A5082742046- Dermatology and Skin Diseases
- Asthma and respiratory diseases
- Allergic Rhinitis and Sensitization
- IL-33, ST2, and ILC Pathways
- Immunodeficiency and Autoimmune Disorders
- Psoriasis: Treatment and Pathogenesis
- MicroRNA in disease regulation
- DNA Repair Mechanisms
- Peroxisome Proliferator-Activated Receptors
- Cancer-related molecular mechanisms research
- Flavonoids in Medical Research
- melanin and skin pigmentation
- Exercise and Physiological Responses
- Food Allergy and Anaphylaxis Research
- Genomics, phytochemicals, and oxidative stress
- T-cell and B-cell Immunology
- Vitamin D Research Studies
- Cancer therapeutics and mechanisms
- Neurological Disorders and Treatments
- Glutathione Transferases and Polymorphisms
- Telomeres, Telomerase, and Senescence
- Otitis Media and Relapsing Polychondritis
- Ocular Surface and Contact Lens
- Immune Cell Function and Interaction
- Sarcoma Diagnosis and Treatment
Huashan Hospital
2020-2025
Fudan University
2020-2025
Kangda College of Nanjing Medical University
2023
Nanjing Medical University
2011-2023
Second Affiliated Hospital of Inner Mongolia Medical University
2015
Jiangsu Province Hospital
2011-2014
National Eye Institute
2006-2008
National Institutes of Health
2007
Microbial dysbiosis in the skin has been implicated pathogenesis of atopic dermatitis (AD); however, whether and how changes microbiome initiate inflammation, or vice versa, remains poorly understood. Here, we report that levels sebum its microbial metabolite, propionate, were lower on surface AD patients compared with those healthy individuals. Topical propionate application attenuated inflammation mice MC903-induced AD-like by inhibiting IL-33 production keratinocytes, an effect was...
Abstract Background Atopic dermatitis (AD) is a highly heterogeneous disease clinically and biologically. Serum biomarkers have been utilized for endotype identification the potential to be predictors treatment. Objectives To explore serum biomarker-based endotypes of Chinese patients with AD identify prediction efficacy dupilumab. Methods Sera from 125 moderate-to-severe 60 normal controls (NC) were analysed 24 cytokines/chemokines using magnetic Luminex assay. After received 16 weeks...
Phase 3 trials have demonstrated the efficacy and safety of abrocitinib for atopic dermatitis (AD), but real-world evidence remains limited. This study prospectively enrolled 117 moderate-to-severe AD patients at Huashan Hospital, Shanghai, China. Physician- patient-reported outcomes were evaluated multiple time points. Blood eosinophil counts, serum IgE, 24 cytokines/chemokines measured. Abrocitinib treatment led to rapid potent improvements in disease severity. At week 12, 74.3% 50.5%...
Dupilumab, an antibody against interleukin-4 receptor α, has demonstrated elegant efficacy and safety profiles in patients with moderate-to-severe atopic dermatitis (AD). However, the of dupilumab varies among AD patients, compared Caucasian population, data for Asian people, especially Chinese is very limited.To investigate a real-world single-centre prospective cohort.We enrolled 138 receiving treatment at Huashan Hospital, Shanghai, China this 16-week, single-centre, prospective,...
Lipids are the major components of skin barrier, mainly produced by keratinocytes and sebaceous glands. Previous studies on barrier dysfunction atopic dermatitis (AD) focus lipids from keratinocytes, whereas role gland-derived in AD has long been underrecognized.The sebum secreted surface patients was measured using Delfin Sebum Scale. collected Sebutape patches subjected for liquid chromatography tandem-mass spectrometry (LC-MS/MS) analysis. Multivariate data analysis applied to explore...
Background/Objective: Atopic dermatitis (AD) is a chronic inflammatory skin disease characterized by diverse clinical manifestations. However, variations in its presentations across different ages, genders, anatomical sites, and seasons remain incompletely understood. The objective was to explore the heterogeneities of AD using data from Chinese non-selective registration system. Methods: A prospective analysis conducted on 3829 patients enrolled Non-selective Registry for (CNRAD) at...
ABSTRACT Atopic dermatitis (AD) patients usually have elevated serum IgE that is thought inducing inflammation upon binding to allergen. However, the role of IgE‐producing B cells and other isotypes immunoglobulin, such as IgG, in AD are not clear rarely explored. This study aimed investigate particularly skin lesion AD. BCR repertoires were analysed using mRNA prepared from lesions peripheral blood mononuclear (PBMCs) non‐allergic healthy subjects. Single‐cell RNA sequencing data published...
