// Jing Ke 1, 2 , Yi-long Yao 3 Jian Zheng Ping Wang Yun-hui Liu Jun Ma Zhen Li Xiao-bai Zhi-qing Zhen-hua 4 Yi-xue Xue 1 Department of Neurobiology, College Basic Medicine, China Medical University, Shenyang 110122, Institute Pathology and Pathophysiology, Neurosurgery, Shengjing Hospital 110004, Physiology, Correspondence to: Xue, e-mail: xueyixue888@163.com Keywords: lncRNA, HOTAIR, miR-326, glioma, FGF1 Received: February 07, 2015      Accepted: June 17,...

// Jian Zheng 1 , Xiao-dong Li Ping Wang 2, 3 Xiao-bai Liu Yi-xue Xue Yi Hu Zhen Zhi-qing Zhen-hua 4 Yun-hui Department of Neurosurgery, Shengjing Hospital China Medical University, Shenyang 110004, 2 Neurobiology, College Basic Medicine, 110001, Institute Pathology and Pathophysiology, Physiology, Correspondence to: Liu, e-mail: liuyh_cmuns@163.com Keywords: long non-coding RNAs, CRNDE, microRNAs, miR-186, glioma stem cells Received: January 28, 2015      Accepted:...

Rationale: PIWI-interacting RNAs (piRNAs), a class of newly discovered small RNA molecules that function by binding to the Argonaute protein family (i.e., PIWIL subfamily), and long noncoding (lncRNA) are implicated in several cancers. However, detailed roles ncRNAs glioma remain unclear. Methods: The expression PIWIL3, piR-30188, OIP5-AS1, miR-367, CEBPA TRAF4 were measured tissues cells. role PIWIL3/OIP5-AS1/miR-367-3p/CEBPA feedback loop was evaluated cell animal models. association above...

// Heng Cai 1 , Yixue Xue 2,3 Ping Wang Zhenhua 4 Zhen Li Yi Hu Zhiqing Xiuli Shang 5 and Yunhui Liu Department of Neurosurgery, Shengjing Hospital China Medical University, Shenyang, People’s Republic 2 Neurobiology, College Basic Medicine, 3 Institute Pathology Pathophysiology, Physiology, Neurology, The First Affiliated Correspondence to: Liu, email: Keywords : glioma, TUG1, microRNA-144, blood-tumor barrier, HSF2 Received February 02, 2015 Accepted May 25, Published June Abstract...

Abstract Background Glioma is one of the most frequent intracranial malignant tumors. LncRNAs have been identified as new modulators in origination and progression glioma. Methods Quantitative real-time PCR were conducted to evaluate expression linc00152 miRNA-103a-3p glioma tissues cells. Western blot used determine FEZF1 CDC25A Stable knockdown or over-expression miR-103a-3p stem cells (GSCs) established explore function GSCs. Further, luciferase reports investigate correlation between...

Blood-tumor barrier (BTB) constitutes an efficient organization of tight junctions which significantly reduce permeability for chemotherapy drugs. Krüppel-like factor 4 (KLF4), a member the family, has been documented in endothelial cells and may serve as essential regulator function. However, our knowledge about expression function KLF4 BTB still remains unclear. In this study, we sought to investigate role regulation well potential molecular mechanisms. Quantitative RT-PCR, Western blot,...

In this study, the anti-ferroptosis effects of catecholic flavonol quercetin and its metabolite Diels-Alder anti-dimer (QDAD) were studied using an erastin-treated bone marrow-derived mesenchymal stem cell (bmMSCs) model. Quercetin exhibited higher levels than QDAD, as indicated by 4,4-difluoro-5-(4-phenyl-1,3-butadienyl)-4-bora-3a,4a-diaza-s-indacene-3-undecanoic acid (C11-BODIPY), 2′,7′-dichlorodihydrofluoroscein diacetate (H2DCFDA), lactate dehydrogenase (LDH) release, counting kit-8...

Abstract Dysregulation of long non‐coding RNAs (lncRNAs) confirm that it plays a crucial role in tumourigenesis and malignant progression glioma. The present study demonstrated LncRNA secretory carrier membrane protein 1 (SCAMP1) was up‐regulated functioned as an oncogene glioma cells. In addition, miR‐499a‐5p down‐regulated meanwhile exerted tumour‐suppressive function Subsequently, inhibition SCAMP1 significantly restrained the cell proliferation, migration invasion, well promoted...

