A5071970926- Electrospun Nanofibers in Biomedical Applications
- Tissue Engineering and Regenerative Medicine
- Mesenchymal stem cell research
- Periodontal Regeneration and Treatments
- Infectious Aortic and Vascular Conditions
- Sexual function and dysfunction studies
- Wound Healing and Treatments
- 3D Printing in Biomedical Research
- Hernia repair and management
- COVID-19 Clinical Research Studies
- Long-Term Effects of COVID-19
- Ovarian function and disorders
- Diabetic Foot Ulcer Assessment and Management
- Cardiac Structural Anomalies and Repair
- Pluripotent Stem Cells Research
- Genital Health and Disease
- Cancer Cells and Metastasis
- Blood transfusion and management
- Aortic Disease and Treatment Approaches
- Renal Transplantation Outcomes and Treatments
- Biomedical Text Mining and Ontologies
- Corneal Surgery and Treatments
- Xenotransplantation and immune response
- T-cell and B-cell Immunology
- Pressure Ulcer Prevention and Management
Biomedical Research Foundation of the Academy of Athens
2018-2024
General Hospital of Athens G. Genimatas
2022-2024
Academy of Athens
2014-2024
National and Kapodistrian University of Athens
2018-2020
Laiko General Hospital of Athens
2009-2020
Institute for Biotechnology and Bioengineering
2019
University of Lisbon
2019
Mesenchymal stem or stromal cells (MSCs) are a heterogeneous population that can be isolated from many tissues including umbilical cord Wharton's jelly (UC-WJ). Although initially limited in studies such as hematopoietic cell transplantation adjuvant, an increasing number of clinical trials consider MSCs potential anti-inflammatory regenerative medicine agent. It has been proposed creating repository would increase their availability for applications. The aim this study was to assess the...
Erectile dysfunction (ED) affects more than 30 million men; endothelial plays a significant role in EDs pathogenesis. The aim of this study was to administer mesenchymal stem cells (MSC) derived from adipose tissue and platelet lysate (PL) into patients with erectile dysfunction. This pilot enrolled eight diagnosed ED. Patients were suffering organic ED due diabetes melitus, hypertension, hypercholesterolaemia, Peyronie disease. distributed 2 groups. group A received (ADMSC) resuspended PL...
Background: The intraovarian administration of autologous platelet-rich plasma (PRP) acts beneficially for the stimulation follicle production in women presenting different forms ovarian dysfunction. This pilot study aimed to evaluate and provide significant data regarding efficacy PRP rejuvenate ovaries. Methods: A total 253 aged 22-56 years, were divided into five groups, based on their status. All participants signed informed consent current study. Blood sampling, preparation infusion...
Mesenchymal stromal cells (MSCs) are a mesodermal stem cell population, with known self-renewal and multilineage differentiation properties. In the last century, MSCs have been widely used in regenerative medicine tissue engineering approaches. initially were isolated from bone marrow aspirates, but currently identified great number of tissues human body. Besides their utilization medicine, possess significant immunoregulatory/immunosuppressive properties, through interaction innate adaptive...
Most public cord blood (CB) banks currently discard more than 80% of umbilical CB units not suitable for hemopoietic stem cell transplant due to low count. Although platelets, plasma, and red cells have been used experimental allogeneic applications in wound healing, corneal ulcer treatment, neonatal transfusion, no standard procedures their preparation defined internationally.A network 12 Spain, Italy, Greece, the UK, Singapore developed a protocol validate procedure routine production...
Abstract Human leukocyte antigens (HLA) represent one of the most polymorphic systems in humans, responsible for identification foreign and presentation immune responses. Therefore, HLA is considered to play a major role human disorders, donor‐recipient matching transplantation outcomes. This study aimed determine class I II alleles haplotypes Greek population. Moreover, comparative analysis haplotype frequencies found pooled European populations was also performed acquire better knowledge...
Background: Current esophageal treatment is associated with significant morbidity. The gold standard therapeutic strategies are stomach interposition or autografts derived from the jejunum and colon. However, severe adverse reactions, such as leakage, stenosis infection, accompany above treatments, which, most times, life threating. aim of this study was optimization a decellularization protocol in order to develop proper tissue engineered construct. Methods: Rat esophagi were obtained...
Computational methods were combined with an experimental setup in order to investigate the response of human umbilical cord stem cells 3D electrospun and printed scaffolds, when dynamically stimulated a bioreactor. Key parameters associated bioreactor working conditions computationally investigated using Comsol software use output for planned setup. Based on theoretical observations, influence inlet velocity, cell number, exposure time analyzed vitro adjusted accordingly. MSCs seeded...
