A5020232353- Spectroscopy and Quantum Chemical Studies
- Photoreceptor and optogenetics research
- Ethics in medical practice
- Photochemistry and Electron Transfer Studies
- Photosynthetic Processes and Mechanisms
- Advanced Fluorescence Microscopy Techniques
- Mitochondrial Function and Pathology
- Metabolism and Genetic Disorders
- Advanced Electron Microscopy Techniques and Applications
- SARS-CoV-2 and COVID-19 Research
- Palliative Care and End-of-Life Issues
- Anesthesia and Sedative Agents
- Dementia and Cognitive Impairment Research
- Vaccine Coverage and Hesitancy
- Geriatric Care and Nursing Homes
- Cell Image Analysis Techniques
- Spectroscopy Techniques in Biomedical and Chemical Research
- Photochromic and Fluorescence Chemistry
- Nuclear reactor physics and engineering
- Health Promotion and Cardiovascular Prevention
- Clinical Nutrition and Gastroenterology
- Health Systems, Economic Evaluations, Quality of Life
- Autoimmune and Inflammatory Disorders Research
- Diabetes Management and Education
- Cystic Fibrosis Research Advances
Janelia Research Campus
2019-2024
Howard Hughes Medical Institute
2019-2024
Maria College
2019-2022
Albany College of Pharmacy and Health Sciences
2022
10X Genomics (United States)
2021
New York State Department of Health
2005-2018
University of California, Berkeley
2012-2018
Berkeley College
2015
University at Albany, State University of New York
2014
Consumers Union
1991-1992
Within cells, the spatial compartmentalization of thousands distinct proteins serves a multitude diverse biochemical needs. Correlative super-resolution (SR) fluorescence and electron microscopy (EM) can elucidate protein relationships to global ultrastructure, but has suffered from tradeoffs structure preservation, retention, resolution, field view. We developed platform for three-dimensional cryogenic SR focused ion beam-milled block-face EM across entire vitreously frozen cells. The...
Cytotoxic T lymphocytes (CTLs) and natural killer cells kill virus-infected tumor through the polarized release of perforin granzymes. Perforin is a pore-forming toxin that creates lesion in plasma membrane target cell which granzymes enter cytosol initiate apoptosis. Endosomal sorting complexes required for transport (ESCRT) proteins are involved repair small wounds. We found ESCRT were precisely recruited to sites CTL engagement immediately after release. Inhibition machinery...
Abstract To coordinate cellular physiology, eukaryotic cells rely on the rapid exchange of molecules at specialized organelle–organelle contact sites 1,2 . Endoplasmic reticulum–mitochondrial (ERMCSs) are particularly vital communication hubs, playing key roles in signalling molecules, lipids and metabolites 3,4 ERMCSs maintained by interactions between complementary tethering surface each organelle 5,6 However, due to extreme sensitivity these membrane interfaces experimental perturbation...
Femtosecond spectroscopy has revealed coherent wave packet motion time and again, but the question as to whether these coherences are necessary for reactivity or merely a consequence of experiment remained open. For diatomic systems in gas phase, such sodium iodide, dimensionality system requires coordinated atomic along reaction coordinate. Coherent dynamics also readily observed condensed-phase multidimensional chromophores proteins solvated charge transfer dimers. Is precisely...
Photochemical reactions are mediated by conical intersections (CI), which difficult to directly probe and characterize. To gain insight into CIs, two-dimensional femtosecond stimulated Raman spectroscopy (2D-FSRS) is used examine a model excited-state charge-transfer (CT) complex consisting of an electron donor, tetramethylbenzene (TMB), acceptor, tetracyanoquinodimethane (TCNQ). Following impulsive excitation, the transient absorption reveals large-amplitude wave packet motion along...
Two-dimensional femtosecond stimulated Raman spectroscopy (2D-FSRS) is used to probe the structural evolution of a modified cyclohexadiene as it undergoes photoinduced ring opening reaction. Analysis excited state vibrational data reveals oscillations center frequencies and amplitudes 21 high frequency modes. These in properties are due anharmonic couplings between finger print modes impulsively driven low molecular distortions state. The largest couplings, with intrinsic oscillation...
Azobenzenes are used in many applications because of their robust and reversible light induced trans cis isomerization about the NN bond, but mechanism this ultrafast reaction has not been conclusively defined. Addressing problem we have Femtosecond Stimulated Raman Spectroscopy (FSRS) to determine structural transients → photoisomerization azobenzene derivative, 4-nitro-4′-dimethylamino-azobenzene (NDAB). Key marker modes, such as 1570/1590 cm−1 NO2 stretch 1630 C–N(Me)2 stretch, enable...
