A5020942299- Microbial infections and disease research
- Veterinary medicine and infectious diseases
- Viral gastroenteritis research and epidemiology
- Infant Nutrition and Health
- Aquaculture disease management and microbiota
- Bacteriophages and microbial interactions
- Gut microbiota and health
- Animal Behavior and Welfare Studies
- Clinical Nutrition and Gastroenterology
- Clostridium difficile and Clostridium perfringens research
- Probiotics and Fermented Foods
- Humic Substances and Bio-Organic Studies
- Insect and Pesticide Research
- Animal Disease Management and Epidemiology
- Animal health and immunology
- Mycobacterium research and diagnosis
- Streptococcal Infections and Treatments
- Animal Virus Infections Studies
- Parasitic Infections and Diagnostics
- Neonatal Respiratory Health Research
- Yersinia bacterium, plague, ectoparasites research
- Vector-borne infectious diseases
- Infective Endocarditis Diagnosis and Management
- Dermatology and Skin Diseases
- Milk Quality and Mastitis in Dairy Cows
Aarhus University Hospital
2024
Norwegian Veterinary Institute
2007-2024
Technical University of Denmark
2009-2020
University of Copenhagen
2019-2020
Sygehus Sønderjylland
2016-2018
Rigshospitalet
2013
Ecologie Microbienne Lyon
2002-2012
Aarhus University
2008
Danish Ministry of Food, Agriculture and Fisheries
2004-2007
National Veterinary Institute
2006
ABSTRACT The phylogenetic diversity of the intestinal bacterial community in pigs was studied by comparative 16S ribosomal DNA (rDNA) sequence analysis. Samples were collected from a total 24 representing variety diets, ages, and herd health status. A library comprising 4,270 cloned rDNA sequences obtained directly PCR 52 samples either ileum, cecum, or colon constructed. In total, 375 phylotypes identified using 97% similarity criterion. Three hundred nine (83%) had <97% to any database...
Preterm neonates have an immature gut and metabolism may benefit from total parenteral nutrition (TPN) before enteral food is introduced. Conversely, delayed feeding inhibit maturation sensitize to necrotizing enterocolitis (NEC). Intestinal mass NEC lesions were first recorded in preterm pigs fed enterally (porcine colostrum, bovine or formula for 20–40 h), with without a preceding 2- 3-day TPN period ( n = 435). Mucosal increased during further after reach intestinal similar that (+60–80%...
Background Recent evidence suggests that the gut microbiota is an important contributing factor to obesity and related metabolic disorders, known as syndrome. The aim of this study was characterise intestinal in two pig models namely Göttingen minipigs Ossabaw minipigs. Methods Findings cecal, ileal colonic from lean obese Osabaw were investigated by Illumina-based sequencing high throughput qPCR, targeting 16S rRNA gene different phylogenetic groups bacteria. weight gain through significant...
ABSTRACT The etiopathogenesis of the skin disease digital dermatitis (DD), an important cause lameness in cattle, remains uncertain. Microscopically, appears to be polymicrobial, with spirochetes as predominant bacteria. objective this study was identify main part bacteria involved DD lesions cattle by using culture-independent molecular methods. Ten different phylotypes Treponema were identified either 16S rRNA gene sequencing from or fluorescence situ hybridization (FISH) analysis...
Necrotizing enterocolitis (NEC) is the most common gastrointestinal emergency in newborn neonates. Bacteria are believed to be important pathogenesis of NEC but bacterial characterization has only been done on human faecal samples and experimental animal studies. The aim this study was investigate microbial composition relative number bacteria inflamed intestinal tissue surgically removed from neonates diagnosed with (n=24). populations specimens were characterized by laser capture...
Preterm birth and formula feeding predispose to necrotizing enterocolitis (NEC) in infants. As mother's milk is often absent following preterm delivery, infant (IF) human donor (HM) are frequently used as alternatives. We have previously shown that porcine bovine colostrum (BC) provide similar NEC protection piglets relative IF. hypothesized HM exerts effects this effect partly species-independent. (n = 40) received 2 days of total parenteral nutrition, followed by a rapid transition full...