Glioma is a brain cancer characterized by strong invasiveness with limited treatment options and poor prognosis. Recently, dysregulation of long non-coding RNAs (lncRNAs) has emerged as an important component in cellular processes tumorigenesis. In this study, we demonstrated that TATA-box binding protein associated factor 15 (TAF15) intergenic non-protein coding RNA 665 (LINC00665) were both downregulated glioma tissues cells. TAF15 overexpression enhanced the stability LINC00665,...

Glioblastomas are the most common and malignant intracranial tumors with a low survival rate. Dysregulation of long non-coding RNAs RNA-binding protein causes various diseases, including cancers. However, function LINC00680 TTN-AS1 in progression glioblastomas is still elusive. In this study, we detected that were upregulated glioblastoma cells. EIF4A3 could prolong half-life TTN-AS1. Knockdown EIF4A3, LINC00680, impaired proliferation, migration, invasion inhibited growth tumor vivo...

Gliomas, the most malignant form of brain tumors, contain a small subpopulation glioma stem cells (GSCs) that are implicated in therapeutic resistance and tumor recurrence. Topoisomerase I inhibitors, shikonin topotecan, play crucial role anti-cancer therapies. After isolated identified GSCs from successfully, U251, U87, GSCs-U251 GSCs-U87 were administrated with various concentrations or topotecan at different time points to seek for optimal administration concentration point. The cell...

Glioblastoma (GBM) is the most aggressive primary intracranial tumor of adults and confers a poor prognosis due to high vascularization. Hence anti-angiogenic therapy has become promising strategy for GBM treatment. In this study, transcription factor nuclear activated T-cells 5 (NFAT5) was significantly elevated in glioma samples cell lines, positively correlated with WHO grades. Knockdown NFAT5 inhibited cell-driven angiogenesis. Furthermore, long non-coding RNA SBF2 antisense 1 (SBF2-AS1)...

Great interest persists in useful therapeutic targets glioblastoma (GBM). Deregulation of microRNAs (miRNAs) expression has been associated with cancer formation through alterations gene targets. In this study, we reported the role miR-101 human stem cells (GSCs) and potential mechanisms.Quantitative real-time PCR showed that was decreased GSCs. Overexpression reduced proliferation, migration, invasion, promoted apoptosis One direct target miR-101, transcription factor Kruppel-like 6 (KLF6),...

Glioma is the most common intracranial neoplasm with vasculogenic mimicry formation as one form of blood supply. Many RNA-binding proteins and long non-coding RNAs are involved in tumorigenesis glioma. The expression ZRANB2, SNHG20 FOXK1 glioma were detected by real-time PCR or western blot. function ZRANB2/SNHG20/FOXK1 axis associated was analyzed. ZRANB2 up-regulated tissues cells. knockdown inhibits proliferation, migration, invasion binds to increases its stability. Knockdown reduces...

Long non-coding RNAs has been reported in tumorigenesis and play important roles regulating malignant behavior of cancers, including glioma. According to the TCGA database, we identified SNHG1, miRNA-154-5p miR-376b-3p whose expression were significantly changed glioma samples. Furthermore, investigated clinical samples cell lines using qRT-PCR analysis correlation between them RNA immunoprecipitation dual-luciferase reporter. The underlying mechanisms SNHG1 also immunohistochemistry...

Abstract There is growing evidence that the long non-coding RNAs(lncRNAs) play an important role in biological behaviors of glioblastoma cells. In this study, we elucidated function and possible effect molecular mechanisms lncRNA-Linc-00313 on cells as well UPF1 a RNA-binding protein to enhance its stability. Here, used qRT-PCR western blot measure expression, cell Transfection disrupt expression genes, viability analysis, quantization apoptosis, migration, invasion assays, Reporter vectors...

Tertiary structure-based RNA design plays a crucial role in synthetic biology and therapeutics. While existing methods have explored structure-to-sequence mappings, they focus solely on structures overlook the of complex-level information, which is for effective design. To address this limitation, we propose Complex-Aware tertiary Design model, CARD, that integrates information to enhance sequence be specific, our method incorporates protein features extracted by language model (e.g.,...

MicroRNAs are currently considered as an active and rapidly evolving area for the treatment of tumors. In this study, we elucidated biological significance miR-330 in glioblastoma stem cells (GSCs) well possible molecular mechanisms. SH3GL2 is mainly distributed central nervous system to be a tumor suppressor many present identified potential regulator found that it was inversely correlated with expression GSCs which were isolated from U87 cell lines. The enhanced cellular proliferation,...