Background: Protocatechuic acid (PCA) is a natural phenolic widely distributed in plants and considered as an active component of some traditional Chinese herbal medicines such Cibotium barometz (L.) J.Sm, Stenoloma chusanum Ching, Ilex chinensis Sims. PCA was reported to possess various pharmacological effects which may be closely correlated with its antioxidant activities. However, the has not been investigated systematically yet. Methods: In study, activities protocatechuic were measured...

Gallic acid is an active phenolic widely distributed in plants, and there compelling evidence to prove its anti-inflammatory effects. NLRP3 inflammasome dysregulation closely linked many inflammatory diseases. However, how gallic affects the remains unclear. Therefore, present study, we investigated mechanisms underlying effects of on pyroptosis, as well effect gouty arthritis mice. The results showed that inhibited lactate dehydrogenase (LDH) release pyroptosis lipopolysaccharide...

As a typical Chinese herbal medicine, rhizoma Cimicifugae (RC, in Chinese) possesses various pharmacological effects involved antioxidant activity. However, its activity has not been reported so far. The aim of the present study was to systematically evaluate ability RC vitro, then discuss mechanism.Firstly, five extracts (i.e. petroleum ether extract PERC, ethyl acetate EARC, absolute ethanol AERC, 95% 95ERC, and water WRC) were prepared determined by methods, including...

The role of the 6″-OH (ω-OH) group in antioxidant activity flavonoid glycosides has been largely overlooked. Herein, we selected quercitrin (quercetin-3-O-rhamnoside) and isoquercitrin (quercetin-3-O-glucoside) as model compounds to investigate several pathways, including Fe2+-binding, hydrogen-donating (H-donating), electron-transfer (ET). results revealed that both exhibited dose-dependent activities. However, showed higher levels than ET-based ferric ion reducing power,...

Polysaccharide (DOPA) from the stem of D. officinale, as well two fractions (DOPA-1 and DOPA-2) it, were isolated purified by DEAE cellulose-52 Sephacryl S-300 chromatography, their structural characteristics bioactivities investigated. The average molecular weights DOPA-1 DOPA-2 394 kDa 362 kDa, respectively. They mainly composed d-mannose, d-glucose, had a backbone consisting 1,4-linked β-d-Manp β-d-Glcp with O-acetyl groups. Bioactivity studies indicated that both DOPA its could activate...

The study determined the comparative antioxidant capacities of five similar dihydrochalcones: phloretin, phloridzin, trilobatin, neohesperidin dihydrochalcone, and naringin dihydrochalcone. In ferric-reducing power (FRAP) assay, activities pairs dihydrochalcones had following relationship: phloretin > trilobatin dihydrochalcone Similar relative levels were also obtained from 1,1-diphenyl-2-picryl-hydrazl radical (DPPH•)-scavenging, 2,2′-azino-bis(3-ethylbenzo-thiazoline-6-sulfonic acid)...

The modification of methyltransferase-like (METTL) enzymes plays important roles in various cellular responses by regulating microRNA expression. However, how m6A is involved stress granule (SG) formation the early stage acute ischemic stroke affecting biogenesis processing microRNAs remains unclear. Here, we established a middle cerebral artery occlusion (MCAO) model rats and an oxygen-glucose deprivation/reperfusion (OGD/R) primary cortical neurons PC12 cells to explore potential mechanism...

Ferroptosis is a type of cell death induced by the iron-dependent accumulation lipid hydroperoxides and reactive oxygen species (ROS) in cells. Inhibiting ferroptosis important for improving survival transplanted bone marrow-derived mesenchymal stem cells (BMSCs). Although it known that NOP2/Sun RNA methyltransferase 5 (NSUN5) post-transcriptionally regulates BMSCs through methylation, precise mechanisms underlying these effects have not been reported. In this study, we demonstrate NSUN5...

Corilagin, a gallotannin, shows excellent antioxidant and anti-inflammatory effects. The NLRP3 inflammasome dysfunction has been implicated in variety of inflammation diseases. However, it remains unclear how corilagin regulates the to relieve gouty arthritis. In this study, bone marrow-derived macrophages (BMDMs) were pretreated with lipopolysaccharide (LPS) then incubated agonists, such as adenine nucleoside triphosphate (ATP), nigericin, monosodium urate (MSU) crystals. MSU crystals...

To determine the roles of glial fibrillary acidic protein (GFAP) and vimentin in Müller cell reactivity.Retinal detachments were created mice deficient for GFAP (GFAP(-/-)vim(-/-)) age-matched wild-type (wt) mice. The reactivity retina was studied by immunofluorescence electron microscopy.Müller morphology different glutamine synthetase immunoreactivity reduced undisturbed GFAP(-/-)vim(-/-) retinas. After retinal detachment, cells formed subretinal scars wt In contrast, such not observed...

