A5043458045- Advanced Proteomics Techniques and Applications
- Blood transfusion and management
- Adrenal Hormones and Disorders
- Neurological Disorders and Treatments
- S100 Proteins and Annexins
- Traumatic Brain Injury and Neurovascular Disturbances
- Advanced NMR Techniques and Applications
- Electrolyte and hormonal disorders
- Regulation of Appetite and Obesity
- Mass Spectrometry Techniques and Applications
- Stress Responses and Cortisol
- Pituitary Gland Disorders and Treatments
- Metabolomics and Mass Spectrometry Studies
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Enzyme Structure and Function
- Nicotinic Acetylcholine Receptors Study
- Ubiquitin and proteasome pathways
- Medical Imaging Techniques and Applications
- Iron Metabolism and Disorders
- Axon Guidance and Neuronal Signaling
- Biosensors and Analytical Detection
- Adipose Tissue and Metabolism
- Adrenal and Paraganglionic Tumors
- Erythrocyte Function and Pathophysiology
- Endoplasmic Reticulum Stress and Disease
Burdenko Neurosurgery Institute
2015-2024
Ministry of Health of the Russian Federation
2018-2023
Russian Academy of Sciences
1994
There is a problem of bacterial contamination autologous blood despite long-term experience intraoperative salvage and reinfusion.
Liquid chromatography-tandem mass spectrometry was used to analyze plasma proteins of volunteers (control) and patients with glioblastoma multiform (GBM). A database search pre-set a variable post-translational modification (PTM): phosphorylation, acetylation or ubiquitination. There were no significant differences between the control GBM groups regarding number protein identifications, sequence coverage PTMs. However, in plasma, we unambiguously observed decreased fraction...
The development of commercially available panels for human blood plasma screening via selected reaction monitoring (SRM) offers reliable, cost-efficient and highly-standardized discovery validation protein biomarkers. However, detection by SRM can be hampered interfering peptide fragment ions. To estimate the influence interference on detection, we performed different types sample preparation implemented measurements well-characterized targets approved US Food Drug Administration.We used...
In the present study, we explored technology of liquid chromatography-mass spectrometry (HPLC-MS/MS) for proteome analysis blood plasma patients with early chronic cerebral ischemia. Analysis mass-spectrometer data carried out in automatic mode using software Progenesis LS-MS. As a result this study identified 43 proteins. The differences group compared control 7 It was found that stages ischemia changes affect proteins related to immune system, system maintenance hemostasis and lipid...
The relative differences between post-translational modifications (PTM) of proteins in blood plasma samples patients with cerebral ischemia (CI) and healthy people were investigated using the method label-free comparative proteomic analysis based on technology tandem HPLC-MS/MS. For PTM detection we used multiple MS/MS search database Mascot for variable Progenesis LS-MS software. In CI samples, observed an increase proportion peptides such as phosphorylation serine, threonine, tyrosine,...
Delta-He the difference between hemoglobin content in reticulocytes and erytrocytes is a relatively new laboratory indicator that easily measured everyday practice. This parameter directly related to iron bioavailability for synthesis can reflect various conditions accompanied by cytokine expression including systemic inflammation.To analyze prospects practical application of delta assessment neurosurgical patients throughout in-hospital treatment.We analyzed complete blood counts (Sysmex...
Recently, deuterium MR spectroscopy (DMRS), has gained attention in bothclinical and preclinical settings for studying brain metabolism healthy volunteersand patients with neuro-oncological conditions. DMRS can surpass the results of thegold standard metabolic imaging technique, positron emission tomography (PET)with amino acids tracers, which are considered to be tumor proliferation markers. Inthis article, we consider potential utility deuterated assessingcellular akin PET tracers.The aim...
Autologous blood obtained during hardware reinfusion can be bacterially contaminated with skin microflora in most cases (up to 90%) regardless of the area surgical intervention. Increased volume washing solution for red cell management, white filtration and X-ray irradiation do not always provide complete decontamination.
BACKGROUND: The glucagon test (GT) is a promising alternative to the insulin hypoglycemia (IHT) in diagnosis of secondary adrenal insufficiency (SAI). AIM: To study feasibility using GT patients after craniospinal irradiation and determine cut-off value rule out SAI. METHODS: A total 28 (14 males 14 females) with median age 19 years (17; 23) who had undergone combination treatment (surgery, (35 Gy) boost tumor bed, polychemotherapy) extrapituitary brain tumors no later than 2 before...
Aim: to present a new method for assessing glucose catabolism in brain tissues of healthy volunteers and neurooncology patients. This is MR spectroscopy with resonance frequency deuterium (hydrogen isotope) called metabolic imaging - DMI. Material methods. We searched scientific papers PubMed Google Scholar indexing systems 2017–2022 publicatioin years. Keywords used: spectroscopy, DMI, DMV, PET, non-proton tumor metabolism, Warburg effect tumor, glucose/glucolytic flux/metabolism. Results....
The review considers problem of upper limit reference interval TSH that is discussed predominantly by endocrinologists out laboratory community. common values 0.4-4.0 mE/l combine as a matter fact two ranges: zone practically full wellbeing (0.4-2.5 mE/l) and latent pathology and/or high possibility development clinically significant hypothyroidism (2.5-4.0 mE/l). At one time, the proposal to diminish provoked many discussions on balance received no approval. However, unanimity concerning...
Introduction. The concept of blood management in relation to the surgical treatment diseases recommends correction iron defi ciency anemia (IDA) at prehospital stage. However, surgery is often performed for emergency indications, making it necessary carry out as soon possible. Aim – evaluate possibilities using delta hemoglobin (Delta-He) monitor effectiveness IDA therapy neurosurgical patients stage reduce time required prepare hospitalization. Materials and methods. A total 42 were treated...
Concentrations of 46 proteins have been determined in human blood plasma using PlasmaDeepDive™ MRM Panel ("Biognosys AG", Switzerland). 18 them were included into the group with higher concentrations, also identified by shotgun proteomic analysis. Based on literature data it is concluded that applicable for studies samples potential biomarkers various nervous system disorders.Opredeleny kontsentratsii belkov plazmy krovi cheloveka metodom monitoringa dissotsiativnykh perekhodov s...
BACKGROUND: The most of the current studies include patients who are different by etiology secondary adrenal insufficiency (SAI), or investigate SAI among other late effects radiation therapy. AIMS: To describe features and to select best method screening in adult followed complex treatment nonpituitary brain tumors childhood. MATERIALS AND METHODS: It was retrospective cross-sectional study. 31 after childhood 20 healthy volunteers were examined. Age sex ratio comparable between groups....