A5013430940- Plant Micronutrient Interactions and Effects
- Plant Stress Responses and Tolerance
- Aluminum toxicity and tolerance in plants and animals
- Plant Molecular Biology Research
- Plant Reproductive Biology
- Photosynthetic Processes and Mechanisms
- Rice Cultivation and Yield Improvement
- Plant nutrient uptake and metabolism
- GABA and Rice Research
- Plant Gene Expression Analysis
- Plant tissue culture and regeneration
- Trace Elements in Health
- Cassava research and cyanide
- Chromosomal and Genetic Variations
- Plant-Microbe Interactions and Immunity
- Heavy metals in environment
- Plant Disease Resistance and Genetics
- Transgenic Plants and Applications
- Legume Nitrogen Fixing Symbiosis
- Polysaccharides and Plant Cell Walls
- Plant Genetic and Mutation Studies
- Plant Virus Research Studies
- Genomics and Phylogenetic Studies
- Genomics, phytochemicals, and oxidative stress
- Agricultural Science and Fertilization
Rural Development Administration
2024
National Academy of Agricultural Science
2024
Institute for Basic Science
2018-2023
National Institute of Agricultural Science and Technology
2022-2023
Dartmouth College
2009-2021
Daegu Gyeongbuk Institute of Science and Technology
2017
Dartmouth Hospital
2010-2012
Kyung Hee University
2010-2011
Pohang University of Science and Technology
1999-2010
University of Massachusetts Amherst
2009
Summary We have produced 22 090 primary transgenic rice plants that carry a T‐DNA insertion, which has resulted in 18 358 fertile lines. Genomic DNA gel‐blot and PCR analyses shown approximately 65% of the population contains more than one copy inserted T‐DNA. Hygromycin resistance tests revealed contain an average 1.4 loci inserts. Therefore, it can be estimated 25 700 taggings been generated. The binary vector used insertion contained promoterless β‐glucuronidase (GUS) reporter gene with...
Uptake and translocation of micronutrients are essential for plant growth. These also important food components. We generated transgenic rice plants over-expressing OsIRT1 to evaluate its functional roles in metal homeostasis. Those showed enhanced tolerance iron deficiency at the seedling stage. In paddy fields, this over-expression caused architecture be altered. addition, those were sensitive excess Zn Cd, indicating that transports metals. As expected, zinc contents elevated shoots,...
Abstract Uptake and translocation of metal nutrients are essential processes for plant growth. Graminaceous species release phytosiderophores that bind to Fe3+; these complexes then transported across the plasma membrane. We have characterized OsYSL15, one rice (Oryza sativa) YS1-like (YSL) genes strongly induced by iron (Fe) deficiency. The OsYSL15 promoter fusion β-glucuronidase showed it was expressed in all root tissues when Fe limited. In low-Fe leaves, became active except epidermal...
The most widespread dietary problem in the world is mineral deficiency. We used nicotianamine synthase ( NAS ) gene to increase contents rice grains. Nicotianamine (NA) a chelator of metals and key component metal homeostasis. isolated activation-tagged mutant lines which expression gene, OsNAS3 , was increased by introducing 35S enhancer elements. Shoots roots plants OsNAS3-D1 accumulated more Fe Zn. Seeds from our grown on paddy field contained elevated amounts (2.9-fold), Zn (2.2-fold),...
Abstract Cadmium (Cd) is a widespread soil pollutant; thus, the underlying molecular controls of plant Cd tolerance are substantial interest. A screen for wheat (Triticum aestivum) genes that confer to hypersensitive yeast strain identified Heat shock transcription factor A4a (HsfA4a). Ta HsfA4a most similar class A4 Hsfs from monocots. The closely related rice (Oryza sativa) homolog, Os HsfA4a, conferred in yeast, as did but second HsfA4d, not. was enhanced plants expressing and decreased...
Rice contains several MADS box genes. It has been demonstrated previously that one of these genes, OsMADS1 (for Oryza sativa gene1), is expressed preferentially in flowers and causes early flowering when ectopically tobacco plants. In this study, we ectopic expression rice also results flowering. To further investigate the role during flower development, generated transgenic plants expressing altered genes contain missense mutations domain. There was no visible alteration vegetative stage....