Interfacial electron transfer between sensitizers and semiconducting nanoparticles is a crucial yet poorly understood process. To address this problem, we have used transient absorption (TA) femtosecond stimulated Raman spectroscopy (FSRS) to investigate the photoexcited dynamics of series triphenylamine–coumarin dye/TiO2 conjugates. The TA decay multiexponential, spanning time scales from 100 fs ps, while characteristic spectrum radical cation decays biexponentially with dominant ∼3 ps...
A comparison between a Fabry-Pérot etalon filter and conventional grating for producing the picosecond (ps) Raman pump pulses femtosecond stimulated spectroscopy (FSRS) is presented. It shown that of equal energy produces signals twice as large while suppressing electronically resonant background signal. The time asymmetric profile etalon-generated pulse to be responsible both these observations. theoretical discussion presented which quantitatively supports this hypothesis. concluded...
Azobenzenes are versatile photoswitches that find application in optical memory, light-driven motors, and molecular gating. Despite many studies, the details of their light induced trans to cis isomerization still debated. To inform this discussion we probed low frequency skeletal motions an azobenzene derivative, 4-nitro-4'-dimethylamino-azobenzene (NDAB), with resonant impulsive stimulated Raman spectroscopy (RISRS). Four previously unobserved modes at 14, 47, 150, 201 cm(-1) were found....
The time-resolved femtosecond stimulated Raman spectra (FSRS) of a charge transfer (CT) excited noncovalent complex tetracyanoethylene:1-chloronaphthalene (TCNE:ClN) in dichloromethane (DCM) is reported with 40 fs time resolution. In the frequency domain, five FSRS peaks are observed frequencies 534, 858, 1069, 1392, and 1926 cm-1. most intense at 534 1392 cm-1 correspond to fundamentals while features attributed difference frequency, an overtone combination fundamentals, respectively....
Exciton mobility is crucial to organic photovoltaic (OPV) efficiency, but accurate, quantitative measures and therefore precise understanding of this process are currently lacking. Here, we exploit the unique capabilities femtosecond stimulated Raman spectroscopy (FSRS) disentangle signatures bulk interfacial donor response in a heterojunction composed poly[2-methoxy-5-(3′,7′-dimethyloctyloxy)-1,4-phenylenevinylene] (MDMO-PPV) phenyl-C61-butyric acid methyl ester (PCBM). Surprisingly, find...
Abstract Structured illumination microscopy (SIM) is widely used for fast, long-term, live-cell super-resolution imaging. However, SIM images can contain substantial artifacts if the sample does not conform to underlying assumptions of reconstruction algorithm. Here we describe a simple, easy implement, process that be combined with any algorithm alleviate many common and briefly discuss possible extensions.
We live in a time when our knowledge and actions don't always match up.In this case, the of value
To coordinate cellular physiology, eukaryotic cells rely on the inter-organelle transfer of molecules at specialized organelle-organelle contact sites 1,2 . Endoplasmic reticulum-mitochondria (ERMCSs) are particularly vital communication hubs, playing key roles in exchange signaling molecules, lipids, and metabolites 3 ERMCSs maintained by interactions between complementary tethering surface each organelle 4,5 However, due to extreme sensitivity these membrane interfaces experimental...
Recent studies have pointed to the large and increasingly complex issues surrounding dementia in American society general health care particular. The initial foray into federal policy arena, National Alzheimer’s Project Act, is a good first step but remains limited scope resources. Seeing need for greater effort, thirty-three states convened advisory groups published their own plans coordinating state-level activity address disease across service systems. This article examines current state...
In Brief From 1997 through 1999, a total of 365 diabetes screening and awareness events targeting high-risk populations were held throughout New York State. These planned implemented by community-based coalitions that received funding from the state's Diabetes Control Program. The American Association's risk questionnaire was administered, those individuals identified as high capillary blood glucose test. Screened with readings above cut-off value (140 mg/dl or 110 if fasting) referred to...
Patients with Alzheimer’s disease and other types of dementia acute medical problems, who have lost capacity are without advance directives, at risk being over treated inhospitals. To deal this growing demographic ethical crisis, patients need to plan for their future care while they do so. This article will examine the role each member triad how empower patient participate in planning care. A case be made that physicians same professional disclosure obligations as all capable terminal...
Abstract Living cells function through the spatial compartmentalization of thousands distinct proteins serving a multitude diverse biochemical needs. Correlative super-resolution (SR) fluorescence and electron microscopy (EM) has emerged as pathway to directly view nanoscale protein relationships underlying global ultrastructure, but traditionally suffered from tradeoffs structure preservation, retention, resolution, field view. We developed platform for three-dimensional correlative...
A program has been developed for use on a digital computer which determines the temperature and velocity profiles in shallow body of water resulting from discharge condenser cooling power plant. The is based theory turbulent jets considers mass, momentum energy exchanges between jet, surrounding atmosphere. temperature, local current, wind speed shape bottom are taken into account along with parameters describing jet conditions at outfall. There two coefficients relating to vertical...