ADVERTISEMENT RETURN TO ISSUEPREVFEATURESNEXTWatching Silica Nanoparticles Glow in the Biological WorldLin Wang, Kemin Swadeshmukul Santra, Xiaojun Zhao, Lisa R. Hilliard, Joshua E. Smith, Yanrong Wu, and Weihong TanCite this: Anal. Chem. 2006, 78, 3, 646–654Publication Date (Web):February 1, 2006Publication History Published online1 February 2006Published inissue 1 2006https://doi.org/10.1021/ac0693619RIGHTS & PERMISSIONSArticle Views5629Altmetric-Citations305LEARN ABOUT THESE...

In this study, a series of di-O-caffeoylquinic acids (di-COQs) were systematically investigated for their antioxidant and cytoprotective effects towards •OH-damaged bone marrow-derived mesenchymal stem cells (bmMSCs). Five di-COQs measured using set assays. The results show that adjacent 4,5-Di-O-caffeoylquinic acid (4,5-COQ) 3,4-di-O-caffeoylquinic (3,4-COQ) always gave lower IC50 values than did non-adjacent di-COQs. the Fe2+-chelating assay, 4,5-COQ 3,4-COQ presented greater UV-Vis...

Advancements in electrical components have intensified the challenges for copper alloy wear resistance and high-temperature performance applications. The surface coating preparation of Cu alloys is crucial enhancing their lifespan promoting sustainable resource development. This study explored microstructure properties Cu-Cr-X coatings (X = Mo/W, Al2O3/TiO2) on substrates via laser-cladding to improve hardness, vital component reliability switching capacity. process involved adjusting power...

Cancer-cell phenotype is not only the result of malignant progression, but also dependent on microenvironment surrounding them, including influences from extracellular matrix and its structural properties. We have investigated influence nanofiber self-assembling peptide, RADA16, in comparison with collagen I Matrigel human breast-cancer cell, MDA-MB-231, 3D cultures, morphology, survival, proliferation rate, migration potential effect these matrices malignancy cancer cells vivo. Our data...

Functionalized self-assembling peptide improves INS-1 β-cell function and proliferation via the integrin/FAK/ERK/cyclin pathway Jingping Liu,1 Shuyun Younan Chen,1 Xiaojun Zhao,2 Yanrong Lu,1 Jingqiu Cheng1 1Key Laboratory of Transplant Engineering Immunology, Regenerative Medicine Research Center, 2Laboratory Nanomedicine, West China Hospital, Sichuan University, Chengdu, People’s Republic Abstract: Islet transplantation is considered to be a curative treatment for type 1 diabetes mellitus....

As a phenolic alkaloid occurring in Cruciferous plants, sinapine was observed to protect mesenchymal stem cells (MSCs) against ·OH-induced damage this study. It also found prevent DNA from damage, scavenge various free radicals (·OH, ·O2−, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid diammonium salt) (ABTS)+·, and 1,1-diphenyl-2-picrylhydrazyl radical (DPPH·)), reduce Cu2+ Cu+. To further explore the mechanism, end-product of reaction with DPPH· determined using HPLC-electrospray...

A newly designed self-assembling peptide, P4 (Ac-NH-LDLKLELKLDLKLELK-CONH2), capable of stabilizing hydrophobic compounds in aqueous solution has been discovered. The ionic self-complementary peptide 16 amino acids, ∼5 nm size, with an alternating polar and non-polar pattern. Circular dichroism analysis demonstrated that forms stable β-sheet structures, self-assembles into nanofibers, which was by atomic force microscopy. These nanofibers can form a scaffold hydrogel consisting >99% water....

To clarify the role of B-ring in antioxidant flavonols, we performed a comparative study between galangin with null and 3,5,7-trihydroxychromone without using five spectrophotometric assays, namely, •O₂--scavenging, 1,1-diphenyl-2-picrylhydrazyl radical (DPPH•)-scavenging, 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide radical-scavenging, 2,2'-azino-bis(3-ethylbenzo-thiazoline-6-sulfonic acid) Fe3+-reducing activity. The DPPH•-scavenging reaction products these assays were further...

This study used the 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl 3-oxide radical (PTIO•) trapping model to antioxidant activities of 16 natural xanthones in aqueous solution, including garcinone C, γ-mangostin, subelliptenone G, mangiferin, 1,6,7-trihydroxy-xanthone, 1,2,5-trihydroxyxanthone, 1,5,6-trihydroxyxanthone, norathyriol, 1,3,5,6-tetrahydroxy-xanthone, isojacareubin, 1,3,5,8-tetrahydroxyxanthone, isomangiferin, 2-hydroxyxanthone, 7-O-methylmangiferin, neomangiferin, and lancerin....