Rhizoma Atractylodes macrocephala (AM) has been used in Traditional Chinese Medicine (TCM) for about 2,000 years. In the study, we firstly determined antioxidant levels of five AM extracts by •OH-scavenging, •O2−-scavenging, Fe2+-chelating, Cu2+-chelating, DPPH·-scavenging, and ABTS+·-scavenging assays. After measurement chemical contents extracts, quantitatively analyzed correlations between contents. It was observed that total phenolics flavonoids had significant positive with (R = 0.685...

// Jun Wang 1 , Zeng-jie Lei Yan Guo Tao Zhong-yi Qin Hua-liang Xiao 2 Li-lin Fan Dong-feng Chen Xiu-wu Bian 3 Jia Liu 4 Bin Department of Gastroenterology, Institute Surgery Research, Daping Hospital, Third Military Medical University, Chongqing 400042, China Pathology, Pathology and Southwest Cancer Center, Key Laboratory Tumor Immunopathology Ministry Education China, 400038, Translational Medicine, College Qingdao 266021, Correspondence to: Wang, e-mail: wb_tmmu@126.com Liu,...

Flos Chrysanthemi Indici (FCI) is a Chinese herbal medicine used in China for over 2000 years. In this study, its ethanol extract (EFCL) was found to protect against hydroxyl radical (OH) induced oxidative damages mesenchymal stem cells (MSCs) and DNA. To explore the mechanism, EFCL further determined by chemical antioxidant assays. Folin–Ciocalteu colorimetric assay suggested that content of total phenolics 93.85 ± 3.56 mg catechin/g HPLC analysis indicated 10.05 1.44 mg/g as chlorogenic...

In this study, the anti-ferroptosis effects of catecholic flavonol quercetin and its metabolite Diels-Alder anti-dimer (QDAD) were studied using an erastin-treated bone marrow-derived mesenchymal stem cell (bmMSCs) model. Quercetin exhibited higher levels than QDAD, as indicated by 4,4-difluoro-5-(4-phenyl-1,3-butadienyl)-4-bora-3a,4a-diaza-s-indacene-3-undecanoic acid (C11-BODIPY), 2′,7′-dichlorodihydrofluoroscein diacetate (H2DCFDA), lactate dehydrogenase (LDH) release, counting kit-8...

Abstract Objectives Wedelolactone exhibits regulatory effects on some inflammatory diseases. However, the anti‐inflammatory mechanism of wedelolactone has not been entirely unravelled. Therefore, present study focuses investigating NLRP3 inflammasome in macrophages and its influence MSU‐induced inflammation. Materials Methods BMDM, J774A.1 PMA‐differentiated THP‐1 were primed with LPS then stimulated ATP or nigericin MSU crystal presence absence wedelolactone. The cell lysates supernatants...

To date, it has repeatedly been demonstrated that infusing bone marrow-derived stem cells (BMSCs) into acellular nerve scaffolds can promote and support axon regeneration through a peripheral defect. However, harvesting BMSCs is an invasive painful process fraught with low cellular yield.In pursuit of alternative cell sources, we isolated from the inguinal subcutaneous adipose tissue adult Sprague-Dawley rats (adipose-derived cells, ADSCs). We used co-culture system allows mesenchymal (MSCs)...

In this study, a series of di-O-caffeoylquinic acids (di-COQs) were systematically investigated for their antioxidant and cytoprotective effects towards •OH-damaged bone marrow-derived mesenchymal stem cells (bmMSCs). Five di-COQs measured using set assays. The results show that adjacent 4,5-Di-O-caffeoylquinic acid (4,5-COQ) 3,4-di-O-caffeoylquinic (3,4-COQ) always gave lower IC50 values than did non-adjacent di-COQs. the Fe2+-chelating assay, 4,5-COQ 3,4-COQ presented greater UV-Vis...

Parkinson's disease (PD) is a neurodegenerative characterized by the selective loss of dopaminergic neurons in substantia nigra (SN). In previous study, authors demonstrated that ferritin heavy chain 1 (FTH1) inhibited ferroptosis model 6‑hydroxydopamine (6‑OHDA)‑induced PD. However, whether and how microRNAs (miRNAs/miRs) modulate FTH1 PD not yet well understood. present <em>in vivo</em> vitro</em> models induced 6‑OHDA were established. The results revealed levels marker protein,...

Effective carbon pricing policies have become an effective tool for many countries to encourage emission reduction. An accurate price prediction model is helpful the implementation of energy conservation and reduction decision-making governments investors. However, it difficult a single achieve high accuracy because complexity series. Many studies proved nonlinear characteristics trading prices, but there are very few on chaotic nature As consequence, this paper proposes innovative hybrid...