Reactive oxygen species (ROS) are an important factor in the development of skin photodamage after ultraviolet A (UVA) radiation. flavonoid antioxidant, baicalin, can selectively neutralize super-oxide anion (O(2)(-)) while having no significant effect on (•)OH. Fibroblasts a key component dermis. In present study, we investigated protective effects baicalin human fibroblasts (HSFs) under UVA induced oxidative stress. Fluorescence microscopy and flow cytometry were used to assay...
Abstract Background Elderly atopic dermatitis (AD) is a newly defined subtype of AD stratified by age‐related clinical pictures. Objectives To analyse the features and molecular profile elderly patients in China, comparing with infantile, childhood adolescent/adult AD. Methods A total 1312 diagnosed Hanifin Rajka diagnostic criteria from Huashan Hospital, Shanghai, were divided into four groups age (2–18, 19–40, 41–60 >60 years), evaluated questionnaire physical examination. Serum IgE,...
We aimed to evaluate the influence of four SNPs in ERCC1 and ERCC2 on response cisplatin-based treatment clinical outcome patients with osteosarcoma. identified 186 osteosarcoma diagnosed between April 2009 2011 who were eligible for inclusion our study. Genotyping rs11615, rs3212986, rs2298881; rs1799793 rs13181 was conducted by a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay. By conditional logistic regression analysis, carrying CC genotypes rs11615...
Atopic dermatitis (AD) is recognized as a common inflammatory skin disease and frequently occurred in Asian Black individuals.Since the limitation of dataset associated with human severe AD, this study aimed to screen potential novel biomarkers involved mild AD.Expression profile data (GSE75890) were obtained from database Gene Expression Omnibus. Using limma package, differentially expressed genes (DEGs) between samples AD healthy control selected. Furthermore, function analysis was...
Objective To evaluate the effect of miRNA-23a silencing on ultraviolet B (UVB)-induced chronic photodamage to fibroblasts.Methods Fibroblasts from human foreskin were divided into four groups:blank control group receiving untreated,UVB UVB irradiation only,miRNA-23a transfected with a antagomir,UVB + transfection antagomir followed by irradiation.UVB was carried out once day for five consecutive days at dose 10 mJ/cm2.Three after last irradiation,SA-β-galactosidase staining performed detect...
Objective: Elderly atopic dermatitis (AD) is a newly identified subtype of AD. Whether specific diagnostic criteria are needed for elderly AD has been debated. This study aimed to propose and evaluate the sensitivity. Methods: A hospital-based was conducted. We screened clinical features 1312 patients with different ages in one cohort proposed set The were then validated another 223 clinically diagnosed by dermatologists specialized examine sensitivity compared other chi-square test....
Objective To estimate the influence of palmitic acid (PA) on proliferation and production inflammatory mediators by a human keratinocyte line HaCaT.Methods Cultured HaCaT cells were treated with PA eight concentrations (0-200 μmol/L) for 3-24 hours followed evaluation cell using counting kit-8.According to assay,four (75,100,125,150 selected used treat 24 hours,then,fluorescence-based immunohistochemical staining was performed observe nuclear translocation factor (NF)-κB p65,enzyme linked...
Objective To investigate the inhibitory effect of baicalin on ultraviolet A (UVA)-induced oxidative damage and apoptosis in human diploid fibroblasts (HDFs) its possible mechanism.Methods Diploid were isolated from healthy adult male foreskin tissue subjected to primary culture subculture.To determine working concentrations baicalin,CCK-8 assay was performed evaluate proliferation HDFs treated with various (3.125-100 mg/L) alone or combination UVA irradiation.Then,some divided into 5...
Objective To observe the effect of sirolimus,an autophagy enhancer,on premature senescence in fibroblasts induced by repeated exposure to a subtoxic dose ultraviolet B (UVB).Methods Skin from foreskin tissue healthy adolescents were classified into six groups:control group cultured Dulbecco's modified Eagles' medium (DMEM) containing 1％ calf serum,UVB receiving UVB irradiation only,sirolimus treated with sirolimus 10 mg/L (added after daily exchange culture medium),and three combined groups...
Th17 cells that derive from initial T are a newly discovered class of CD4+ different Th1 and Th2 cells. They secrete interleukin (IL)-17, IL-6, IL-22, tumor necrosis factor other cytokines, play an important role in mediating chronic inflammation, autoimmune diseases, etc. Recent studies have evidenced IL-17 posseses potent anti-HIV activity exerts crucial cytokine regulatory networks. In early HIV infection, there is significant reduction or depletion peripheral blood gastrointestinal...
Abstract The role of Th17 lymphocytes in immunopathogenic processes has been well established, although little is known about their mode action and other biological activities. Here, we prepared polarized populations TCR-transgenic (Tg) Th1 or cells specific against hen egg lysozyme (HEL) compared each line for its capacity to adoptively transfer ocular inflammation into Tg recipients expressing lens-associated HEL, as To establish line, naive CD4+ were cultured the presence HEL "cocktails"...