A5000958881- Gut microbiota and health
- Diet and metabolism studies
- MicroRNA in disease regulation
- Clostridium difficile and Clostridium perfringens research
- Dietary Effects on Health
- Adipose Tissue and Metabolism
- Bacteriophages and microbial interactions
- Cancer-related molecular mechanisms research
- Epigenetics and DNA Methylation
- Circular RNAs in diseases
- Parasitic Infections and Diagnostics
- Adipokines, Inflammation, and Metabolic Diseases
- Viral gastroenteritis research and epidemiology
- Parasites and Host Interactions
- Diabetes and associated disorders
- Immune cells in cancer
- Fibroblast Growth Factor Research
- RNA Research and Splicing
- Identification and Quantification in Food
- Cancer-related gene regulation
- Asthma and respiratory diseases
- Helminth infection and control
- IL-33, ST2, and ILC Pathways
- Sperm and Testicular Function
- Celiac Disease Research and Management
University of Copenhagen
2014-2024
Freie Universität Berlin
1976-1977
Development of obesity and type 2 diabetes (T2D) are associated with gut microbiota (GM) changes. The viral community is predominated by bacteriophages (phages), which viruses that attack bacteria in a host-specific manner. antagonistic behaviour phages has the potential to alter GM. As proof-of-concept, we demonstrate efficacy faecal virome transplantation (FVT) from lean donors for shifting phenotype obese mice into closer resemblance mice.The FVT consisted viromes distinct profiles...
The prevalence of non-alcoholic fatty liver disease (NAFLD) has increased dramatically worldwide and, subsequently, also the risk developing steatohepatitis (NASH), hepatic fibrosis, cirrhosis and cancer. Today, weight loss is only available treatment, but administration fibroblast growth factor 21 (FGF21) analogues have, in addition to loss, shown improvements on metabolic health mechanisms behind are not entirely clear. aim this study was investigate profile response FGF21 treatment....
Probiotics are intended to improve gastrointestinal health when consumed. However, the probiotics marketed today only colonize densely populated gut a limited extent. Bacteriophages comprise majority of viruses in human virome and there strong indications that they play important roles shaping microbiome. Here, we investigate use fecal transplantation (FVT, sterile filtrated feces) as mean alter microbiome composition lead way for persistent colonization two types probiotics:...
Obesity is a complex condition that increases the risk of life threatening diseases such as cardiovascular disease and diabetes. Studying gene regulation obesity important for understanding molecular mechanisms behind derived may lead to better intervention treatment plans. MicroRNAs (miRNAs) are short non-coding RNAs regulating target mRNA by binding their 3'UTR. They involved in numerous biological processes diseases, including obesity. In this study we use mixed breed pig model designed...
Obesity is associated with immunological perturbations that contribute to insulin resistance. Epigenetic mechanisms can control immune functions and have been linked metabolic complications, although their contribution resistance still remains unclear. In this study, we investigated the link between dysfunction alterations epigenetic signature in leukocytes a porcine model of obesity. Global DNA methylation circulating leukocytes, adipose tissue leukocyte trafficking, macrophage polarisation...
Obesity and its comorbidities are an increasing challenge for both affected individuals health care systems, worldwide. In obese individuals, perturbation of expression protein-coding genes microRNAs (miRNA) seen in obesity-relevant tissues (i.e. adipose tissue, liver skeletal muscle). miRNAs small non-coding RNA molecules which have important regulatory roles a wide range biological processes, including obesity. Rodents widely used animal models human diseases However, not all research is...
Objective . The importance of early microbial dysbiosis in later development obesity and metabolic disorders has been a subject debate. Here we tested cause effect mice. Methods Germ‐free male Swiss Webster mice were colonized specific‐pathogen‐free (SPF) facility at 1 week (1W) 3 weeks (3W) age. They challenged with high‐fat diet their responses compared SPF Gut microbiota was analyzed by 16S rRNA gene sequencing. Moreover, RNA sequencing the liver performed on additional 3W regular chow...
Reprogramming of adipocyte function in obesity is implicated metabolic disorders like type 2 diabetes. Here, we used the pig, an animal model sharing many physiological and pathophysiological similarities with humans, to perform in-depth epigenomic transcriptomic characterization pure fractions. Using a combined DNA methylation capture sequencing Reduced Representation bisulfite (RRBS) strategy 11 lean 12 obese pigs, identified 3529 differentially methylated regions (DMRs) located at close...