Background: The current treatment of Erectile Dysfunction (ED) is mainly based on the use drugs that provide erections shortly after but they do not really treat problem. Stem cell therapy a novel with regenerative properties can possibly erectile dysfunction. Methods: Five patients disease were treated Adipose-Derived Cells (ADSCs) and Platelet Lysate Plasma (PLP). ADSCs obtained through abdominal liposuction PLP was prepared obtaining blood samples from peripheral veins. function evaluated...
Mesenchymal Stromal Cells (MSCs) from Wharton's Jelly (WJ) tissue express HLA-G, a molecule which exerts several immunological properties. This study aimed at the evaluation of HLA-G expression in MSCs derived vitrified WJ tissue.WJ samples were isolated human umbilical cords, with use VS55 solution and stored for 1 year -196 °C. After storage, was thawed isolated. Then, expanded until reaching passage 8, followed by estimation cell number, doubling time (CDT), population (PD) viability. In...
The gold standard vascular substitutes, used in cardiovascular surgery, are the Dacron or expanded polytetrafluoroethylene (ePTFE)-derived grafts. However, major adverse reactions accompany their use. For this purpose, decellularized human umbilical arteries (hUAs) may be proven as a significant source for development of small diameter conduits. aim study was evaluation decellularization protocol hUAs. To effect to hUAs, histological analysis performed. Then, native and hUAs were...
Each year, more than 800,000 vascular and cardiac surgeries are performed therefore, there is a great need for suitable material bioprosthetic operations. Porcine pericardium double-walled sac that covers the heart can be used in thoracic surgery.The aim of present study was to evaluate decellularization process biomechanical properties porcine pericardial tissue after treatment.A detergent based protocol pericardium. Histological analysis contact cytotoxicity assay were performed....
Abstract: Background: Multipotent Mesenchymal Stromal Cells (MSCs) are used in tissue engineering and regenerative medicine. The vitro isolation expansion of MSCs involve the use foetal bovine serum (FBS). However, many concerns have been raised regarding safety this product. In study, alternative additives derived either from peripheral or cord blood were tested as an FBS replacement. Methods: Platelet lysates (PL) for MSCs. levels growth factors (PB) (CB) PLs determined using Multiple...
The primary therapeutic strategy in cardiovascular disease is the coronary artery bypass surgery, which in- volves use of small diameter vascular grafts (<6 mm). Human umbilical arteries could be used as a source for development these grafts.The aim this study was decellularization human and evaluation their re- cellularization potential.Decellularization performed with detergent based protocol. Histological analysis order to determine effect decellularization. Then, recellularization by...
Peripheral arterial disease causes blood vessel dysfunction that requires surgical intervention. Current interventions employ synthetic or allogeneic vascular grafts, which offer biocompatible material solutions are not able to regenerate grow with the patient. Decellularized scaffolds have gained significant momentum in past few years, since they potential The aim of this study was investigate effects modified decellularization protocol on biocompatibility and immunogenicity rat abdominal...
Background: Recent findings have shown that the fibrin gel derived from cord blood units (CBUs) play a significant role in wound healing and tissue regeneration. The aim of this study was to standardize production process order allow for its regular use. Methods: CBUs (n = 200) were assigned 4 groups according their initial volume. Then, two-stage centrifugation protocol applied obtain platelet rich plasma (PRP). concentration platelets (PLTs), white cells (WBCs) red (RBCs) determined prior...
This study was conducted to examine the hypothesis that umbilical cord blood platelet lysate (UCB-PL) gel has a significant impact on healing rate of DFU. Μethods: In this open-labeled, randomized controlled trial, 110 patients were treatment with UCB-PL (UCB-PL group, n = 52) every three days for one month or dressing normal saline (control 58). All participants followed up 20 weeks post treatment. Ulcer surface area assessed imitoMeasure application at two, four, and six weeks, four...
The development of fully functional small diameter vascular grafts requires both a properly defined vessel conduit and tissue-specific cellular populations. Mesenchymal stromal cells (MSCs) derived from the Wharton's Jelly (WJ) tissue can be used as source for obtaining smooth muscle (VSMCs), while human umbilical arteries (hUAs) serve scaffold blood engineering.To develop VSMCs WJ-MSCs utilizing cord platelet lysate.WJ-MSCs were isolated expanded until passage (P) 4. according to criteria...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is responsible for the disease 2019 (COVID-19) pandemic, which was initiated in December 2019. COVID-19 characterized by a low mortality rate (< 6%); however, this percentage higher elderly people and patients with underlying disorders. mild to severe outcomes. Currently, several therapeutic strategies are evaluated, such as use of anti-viral drugs, prophylactic treatment, monoclonal antibodies, vaccination. Advanced cellular...