Chenglin Chai

ORCID: 0000-0001-7286-4797
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About
Contact & Profiles
Research Areas
  • Plant Molecular Biology Research
  • Soybean genetics and cultivation
  • Plant nutrient uptake and metabolism
  • Legume Nitrogen Fixing Symbiosis
  • Photosynthetic Processes and Mechanisms
  • Plant Gene Expression Analysis
  • GABA and Rice Research
  • Microbial bioremediation and biosurfactants
  • Rice Cultivation and Yield Improvement
  • Genetic Mapping and Diversity in Plants and Animals
  • Plant biochemistry and biosynthesis
  • Antioxidant Activity and Oxidative Stress
  • Plant Virus Research Studies
  • Enzyme-mediated dye degradation
  • RNA and protein synthesis mechanisms
  • Plant Reproductive Biology
  • Advanced biosensing and bioanalysis techniques
  • Plant Disease Resistance and Genetics
  • Agronomic Practices and Intercropping Systems
  • Plant-Microbe Interactions and Immunity
  • Pesticide and Herbicide Environmental Studies
  • Plant tissue culture and regeneration
  • Microbial Community Ecology and Physiology
  • Plant Stress Responses and Tolerance
  • DNA and Nucleic Acid Chemistry

Qingdao Agricultural University
2023

Noble Research Institute
2018-2021

Louisiana State University Agricultural Center
2016-2018

University of Missouri
2015-2016

Norwegian Institute of Bioeconomy Research
2016

Indian Institute of Technology Guwahati
2016

University of Copenhagen
2016

Lanzhou University
2015

University of Maryland, College Park
2013

The Ohio State University
2010-2012

Few regulators of phenylpropanoids have been identified in monocots having potential as biofuel crops. Here we demonstrate the role maize (Zea mays) R2R3-MYB factor ZmMYB31 control phenylpropanoid pathway. We determined its vitro consensus DNA-binding sequence ACC(T)/(A) ACC, and chromatin immunoprecipitation (ChIP) established that it interacts with two lignin gene promoters vivo. To explore a regulator other plants, regulation pathway was further investigated Arabidopsis thaliana....

10.1111/j.1365-313x.2010.04363.x article EN The Plant Journal 2010-09-15

Pre-harvest sprouting (PHS) or vivipary in cereals is an important agronomic trait that results significant economic loss. A considerable number of mutations cause PHS have been identified several species. However, relatively few viviparous mutants rice (Oryza sativa L.) reported. To explore the mechanism rice, we carried out extensive genetic screening and 12 (phs). Based on their phenotypes, these phs were classified into three groups. Here characterize detail one groups, which contains...

10.1111/j.1365-313x.2008.03411.x article EN The Plant Journal 2008-01-16

Abstract Cold stress resulting from chilling and freezing temperatures substantially reduces crop production worldwide. To identify genes critical for cold tolerance in plants, we screened Arabidopsis thaliana mutants deregulated expression of a firefly luciferase reporter gene under the control C-REPEAT BINDING FACTOR2 (CBF2) promoter (CBF2:LUC). A regulator CBF expression1 (rcf1-1) mutant that is hypersensitive to was chosen in-depth characterization. RCF1 encodes cold-inducible DEAD...

10.1105/tpc.112.108340 article EN The Plant Cell 2013-01-01

The maize R2R3-MYB regulator C1 cooperates with the basic helix–loop–helix (bHLH) factor R to activate expression of anthocyanin biosynthetic genes coordinately. As is case for other bHLH factors, harbors several protein–protein interaction domains. Here we show that not classical but rather a briefly extended region forms homodimers bind canonical G-box DNA motifs. This DNA-binding activity abolished if C-terminal ACT (aspartokinase, chorismate, and TyrA) domain licensed homodimerize. Then...

10.1073/pnas.1205513109 article EN Proceedings of the National Academy of Sciences 2012-07-09

Root system architecture is important for water acquisition and nutrient all crops. In soybean breeding programs, wild alleles have been used successfully to enhance yield seed composition traits, but never investigated improve root architecture. Therefore, in this study, high-density single-feature polymorphic markers simple sequence repeats were map quantitative trait loci (QTLs) governing an inter-specific mapping population developed from a cross between Glycine max soja.Wild cultivated...

10.1186/s12864-015-1334-6 article EN cc-by BMC Genomics 2015-02-24

Abstract Background The maize ( Zea mays ) red aleurone1 pr1 encodes a CYP450-dependent flavonoid 3’-hydroxylase (ZmF3’H1) required for the biosynthesis of purple and anthocyanin pigments. We previously showed that Zmf3 ’ h1 is regulated by C1 (Colorless1) R1 (Red1) transcription factors. current study demonstrates that, in addition to its role biosynthesis, gene also participates 3-deoxyflavonoids phlobaphenes accumulate pericarps, cob glumes, silks. Biosynthesis P1 (Pericarp color1)...