Abstract Group sizes in an animal study are calculated from estimates on variation, effect, power and significance level. Much of the variation glucose related parameters diet-induced obese (DIO) mouse model is due to inter-individual gut microbiota composition. In addition, standard tandem repeats (STRs) non-coding DNA shows that inbred mice not always homogenic. C57BL/6NTac (B6NTac) Taconic C57BL/6NRj (B6NRj) Janvier Labs were fed a high calorie diet treated with liraglutide. The fecal was...
MicroRNAs are key post-transcriptional regulators of gene expression that involved in several biological processes including those mediate disease pathophysiology. Hence, quantifying microRNA levels can provide important and novel insights into biology. In recent years, the pig has emerged as an excellent large animal model for studying human diseases conditions (e.g. obesity) due to similarities organ size, gastro-intestinal tract, metabolism, immune response, genetics availability relevant...
Abstract Background The influence of diet on immune function and resistance to enteric infection disease is becoming ever more established. Highly processed, refined diets can lead inflammation gut microbiome dysbiosis, whilst health-promoting dietary components such as phytonutrients fermentable fibres are thought promote a healthy balanced mucosal immunity. Chicory ( Cichorium intybus ) leafy green vegetable rich in bioactive compounds that may health. Results Unexpectedly, we here show...
The binding of antibodies against LDH-X by preimplantation mouse embryos was studied to detect from spermatozoa in after fertilization. Incubation with rabbit anti-mouse-LDH-X-IgG and then peroxidase-labelled goat anti-rabbit IgG revealed a strong peroxidase staining the zona pellucida normal fertilized unfertilized 1-cell ova. However, reaction significantly weaker both ova females induced superovulate superovulated blastocysts. Pure antibody obtained affinity chromatography anti-mouse...
ABSTRACT Objective Development of obesity and type-2-diabetes (T2D) are associated with gut microbiota (GM) changes. The viral community is predominated by bacteriophages (phages), which viruses that attack bacteria in a host-specific manner. As proof-of-concept we demonstrate the efficacy faecal virome transplantation (FVT) from lean donors for shifting phenotype obese mice into closer resemblance mice. Design FVT consisted viromes extracted caecal content fed low-fat (LF) diet fourteen...
Abstract Several mammalian species are vaccinated in early life, but little is known about the effect of diet on vaccine response. Oligosaccharides increasingly proposed as dietary supplement for young individuals due to their anti-inflammatory potential elicited through modulation gut microbiota (GM). Also, diet, e.g. size fat fraction, modulate GM. We tested if an oligosaccharide (Immulix) and/or increased content affected antibody titers a tetanus 48 BALB/cJTac mice GM modulation. Female...
Weight gain variation is a great challenge in diet-induced obesity studies since low-gainer animals are of limited experimental value. The inbred C57BL/6 (B6) mice frequently used models due to their genetic homogeneity and susceptibility (DIO). aim this study investigate if the gut microbiota (GM) influences fraction low weight gainers DIO studies. A total 100 male B6 (donor population) were fed high-fat diet for 14 weeks divided into groups high gainer (HG) (LG) based on gain....
Delivery by cesarean section (CS) is associated with an altered gut microbiota (GM) colonization and a higher risk of later chronic inflammatory diseases. Studies investigating the association between CS atopic dermatitis (AD) are contradictive often biased confounding factors. The aim this study was therefore to provide experimental evidence for AD in mouse model clarify role GM changes CS. It hypothesized that CS-delivered mice, human CS-GM transplanted mice develop severe due early...
Abstract Anorexia nervosa (AN) is a complex and serious mental disorder, which may affect individuals of all ages sex, but primarily affecting young women. The disease characterized by disturbed body image, restrictive eating behavior, lack acknowledgment low weight. underlying causes AN remain largely unknown, current treatment options are limited to psychotherapy nutritional support. This paper investigates the impact Fecal Microbiota Transplants (FMT) from patients on food intake, weight,...
Abstract Probiotics have been suggested as nutritional supplements to improve gastrointestinal health. However, the probiotics marketed today only colonize densely populated gut a limited extent. Bacteriophages comprise majority of viruses in human virome and there are strong indications that they play important roles shaping microbiome (GM). Here we investigate use fecal transplantation (FVT) mean alter GM composition lead way for persistent colonization two types probiotics:...