Abstract Jieyu Anshen Granule is a traditional Chinese medicine prescription used for depression and primarily comprises five herbal medicines: Zhizi, Chaihu, Zhigancao, Danggui, Yuanzhi. This study established medicine‐specific library using emerging ultra‐high‐performance liquid chromatography‐quadrupole‐orbitrap‐tandem mass spectrometry analysis. Through comparison, the has fulfilled isomers distinction. As result, 47 compounds were simultaneously putatively identified from Granule,...

The self-assembling peptide, RAD16-II (Ac-RARADADARARADADA-NH2), has been shown to have many great applications in nanotechnology and tissue engineering. But few studies carried out study the application of peptides as a drug carrier. We used hydrophobic pyrene model liposome vesicles mimic bilayer membranes ability encapsulation transfer lipid vesicles. It was found that this designed peptide able stabilize aqueous solution deliver it into lipophilic environment. Analysis fluorescence...

HspB8/Hsp22 is a functionally distinct small heat shock proteins (sHsp) and preferentially expressed in brain, heart, skeletal, smooth muscle. HspB8 also associated with neuromuscular function protein quality control by proteasomes cardiac hypertrophy. However, the molecular properties vitro oligomerization remain uncertain. In this investigation, rat gene was E.coli cells, mature efficiently prepared. The chaperone-like activity of quantitatively analyzed model substrates. Size exclusion...

D-Alanine:D-Alanine ligase (DDl) catalyzes the formation of dipeptide and is an essential enzyme in bacterial cell wall biosynthesis. This does not have a human ortholog, making it attractive target for developing new antibiotic drugs. We determined crystal structure at 2.23 Å resolution DDl from Streptococcus mutans (SmDDl), principal aetiological agent dental caries. reveals that SmDDl dimer has disordered ω-loop region. Keywords: X-ray structure, amino acids peptides, DDl,

Spider silk is intriguing because of its unique structure and high-performance mechanical properties. However, few studies have presented data characterizing the conserved motifs found in protein as an oligopeptide. In this report a designed peptide that mimics repetitive GGX motif spidroin was investigated. The undergoes 'bottom-up' self-assembly which simply manipulated by metal ions ionic self-complementary peptide, RADA16-I. water, observed to form discrete stiff nanorods (PNrs) via...

The cardiovascular heat shock protein (cvHsp/HspB7) exhibited cardiac-specific expression and is a possible candidate of dilated cardiomyopathy in heart failure. molecular characteristics biochemical properties cvHsp are only partially understood. This study was aimed to identify the biological high-order structure cvHsp. prepared by refined purification at large amount. pooled fractions were existed as two types oligomers solution chaperone-like activity. circular dichroism analyzed...

The thrC gene of Streptococcus mutans encodes threonine synthase, which is a potential target for drug design. To study the structure and function enzyme, was amplified from genomic DNA cloned into expression vector pET28α. protein expressed in Escherichia coli soluble form purified to homogeneity. Crystals suitable X-ray diffraction were obtained by hanging-drop vapor diffusion method. crystal diffracted 2.5 Å belonged space group P31 or P32, with unit cell parameters a=b=60.39 Å, c=118.62...

A typically designed 'Peptide Lego' has two distinct surfaces: a hydrophilic side that contains the complete charge distribution and hydrophobic side. In this article, we describe fabrication of unique lego-type peptide with AEAEYAKAK sequence. The novel double amphiphilic surfaces is different from typical peptides due to special arrangement residues. results CD, FT-IR, AFM DLS demonstrate random coil characteristic was able form stable nanostructures were mediated by non-covalent...

The smu.134 gene encodes a putative transcriptional regulator of 217 residues in Streptococcus mutans, major pathogen for human dental caries. was cloned into expression vector pET28alpha and expressed soluble form E. coli strain BL21 (DE3) with His tag at its N-terminus. recombinant protein SMU.134 purified to homogeneity two step procedure Ni(2+ ) chelating size exclusion chromatography. Crystals suitable X-ray diffraction were obtained by hanging-drop vapor diffusion method diffracted 2.6...

In recent years, the study of self-assembly peptide used in drug delivery system has been attracted great interest from scientists. category are peptides structure either with one hydrophobic surface and another hydrophilic or a head tail. Here, we focus on novel designed EYK double amphiphilic surfaces, investigating capability as carrier for compound model pyrene. The fluorescence data presented dynamic process transfer, showing that pyrene was crystalline form solution, molecularly...