In recent years, new neonatal porcine diarrhoea (NNPD) of unknown aetiology has emerged in Denmark. NNPD affects piglets during the first week life and results impaired welfare, decreased weight gain, worst-case scenario death. Commonly used preventative interventions such as vaccination or treatment with antibiotics, have a limited effect on NNPD. Previous studies investigated clinical manifestations, histopathology, to some extent, microbiological findings; however, these were either...
Although treponemes are consistently identified in tissue from bovine digital dermatitis (DD) lesions, the definitive etiology of this debilitating polymicrobial disease is still unresolved. To study microbiomes 27 DD-infected and 10 healthy interdigital skin samples, we used a combination different molecular methods. Deep sequencing 16S rRNA gene variable regions V1-V2 showed that Treponema, Mycoplasma, Fusobacterium Porphyromonas were genera best differentiating DD samples controls....
ABSTRACT The bacteria associated with the infectious claw disease bovine digital dermatitis (DD) are spirochetes of genus Treponema ; however, their environmental reservoir remains unknown. To our knowledge, current study is first report discovery and phylogenetic characterization rRNA gene sequences from DD-associated treponemes in dairy herd environment. Although spread DD appears to be facilitated by wet floors covered slurry, no have been isolated this environment previously....
To investigate which specific bacterial species that were stimulated or inhibited in the proximal colon of pigs when a fructan-rich diet was compared with contained resistant carbohydrates. The study focussed especially on Bifidobacterial by using noncultureable approach.
The acute phase protein response is a well-described generalized early host to tissue injury, inflammation and infection, observed as pronounced changes in the concentrations of number circulating serum proteins.The biological function this its interplay with other parts innate defence reactions remain somewhat elusive.In order gain new insight into context bacterial infection we studied gene expression peripheral lymphoid tissues compared hepatic changes, 14-18 h after lung pigs.The was...
Preterm neonates are susceptible to gastrointestinal disorders such as necrotizing enterocolitis (NEC). Maternal milk and colostrum protects against NEC via growth promoting, immunomodulatory, antimicrobial factors. The fetal enteral diet amniotic fluid (AF), contains similar components, we hypothesized that postnatal AF administration reduces inflammatory responses in preterm neonates. pigs (92% gestation) were delivered by caesarean section fed parental nutrition (2 days) followed porcine...
Abstract Wolbachia are renowned as reproductive parasites, but their phenotypic effects in eusocial insects not well understood. We used a combination of qrt‐PCR, fluorescence situ hybridization and laser scanning confocal microscopy to evaluate the dynamics infections leaf‐cutting ant Acromyrmex octospinosus across developmental stages sterile workers. confirm that workers infected with one or two widespread wsp genotypes , show colony prevalence is always 100% characterize rare recombinant...
ABSTRACT Modern pyrosequencing technology allows for a more comprehensive approach than traditional Sanger sequencing elucidating the etiology of bovine digital dermatitis. We sought to describe composition and diversity treponemes in dermatitis lesions by using deep V3 V4 hypervariable regions 16S rRNA gene coupled with species-level taxonomic identification. Treponema -specific PCRs were performed on biopsy specimens originating from 10 different Catalan dairy herds ( n = 36) dermatitis,...
A rapid advance in enteral feeding is associated with necrotizing enterocolitis (NEC) preterm infants. Therefore, minimal nutrition (MEN) combined parenteral (PN) common clinical practice, but the effects on NEC and intestinal function remain poorly characterized. It was hypothesized that a commonly used MEN volume (16-24 mL/kg/d) prevents improves structure, function, microbiology pigs.After birth pigs were stratified into 4 intervention groups received following treatments: (1) PN followed...
Abstract Background Obesity induced by a high-caloric diet has previously been associated with changes in the gut microbiota mice and humans. In this study, pigs were cloned to minimize genetic biological variation among animals aim of developing controlled metabolomic model suitable for diet-intervention study. Cloning may be an attractive way reduce influences when investigating effect obesity on different physiological sites. The study was assess compare composition vs. non-cloned during...