A5060052542- Sphingolipid Metabolism and Signaling
- Cellular transport and secretion
- Lipid metabolism and biosynthesis
- Endoplasmic Reticulum Stress and Disease
- Ion channel regulation and function
- Photosynthetic Processes and Mechanisms
- Autophagy in Disease and Therapy
- Ion Channels and Receptors
- Lipid Membrane Structure and Behavior
- Erythrocyte Function and Pathophysiology
- Adipose Tissue and Metabolism
- Calcium signaling and nucleotide metabolism
- Mitochondrial Function and Pathology
- Peroxisome Proliferator-Activated Receptors
- Neurobiology and Insect Physiology Research
- Advanced Fluorescence Microscopy Techniques
- Toxin Mechanisms and Immunotoxins
- Numerical Methods and Algorithms
- Digital Filter Design and Implementation
- Advanced Electron Microscopy Techniques and Applications
- Pancreatic function and diabetes
- Microbial Metabolic Engineering and Bioproduction
- Lipid metabolism and disorders
- Hereditary Neurological Disorders
- Wnt/β-catenin signaling in development and cancer
St. Jude Children's Research Hospital
2023-2024
Janelia Research Campus
2018-2021
Howard Hughes Medical Institute
2017-2021
National Yang Ming University Hospital
2019
The University of Texas Southwestern Medical Center
2012-2018
Southwestern Medical Center
2013-2017
Taipei Veterans General Hospital
2014
National Yang Ming Chiao Tung University
2014
National Taiwan University
2000-2013
Soochow University
2012
Endoplasmic reticulum (ER)-plasma membrane (PM) junctions are highly conserved subcellular structures. Despite their importance in Ca(2+) signaling and lipid trafficking, the molecular mechanisms underlying regulation functions of ER-PM remain unclear. By developing a genetically encoded marker that selectively monitors junctions, we found connection between ER PM was dynamically regulated by signaling. Elevation cytosolic triggered translocation E-Syt1 to enhance ER-to-PM connection. This...
Within cells, the spatial compartmentalization of thousands distinct proteins serves a multitude diverse biochemical needs. Correlative super-resolution (SR) fluorescence and electron microscopy (EM) can elucidate protein relationships to global ultrastructure, but has suffered from tradeoffs structure preservation, retention, resolution, field view. We developed platform for three-dimensional cryogenic SR focused ion beam-milled block-face EM across entire vitreously frozen cells. The...
Lipid droplets (LDs) are neutral lipid storage organelles that transfer lipids to various including peroxisomes. Here, we show the hereditary spastic paraplegia protein M1 Spastin, a membrane-bound AAA ATPase found on LDs, coordinates fatty acid (FA) trafficking from LDs peroxisomes through two interrelated mechanisms. First, Spastin forms tethering complex with peroxisomal ABCD1 promote LD–peroxisome contact formation. Second, recruits membrane-shaping ESCRT-III proteins IST1 and CHMP1B via...
Curcumin is a principal compound of turmeric, commonly used to treat tumors and other diseases. However, its anti-cancer activity in human acute monocytic leukemia THP-1 cells not clear. This study aimed the effect action curcumin on cells. parental PMA-treated cells, were as vitro models evaluate mechanism curcumin. Apoptosis evaluated by WST-1, flow cytometry Western blotting. MAPK inhibitors further confirm molecular curcumin-induced cell apoptosis. induced apoptosis shown viability,...
The endoplasmic reticulum (ER) Ca2+ sensor STIM1 forms oligomers and translocates to ER–plasma membrane (PM) junctions activate store-operated entry (SOCE) after ER depletion. also interacts with EB1 dynamically tracks microtubule (MT) plus ends. Nevertheless, the role of STIM1–EB1 interaction in regulating SOCE remains unresolved. Using live-cell imaging combined a synthetic construct approach, we found that binding constitutes trapping mechanism restricting targeting ER–PM junctions. We...
Contact sites between lipid droplets and other organelles are essential for cellular energy homeostasis upon metabolic demands. Detection of these contact at the nanometer scale over time in living cells is challenging. We developed a tool kit detecting based on fluorogen-activated bimolecular complementation CONtact sites, FABCON, using reversible, low-affinity split fluorescent protein, splitFAST. FABCON labels with minimal perturbation to organelle interaction. Via we quantitatively...
Pancreatic adenocarcinoma, a desmoplastic disease, is the fourth leading cause of cancer-related death in Western world due, large part, to locally invasive primary tumor growth and ensuing metastasis. SPARC matricellular protein that governs extracellular matrix (ECM) deposition maturation during tissue remodeling, particularly, wound healing tumorigenesis. In present study, we sought determine mechanism by which lack host alters microenvironment enhances invasion metastasis an orthotopic...
RAS association domain family 4 (RASSF4) is involved in tumorigenesis and regulation of the Hippo pathway. In this study, we identify new functional roles RASSF4. First, discovered that RASSF4 regulates store-operated Ca2+ entry (SOCE), a fundamental signaling mechanism, by affecting translocation endoplasmic reticulum (ER) sensor stromal interaction molecule 1 (STIM1) to ER–plasma membrane (PM) junctions. It was further revealed formation ER–PM junctions tethering function extended...
Sphingosine 1-phosphate (S1P) is a platelet- and endothelial cell-released lysophospholipid that regulates various cellular functions through activating specific family of G protein-coupled receptors. Both platelet activation angiogenesis play important roles in cancer development, implying cells might encounter large amount S1P during these processes. Cancer cells, the meantime, may experience nutrient deprivation rely on autophagy for early development. Whether extracellular remains to be...
Endoplasmic reticulum–plasma membrane (ER–PM) junctions mediate crucial activities ranging from Ca 2+ signaling to lipid metabolism. Spatial organization of ER–PM may modulate the extent and location these cellular activities. However, morphology distribution are not well characterized. Using photoactivated localization microscopy, we reveal that contact area single is mainly oblong with dimensions ∼120 nm × ∼80 in HeLa cells. total internal reflection fluorescence microscopy structure...
AbstractLysophosphatidic acid (LPA) is a platelet-enriched bioactive lysophospholipid. By binding to its cognitive G protein-coupled receptors, which are encoded by endothelial differentiation genes (Edg), LPA regulates various cellular activities including proliferation, survival, and migration. Currently, little known about the influences of on autophagy, pivotal mechanism for cell survival during conditions starvation. Herein we present data indicating that attenuates starvation-induced...