A5053007502- Analytical Chemistry and Chromatography
- Microfluidic and Capillary Electrophoresis Applications
- Chemical and Physical Properties in Aqueous Solutions
- Phytochemicals and Antioxidant Activities
- Crystallization and Solubility Studies
- Electrochemical Analysis and Applications
- Free Radicals and Antioxidants
- Surfactants and Colloidal Systems
- Computational Drug Discovery Methods
- Innovative Microfluidic and Catalytic Techniques Innovation
- Advancements in Transdermal Drug Delivery
- Tea Polyphenols and Effects
- Analytical Methods in Pharmaceuticals
- Electrochemical sensors and biosensors
- Essential Oils and Antimicrobial Activity
- Chemical Thermodynamics and Molecular Structure
- Protein purification and stability
- Biochemical Analysis and Sensing Techniques
- Crystallography and molecular interactions
- Microfluidic and Bio-sensing Technologies
- Drug Solubulity and Delivery Systems
- Metabolomics and Mass Spectrometry Studies
- Chromatography in Natural Products
- Spectroscopy and Chemometric Analyses
- Biochemical and biochemical processes
Institut de Biomedicina de la Universitat de Barcelona
2010-2024
Universitat de Barcelona
2015-2024
Government of Catalonia
2015-2024
Institute of Advanced Chemistry of Catalonia
2010-2014
Consejo Superior de Investigaciones Científicas
2008-2012
GTx (United States)
2009
Instituto de Investigaciones Químicas y Ambientales de Barcelona
2009
Unilever (Netherlands)
2007
Universitat de València
2002
Few regulators of phenylpropanoids have been identified in monocots having potential as biofuel crops. Here we demonstrate the role maize (Zea mays) R2R3-MYB factor ZmMYB31 control phenylpropanoid pathway. We determined its vitro consensus DNA-binding sequence ACC(T)/(A) ACC, and chromatin immunoprecipitation (ChIP) established that it interacts with two lignin gene promoters vivo. To explore a regulator other plants, regulation pathway was further investigated Arabidopsis thaliana....
Proanthocyanidins (PAs) or condensed tannins, a major group of dietary polyphenols, are oligomers and polymers flavan-3-ol flavan-3, 4-diols widely distributed in plant foods. Most literature data on PAs' metabolic fate deal with PAs that can be extracted from the food matrix by aqueous-organic solvents ( extractable proanthocyanidins). However, there no colonic fermentation non-extractable proanthocyanidins (NEPAs), which arrive almost intact to colon, mostly associated fibre (DF). The aim...
<p class="ADMETabstracttext">This commentary addresses data quality in equilibrium solubility measurement aqueous solution. Broadly discussed is the “gold standard” shake-flask (SF) method used to measure of ionizable drug-like molecules as a function pH. Many factors affecting are recognized. Case studies illustrating analysis both solution and solid state aspects presented. Coverage includes drug aggregation (sub-micellar, micellar, complexation), use mass spectrometry assess...
The non-extractable fraction of many fruit and vegetables contains putatively bioactive polyphenolic compounds that, in most cases, have not been well characterised structurally. Non-extractable proanthocyanidins (NEPA) a polymeric nature are part the dietary fibre food. Using liquid chromatography coupled to mass spectrometer equipped with an electrospray ionisation chamber triple quadrupole analyser for tandem analysis (HPLC–ESI–QqQ–MS/MS) techniques, we examine phenolic metabolites...
Grape antioxidant dietary fiber (GADF) is a phenolic-rich matrix. The aim of this work was to determine which phenolic compounds come into contact with colonic epithelial tissue after the ingestion GADF. By use HPLC-ESI-MS/MS techniques metabolites were detected in feces, cecal content, and from rats. Free (epi)catechin (EC) all three sources, more than 20 conjugated EC also feces. Fourteen microbially derived identified and/or tissue. These results show that during transit along digestive...
The internal standard capillary electrophoresis method (IS-CE) has been implemented in a novel sequential injection–capillary instrument for the high-throughput determination of acidity constants (pKa) regardless aqueous solubility, number pKa values, or structure. This comprises buffer creation system that automatically mixes within few seconds four reagents situ separation electrolyte with pH range 2–13, ionic strength 10–100 mM and organic solvent content from 0% to 40%. Combined 1.2...
The performance of the most common and also some other less CE buffers has been tested for pKa determination several types compounds (pyridine, amines, phenols). selected cover a pH ranging from 3.7 to 11.8. Whereas buffers, like acetic acid/acetate, BisTrisH+/BisTris, TrisH+/Tris, CHES/CHES-, CAPS/CAPS- can be used with all type analytes, others ammonium/ammonia, butylammonium/butylammonia, ethylammonium/ethylammonia, diethylammonium/diethylammonia, hydrogenphosphate/phosphate are not...
Abstract Grape antioxidant dietary fiber (GADF) is a supplement that combines the benefits of both and antioxidants help prevent cancer cardiovascular diseases. The polyphenolic components in GADF probably digestive tract, where they are bioavailable. Mass spectrometry coupled to liquid chromatography powerful tool for analysis complex plant derivatives such as GADF. We use combination MS techniques, namely chromatography/electrospray ionization time‐of‐flight mass (LC/ESI‐TOF‐MS) tandem...
Acidity constants (pKa) of nine flavonoids have been spectrophotometrically determined and the obtained values carefully contrasted with those derived from various experimental approaches given in literature, as well ones predicted by Percepta SPARC programs.To obtain robust pKa values, titrations both pH ways, acid to base vice-versa, performed.The well-known instability most basic media originates some discrepancies between titration ways also coming different previously published.This...
The problem-based learning (PBL) methodology has been applied in a set of chemistry courses at the University Barcelona to determine main factors that are relevant satisfactory application this educational approach different contexts (from undergraduate master subjects). exceptional situation resulting from COVID-19 pandemic also forced us adapt traditional face-to-face modality an online version. Thus, comparative study allowed identify aspects each subject, student background, and used,...
Five in vitro physicochemical systems have been evaluated terms of its ability to emulate the skin permeation neutral compounds: two different PAMPA membranes, classical octanol-water partition coefficient, and biomimetic chromatography systems, one based cerasome electrokinetic another reversed-phase liquid measurements. The coefficients solvation parameter model equation mentioned compared ones process through comparison parameters. Moreover, a method predict whether system is able...
The selectivity of a compilation single, mixed, and modified EKC pseudostationary phases, described in the literature characterized through solvation parameter model, is analyzed. Not only have micellar systems different nature been included but also microemulsions, polymeric, liposomial phases. In order to compare systems, principal component analysis coefficients equation performed. From this analysis, direct information system properties, differences selectivity, as well evidence lack...