs treated subcutaneously (s.c.) once daily for 13 weeks with vehicle, low dose (regulated from...
From a pharmacological perspective, readily-available, well-characterized animal models of cardiovascular disease, including relevant in vivo markers atherosclerosis are important for evaluation novel drug candidates. Furthermore, considering the impact diabetes mellitus on human patients, inclusion this disease aspect characterization such model, is highly relevant. The objective study was to evaluate effect mild streptozotocin-induced ex- and end-points diet-induced atherosclerotic minipig...
In the apolipoprotein E-deficient mouse, gut microbiota has an impact on development of atherosclerosis, but whether such correlations are also present in rats requires investigation. Therefore, we studied female SD-Apoe tm1sage (Apoe-/-) fed either a Western diet or low-fat control with without gluten, which is known to promote changes, until 20 weeks age. We hypothesized that manifestation atherosclerosis would be more severe Apoe-/- high-fat diet, as compared and accelerated by gluten....
Abstract Background Gut microbiota dysbiosis is associated with the development of non-alcoholic steatohepatitis (NASH) through modulation gut barrier, inflammation, lipid metabolism, bile acid signaling and short-chain fatty production. The aim this study was to describe impact a choline-deficient amino defined high fat diet (CDAHFD) on in male Göttingen Minipig model selected pathways implicated NASH. Results Eight weeks CDAHFD resulted significantly altered colon mainly driven by...
Evaluation of weight loss drugs is usually performed in diet-induced obese mice housed at ∼22°C. This a cold stress that increases energy expenditure by ∼35% compared to thermoneutrality (∼30°C), which may overestimate drug-induced loss. We investigated five anti-obesity mechanisms have been clinical development, comparing 22°C vs. 30°C. Glucagon-like peptide-1 (GLP-1), human fibroblast growth factor 21 (hFGF21), and melanocortin-4 receptor (MC4R) agonist induced similar losses. Peptide YY...
Summary Obesity has reached epidemic proportions globally and become the cause of several major health risks worldwide. Presently, more than 100 loci have been related to obesity metabolic traits in humans by genome‐wide association studies. The complex genetic architecture behind triggered a need for development better animal models rodents. pig emerged as very promising biomedical model study human traits. In this study, we characterized expression patterns six obesity‐related genes,...
Owing to the human-like physiology, a minipig model of nonalcoholic steatohepatitis (NASH) could be valuable. Pigs, however, rarely develop substantial hepatic steatosis, even when fed diets with high fat, fructose, and cholesterol (FFC) content. The potential choline-deficient, amino acid-defined high-fat (CDAHFD) was therefore evaluated in Göttingen Minipigs.Castrated male Minipigs were either chow (n = 5) or one three NASH diets: FFC 5), CDAHFD sucrose (CDAHFD-S; n 4), fructose (CDAHFD-F;...
Abstract Obesity and diabetes in humans are associated with hypertrophic remodeling increased media:lumen ratio of small resistance arteries, which is an independent predictor cardiovascular events. In order to minimize increases ratio, should be accompanied by outward remodeling. We aimed investigate the mechanisms structural pial arteries (PAs) terminal mesenteric (TMAs) from obese Göttingen Minipigs or without diabetes. received either control diet (lean (LC)), high fat/high fructose/high...
Abstract Background Model animals are valuable resources for dissecting basic aspects of the regulation obesity and metabolism. The translatability results relies on understanding comparative molecular pathophysiology. Several studies have shown that despite presence overt dyslipidemia in pig key human pathological hepatic findings such as hepatocellular ballooning abundant steatosis lacking model. Objectives aim this study was to elucidate why these histopathological characteristics did not...