A5021734302- Neuroscience and Neuropharmacology Research
- Tryptophan and brain disorders
- Stress Responses and Cortisol
- Receptor Mechanisms and Signaling
- Neurogenesis and neuroplasticity mechanisms
- Adipose Tissue and Metabolism
- Mitochondrial Function and Pathology
- Cell Image Analysis Techniques
- Treatment of Major Depression
- Microtubule and mitosis dynamics
- Neurobiology and Insect Physiology Research
- Lipid Membrane Structure and Behavior
- MicroRNA in disease regulation
- Genetic Neurodegenerative Diseases
- Neuroinflammation and Neurodegeneration Mechanisms
- Child and Adolescent Psychosocial and Emotional Development
- Genetics and Neurodevelopmental Disorders
- Medical Imaging and Analysis
- Axial and Atropisomeric Chirality Synthesis
- Cellular transport and secretion
- Cellular Mechanics and Interactions
- RNA Interference and Gene Delivery
- RNA Research and Splicing
- Zebrafish Biomedical Research Applications
- Proteoglycans and glycosaminoglycans research
Polish Academy of Sciences
2016-2025
Instytut Biologii Doświadczalnej im. Marcelego Nenckiego
2016-2025
Jagiellonian University
2018
University of Warsaw
2016
Institute of Biochemistry and Biophysics, Polish Academy of Sciences
2016
University of Virginia
2016
Synaptic cell adhesion molecules regulate signal transduction, synaptic function, and plasticity. However, their role in neuronal interactions with the extracellular matrix (ECM) is not well understood. Here we report that CD44, a transmembrane receptor for hyaluronan, modulates High-resolution ultrastructural analysis showed CD44 was localized at mature synapses adult brain. The reduced expression of affected excitatory transmission primary hippocampal neurons, simultaneously modifying...
Major depressive disorder is a complex disease resulting from aberrant synaptic plasticity that may be caused by abnormal serotonergic signaling. Using combination of behavioral, biochemical, and imaging methods, we analyze 5-HT7R/MMP-9 signaling dendritic spine in the hippocampus mice treated with selective 5-HT7R agonist (LP-211) model chronic unpredictable stress (CUS)-induced depressive-like behavior. We show acute activation induces behavior an MMP-9-dependent manner post mortem brain...
Abstract S-palmitoylation, a dynamic post-translational modification, has long been suggested to play pivotal role in synaptic plasticity, learning, and memory. However, its precise impact on proteins function remains unclear. In this study, we show that acute protein depalmitoylation the hippocampus differentially affects short- long-term depending synapse type. Strikingly, also reprograms neuronal spiking timing following associative network activation. Our research identifies pre-...
Ketamine is an N-methyl-d-aspartate receptor antagonist that has gained wide attention as a potent antidepressant. It also been recently reported to have prophylactic effects in animal models of depression and anxiety. Alterations neuroplasticity different brain regions; such the hippocampus; prefrontal cortex; amygdala; are hallmark stress-related disorders; changes may endure beyond treatment symptoms. The present study investigated whether injection ketamine on structural plasticity mice...
The observation and analysis of dendritic spines morphological changes poses a major challenge in neuroscience studies. alterations their density and/or morphology are indicators the cellular processes involved neural plasticity underlying learning memory, symptomatic neuropsychiatric disorders. Despite ongoing intense investigations imaging approaches, relationship between spine synaptic function is still unknown. existing quantitative analyses difficult to perform require extensive user...
Accurate and effective dendritic spine segmentation from the dendrites remains as a challenge for current neuroimaging research community. In this article, we present new method (2dSpAn) 2-d segmentation, classification analysis of structural/plastic changes hippocampal spines. A user interactive with convolution kernels is designed to segment spines dendrites. Formal morphological definitions are presented describe key attributes related shape segmented Spines automatically classified into...
