A5032461322- Force Microscopy Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Advanced Biosensing Techniques and Applications
- Muscle metabolism and nutrition
- Tryptophan and brain disorders
- Metabolomics and Mass Spectrometry Studies
- Biosimilars and Bioanalytical Methods
- Advanced biosensing and bioanalysis techniques
- Exercise and Physiological Responses
- Peptidase Inhibition and Analysis
- Mass Spectrometry Techniques and Applications
- Adipose Tissue and Metabolism
- Traumatic Brain Injury and Neurovascular Disturbances
- Chemical Reactions and Isotopes
- Traumatic Brain Injury Research
- Monoclonal and Polyclonal Antibodies Research
- S100 Proteins and Annexins
- Drug Transport and Resistance Mechanisms
- Ethics in Clinical Research
- Cancer Genomics and Diagnostics
- Liver Disease Diagnosis and Treatment
- Pharmacogenetics and Drug Metabolism
- Microfluidic and Capillary Electrophoresis Applications
- High Altitude and Hypoxia
- Pediatric Hepatobiliary Diseases and Treatments
Institute of Biomedical Chemistry
2014-2025
Russian Academy of Sciences
2014
Kidney diseases pose a serious healthcare problem because of their high prevalence, worsening patients' quality life, and mortality. Patients with kidney are often asymptomatic until disease progression starts. Expensive renal replacement therapy options, such as dialysis or transplant, required for end-stage disease. Early diagnosis pathology is crucial slowing down curbing further damage. This study aimed to analyze the features protein composition blood plasma in patients most common...
Proteomic and metabolomic research enables quantitation of the molecular profile athletes. Multiomic profiling was conducted using plasma samples collected from 18 male athletes performing aerobic activity (running) at high altitude. Metabolomic detected changes in levels 4-hydroxyproline, methionine, oxaloacetate, tyrosine during recovery period. Furthermore, proteomic revealed expression proteins contributing to function immune system, muscle damage, metabolic fitness performance, as well...
The development of many human disorders, including celiac disease (CD), is thought to be influenced by the microbiota gastrointestinal tract and its metabolites, according current research. This study's goal was provide a concise summary information on contribution intestinal CD pathogenesis, which actively addressed while examining reported pathogenesis (CD). We assumed that change in gluten tolerance formed under influence number different factors, genetic predisposition environmental...
An approach combining atomic force microscopy ( AFM ) fishing and mass spectrometry MS analysis to detect proteins at ultra‐low concentrations is proposed. Fishing out protein molecules onto a highly oriented pyrolytic graphite surface coated with polytetrafluoroethylene film was carried without application of an external electric field. After that they were visualized by identified . It found injection solution leads charge generation in the solution, potential within measuring cell...
Abstract: A method for detection and identification of core antigen hepatitis C virus (HCVcoreAg)-containing particles in the serum was proposed, with due account taken interactions proteotypic peptides Na + , K Cl - ions. The is based on a combination reversible biospecific atomic force microscopy (AFM)-fishing mass spectrometry (MS). AFM-fishing enables concentration, detection, counting protein complexes captured AFM chip surface, their subsequent MS identification. Biospecific...
Abstract: Atomic force microscopy (AFM) was applied to carry out direct and label-free detection of gp120 human immunodeficiency virus type 1 envelope glycoprotein as a target protein. This approach based on the AFM fishing from analyte solution using anti-gp120 aptamers immobilized chip count gp120/aptamer complexes that were formed surface. The comparison image contrasts fished against background antibodies images conducted. It shown an contrast protein/aptamer two-fold higher than...
Abstract Post-translational processing leads to conformational changes in protein structure that modulate molecular functions and change the signature of metabolic transformations immune responses. Some post-translational modifications (PTMs), such as phosphorylation acetylation, are strongly related oncogenic processes malignancy. This study investigated a PTM pattern patients with gender-specific ovarian or breast cancer. Proteomic profiling analysis cancer-specific patterns were performed...
Microwave radiation at 3.4–4.2 GHz frequency of the cytochrome P450 CYP102 A1 (BM3) solution was registered during lauric acid hydroxylation reaction. The microwave generation shown to occur following addition electron donor NADPH a system containing an enzyme and substrate. occurs for solutions with concentrations 10−8 10−9 М. effect elicited by aqueous observed first time. results obtained can be used elaborate new approach systems research, including studying mechanism interaction...
A highly sensitive method for the qualitative and quantitative determination of amino- carboxylic acids, as well a number urea methionine cycle metabolites in studied solutions, is presented. Derivatives (esterification) were obtained amino acids by their reaction solution 3 N hydrochloric acid n-butanol 15 min at 65 °C with phenol ethyl acetate 20 °C. Experimental work on individual was carried out using HPLC-MS/MS included creation library spectra analyzed compounds determination....
A method of atomic force microscopy-based fishing (AFM fishing) has been developed for protein detection in the analyte solution using a chip with an immobilized aptamer. This is based on biospecific target from bulk onto small AFM area aptamer to this used as molecular probe. Such aptamer-based approach allows increase image contrast compared antibody-based approach. Mass spectrometry analysis after identify proved complex formation. Use avoids interference antibody and peaks mass spectrum.
Dried blood spot (DBS) technology has become a promising utility for the transportation and storage of biological fluids aimed subsequent clinical analysis. The basis DBS method is adsorption components sample onto surface membrane carrier, followed by drying. After drying, molecular biosample (nucleic acids, proteins, metabolites) can be analyzed using modern omics, immunological, or genomic methods. In this work, we investigated safety proteins on carrier tryptic over time at different...