10.1186/1471-2229-12-196 article EN cc-by BMC Plant Biology 2012-11-01

The plant phytohormone auxin controls many aspects of growth and development, which largely depends on its uneven distribution in tissues. Transmembrane proteins the PIN family are efflux facilitators. They play a key role polar transport associated with asymmetrical plants. genes have been characterized several species, while comprehensive analysis this gene soybean has not reported yet.In study, twenty-three members were identified genome through homology searches. Analysis chromosome...

10.1186/s12864-015-2149-1 article EN cc-by BMC Genomics 2015-11-16

The plant hormone auxin regulates many aspects of growth and developmental processes. Auxin gradient is formed in as a result polar transportation by three types transporters such OsLAX, OsPIN, OsABCB. We report here the analysis two rice transporter gene families, OsLAX OsABCB, using bioinformatics tools, publicly accessible microarray data, quantitative RT-PCR. There are 5 putative OsLAXs 22 OsABCBs genome, which were mapped on 8 chromosomes. exon-intron structure genes properties deduced...

10.3389/fpls.2016.00593 article EN cc-by Frontiers in Plant Science 2016-05-03

Abstract Analyzing the genome level DNA polymorphisms between weedy and cultivated rice is crucial to elucidate molecular basis of agronomic traits, which in turn can enhance our ability control its utilization for improvement. Here, we presented genome-wide genetic variations a accession PSRR-1 two accessions, Bengal Nona Bokra, belonging japonica indica subspecies, respectively. The total number SNPs InDels PSRR/Bengal was similar that Bokra/Bengal, but three times greater than PSRR/Nona...

10.1038/s41598-018-32513-z article EN cc-by Scientific Reports 2018-09-17

Whole genome sequencing provides the most comprehensive collection of an organism’s genetic information. The availability complete sequences is expected to dramatically deliver a high impact on biology. However, achieve this in area crop improvement, significant efforts are still required functional genomics, including areas gene annotation, cloning, expression profiling, and validation. Here we report our generating first transcription factor (TF) open reading frame (ORF)eome resource...

10.1186/s12864-015-1743-6 article EN cc-by BMC Genomics 2015-08-12

Abstract Although flowering in rice has been extensively investigated, few studies focused on genetic interactions. Flowering evaluation of two recombinant inbred line (RIL) populations involving photo-insensitive cultivars, Bengal and Cypress, a weedy accession, PSRR-1, under natural long-day (LD) conditions, revealed six to ten quantitative trait loci (QTLs) major QTL interaction. In addition the validation several previously cloned genes using an introgression lines (IL) population novel...

10.1038/s41598-018-20324-1 article EN cc-by Scientific Reports 2018-01-26

The phytohormone auxin plays a critical role in regulation of plant growth and development as well responses to abiotic stresses. This is mainly achieved through its uneven distribution plants via polar transport process. Auxin transporters are major players transport. AUXIN RESISTANT 1 ⁄ LIKE AUX1 (AUX⁄LAX) influx carriers belong the amino acid permease family proton-driven function uptake indole-3-acetic (IAA). In this study, genome-wide comprehensive analysis soybean AUX⁄LAX (GmLAX) gene...

10.3389/fpls.2016.00282 article EN cc-by Frontiers in Plant Science 2016-03-09

Aflatoxin, produced by Aspergillus flavus, is hazardous to health of humans and livestock. The lack information about large effect QTL for resistance aflatoxin accumulation a major obstacle employ marker-assisted selection maize improvement. understanding mechanisms the host plant associated genes necessary improving A. flavus infection. A suppression subtraction hybridization (SSH) cDNA library was made using developing kernels Mp715 (resistant inbred) B73 (susceptible 480 randomly selected...

10.3389/fpls.2017.00503 article EN cc-by Frontiers in Plant Science 2017-04-06

Virus-induced gene silencing (VIGS) is a rapid and powerful method to evaluate function, especially for species like hexaploid wheat that have large, redundant genomes are difficult time-consuming transform. The Brome mosaic virus (BMV)-based VIGS vector widely used in monocotyledonous but not wheat. Here we report the establishment of simple effective procedure bread using BMVCP5, most recently improved BMV vector, genes PHYTOENE DESATURASE ( TaPDS ) PHOSPHATE2 TaPHO2 as targets....

10.3389/fpls.2021.685187 article EN cc-by Frontiers in Plant Science 2021-06-18

Abstract Cell‐wall invertase plays an important role in sucrose partitioning between source and sink organs higher plants. To investigate the of cell‐wall invertases for seed development rice ( Oryza sativa L.), cDNAs three putative genes OsCIN1 , OsCIN2 OsCIN3 were isolated. Semi‐quantitative reverse transcription‐polymerase chain reaction analysis revealed different expression patterns various tissues/organs. In developing caryopses, they exhibited similar temporal patterns, expressed...

10.1111/j.1744-7909.2008.00641.x article EN Journal of Integrative Plant Biology 2008-04-01

10.1007/978-1-0716-1661-1_6 article EN Methods in molecular biology 2021-09-20
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