The common approach in morphological analysis of dendritic spines mammalian neuronal cells is to categorize into subpopulations based on whether they are stubby, mushroom, thin, or filopodia shaped. corresponding cellular models synaptic plasticity, long-term potentiation, and depression associate the strength with either spine enlargement shrinkage. Although a variety automatic segmentation feature extraction methods were developed recently, no approaches allowing for an unbiased...
Alterations in social behavior are core symptoms of major developmental neuropsychiatric diseases such as autism spectrum disorders or schizophrenia. Hence, understanding their molecular and cellular underpinnings constitutes the research task. Dysregulation global gene expression program developing brain leads to modifications a number neuronal connections, synaptic strength shape, causing unbalanced plasticity, which may be important substrate pathogenesis neurodevelopmental disorders,...
Although sex differences in the brain are prevalent, knowledge about mechanisms underlying sex-related effects on normal and pathological functioning is rather poor. It known that female male brains differ size connectivity. Moreover, those related to neuronal morphology, synaptic plasticity, molecular signaling pathways. Among different processes assuring proper synapse functions posttranslational modifications, among them, S-palmitoylation (S-PALM) emerges as a crucial mechanism regulating...
Clinical and preclinical studies show evidence that chronic stress or nutritional deficits in dietary zinc (Zn) intake may be risk factors for developing major depressive disorder (MDD). Furthermore, there possible links between low serum Zn levels development of treatment-resistant depression. In the present work, we combined restraint (CRS) a low-zinc diet (ZnD) mice carried out set behavioral biochemical studies. The were treated with four different antidepressant compounds, namely,...
Stress resilience is the ability of neuronal networks to maintain their function despite stress exposure. Using a mouse model we here investigate phenomenon. To assess resilient and anhedonic behavioral phenotypes developed after induction chronic unpredictable stress, quantitatively characterized structural functional plasticity excitatory synapses in hippocampus using combination proteomic, electrophysiological, imaging methods. Our results indicate that an active multifactorial process...
Stress resilience is the ability of neuronal networks to maintain their function despite stress exposure. Using a mouse model we investigate phenomenon. To assess resilient and anhedonic behavioral phenotypes developed after induction chronic unpredictable stress, quantitatively characterized structural functional plasticity excitatory synapses in hippocampus using combination proteomic, electrophysiological, imaging methods. Our results indicate that an active multifactorial process...
Abstract Stress resilience is the ability of neuronal networks to maintain their function despite stress exposure. Using a mouse model we here investigate phenomenon. To assess resilient and anhedonic behavioral phenotypes developed after induction chronic unpredictable stress, quantitatively characterized structural functional plasticity excitatory synapses in hippocampus using combination proteomic, electrophysiological, imaging methods. Our results indicate that an active multifactorial...
Abstract Stress resilience is an ability of neuronal networks to maintain their function despite the stress exposure. In this study, we investigate whether actively developed dynamic process in adult mice. order assess resilient and anhedonic behavioral phenotypes after induction chronic unpredictable stress, quantitatively characterized structural functional plasticity excitatory synapses hippocampus using a combination proteomic, electrophysiological, imaging methods. Our results indicate...
Abstract The common approach in morphological analysis of dendritic spines is to categorize into subpopulations based on whether they are stubby, mushroom, thin, or filopodia. Corresponding cellular models synaptic plasticity, long-term potentiation, and depression associate strength with either spine enlargement shrinkage. Although a variety automatic segmentation feature extraction methods were developed recently, no approaches allowing for an unbiased distinction between detailed...
Abstract Disturbances of gene expression patterns that occur during brain development can severely affect signal transmission, connectivity, and plasticity—key features underlie memory formation storage in neurons. Abnormalities at the molecular level manifest as changes structural functional plasticity dendritic spines harbor excitatory synapses. This lead to such developmental neuropsychiatric conditions Autism spectrum disorders, intellectual disabilities, schizophrenia. The present study...