Abstract P1B-type heavy-metal ATPases (HMAs) are transmembrane metal-transporting proteins that play a key role in metal homeostasis. Despite their importance, very little is known about functions monocot species. We report the characterization of rice (Oryza sativa) OsHMA9, member ATPase family. Semiquantitative reverse transcription-polymerase chain reaction analyses seedlings showed OsHMA9 expression was induced by high concentration copper (Cu), zinc (Zn), and cadmium. also determined,...
Abstract Rice blast, caused by the fungus Magnaporthe oryzae, is one of most devastating diseases rice. To understand molecular basis Pi5-mediated resistance to M. we cloned (R) gene at this locus using a map-based cloning strategy. Genetic and phenotypic analyses 2014 F2 progeny from mapping population derived cross between IR50, susceptible rice cultivar, RIL260 line carrying Pi5 enabled us narrow down 130-kb interval. Sequence analysis genomic region identified two candidate genes, Pi5-1...
Summary We generated rice lines with increased content of nicotianamine (NA), a key ligand for metal transport and homeostasis. This was accomplished by activation tagging synthase 2 ( OsNAS2 ). Enhanced expression the gene resulted in elevated NA levels, greater Zn accumulations improved plant tolerance to deficiency. Expression Zn‐uptake genes those biosynthesis phytosiderophores (PS) were transgenic plants. suggests that higher amount led exudation PS from roots, as well stimulated...
Because micronutrients in human diets ultimately come from plant sources, malnutrition of essential minerals is a significant public health concern. By increasing the expression nicotianamine synthase (NAS), we fortified level bioavailable iron rice seeds. Activation deficiency-inducible OsNAS2 resulted rise Fe content (3.0-fold) mature Its ectopic also increased that content. Enhanced led to higher tolerance deficiency and better growth under elevated pH. Mice fed with OsNAS2-D1 seeds...
Summary Nitrogen (N) is a major factor for plant development and productivity. However, the application of nitrogenous fertilizers generates environmental economic problems. To cope with increasing global food demand, rice varieties high nitrogen use efficiency (NUE) indispensable reducing issues achieving sustainable agriculture. Here, we report that concomitant activation ( Oryza sativa ) Ammonium transporter 1;2 OsAMT1;2 Glutamate synthetase 1 (OsGOGAT1) genes leads to increased tolerance...
Abstract Increased grain yield will be critical to meet the growing demand for food, and could achieved by delaying crop senescence. Here, via quantitative trait locus (QTL) mapping, we uncover genetic basis underlying distinct life cycles senescence patterns of two rice subspecies, indica japonica . Promoter variations in Stay-Green ( OsSGR ) gene encoding chlorophyll-degrading Mg ++ -dechelatase were found trigger higher earlier induction , which accelerated cultivars. The -type promoter...
Abstract Nitrogen (N) is a major limiting factor affecting crop yield in unfertilized soil. Thus, cultivars with high N use efficiency (NUE) and good grain protein content (GPC) are needed to fulfill the growing food demand reduce environmental burden. This especially true for rice (Oryza sativa L.) that cultivated input of fertilizer primary staple more than half global population. Here, we report asparagine synthetase 1 (OsASN1) required contents under both N-sufficient (conventional paddy...
Rice (Oryza sativa L.) is a daily staple food crop for more than half of the global population and improving productivity an important task to meet future demands expanding world population. The application nitrogen (N) fertilization improved rice growth in world, but excess use causes environmental economic issues. One main goals breeding reducing N while maintaining productivity. Therefore, enhancing efficiency (NUE) essential development sustainable agriculture has become urgently needed....
SUMMARY Zinc (Zn) is essential for normal plant growth and development. The Zn‐regulated transporter, iron‐regulated transporter (IRT)‐like protein (ZIP) family members are involved in Zn transport cellular homeostasis throughout the domains of life. In this study, we have characterized four ZIP transporters from Arabidopsis thaliana (IRT3, ZIP4, ZIP6, ZIP9) to better understand their functional roles. proteins can restore defect a yeast uptake mutant upregulated under deficiency. Single...
Abstract The genomic clone encoding an α-tubulin,OsTubA1, has been isolated from rice (Oryza sativa L.). gene consists of four exons and three introns. RNA-blot analysis showed that the is strongly expressed in actively dividing tissues, including root tips, young leaves, flowers. Analysis chimeric fusions between OsTubA1and β-glucuronidase (GUS) revealed intron 1 was required for high-level GUS expression corresponding with normal pattern ofOsTubA1. Fusion constructs lacking more staining...