A5016115748- Chronic Lymphocytic Leukemia Research
- Immune Cell Function and Interaction
- CAR-T cell therapy research
- Extracellular vesicles in disease
- Phagocytosis and Immune Regulation
- Cancer Immunotherapy and Biomarkers
- Epigenetics and DNA Methylation
- Galectins and Cancer Biology
- Acute Myeloid Leukemia Research
- MicroRNA in disease regulation
- Immune cells in cancer
- Autophagy in Disease and Therapy
- Cancer-related gene regulation
- Lymphoma Diagnosis and Treatment
- Glycosylation and Glycoproteins Research
- Cancer, Hypoxia, and Metabolism
- Adenosine and Purinergic Signaling
- RNA modifications and cancer
- Systemic Lupus Erythematosus Research
- PI3K/AKT/mTOR signaling in cancer
- Calcium signaling and nucleotide metabolism
- Single-cell and spatial transcriptomics
- Genomics and Chromatin Dynamics
- CRISPR and Genetic Engineering
- Cancer-related Molecular Pathways
Luxembourg Institute of Health
2015-2024
Université de Bourgogne
2008-2015
Centre de recherche Translationnelle en Médecine moléculaire
2013-2015
Laboratoire National de Santé
2011-2014
Inserm
2008-2013
Tumor-derived microvesicles (TD-MVs) are key mediators which shed by cancer cells and can sensitize neighboring in the tumor microenvironment. TD-MVs extracellular vesicles composed of exosomes MVs promote invasion metastasis. Intratumoral hypoxia is an integral component all solid tumors. The relationship between hypoxic tumor-shed NK-mediated cytotoxicity remains unknown. In this paper, we reported that derived from qualitatively differ those normoxic cells. Using multiple models, showed...
In chronic lymphocytic leukemia (CLL), monocytes and macrophages are skewed toward protumorigenic phenotypes, including the release of tumor-supportive cytokines expression immunosuppressive molecules such as programmed cell death 1 ligand (PD-L1). To understand mechanism driving skewing in CLL, we evaluated role tumor cell-derived exosomes cross-talk with monocytes. We carried out RNA sequencing proteome analyses CLL-derived identified noncoding Y hY4 a highly abundant species that is...
Significance The failure in achieving a durable clinical immune response against cancer cells depends on the ability of to establish microenvironment that prevent cytotoxic infiltrate tumors and kill cells. Therefore, key approach successful antitumor is harness strategies allowing reorientation tumor. Herein we reveal inhibiting autophagy induces massive infiltration natural killer into tumor bed, subsequent dramatic decrease volume melanomas. These results highlight role targeting breaking...
Early cancer detection and disease stratification or classification are critical to successful treatment. Accessible, reliable, informative biomarkers can be medically valuable provide some relevant insights into biology. Recent studies have suggested improvements in detecting malignancies by the use of specific extracellular microRNAs (miRNAs) plasma. In chronic lymphocytic leukemia (CLL), an incurable hematologic disorder, sensitive, early, noninvasive diagnosis better would very useful...
Transcription intermediary factor 1γ (TIF1γ) was suggested to play a role in erythropoiesis. However, how TIF1γ regulates the development of different blood cell lineages and whether is involved human hematological malignancies remain be determined. Here we have shown that tumor suppressor mouse chronic myelomonocytic leukemia (CMML). Loss Tif1g HSCs favored expansion granulo-monocytic progenitor compartment. Furthermore, deletion induced age-dependent appearance cell-autonomous...
Abstract Cancer cells fuel their increased need for nucleotide supply by upregulating one-carbon (1C) metabolism, including the enzymes methylenetetrahydrofolate dehydrogenase–cyclohydrolase 1 and 2 (MTHFD1 MTHFD2). TH9619 is a potent inhibitor of dehydrogenase cyclohydrolase activities in both MTHFD1 MTHFD2, selectively kills cancer cells. Here, we reveal that, cells, targets nuclear MTHFD2 but does not inhibit mitochondrial MTHFD2. Hence, overflow formate from mitochondria continues...
Dysregulation of mRNA translation, including preferential translation with complex 5'-UTRs such as the MYC oncogene, is recognized an important mechanism in cancer. In this study, we show that both human and murine chronic lymphocytic leukemia (CLL) cells display a high rate, which can be inhibited by synthetic flavagline FL3, prohibitin (PHB)-binding drug. A multiomics analysis consisting pulsed SILAC, RNA sequencing polysome profiling performed CLL patient samples cell lines treated FL3...
Modeling tumor metabolism in vitro remains challenging. Here, we used galactose as an tool compound to mimic glycolytic limitation. In contrast the established idea that high flux reduces pyruvate kinase isozyme M2 (PKM2) activity support anabolic processes, have discovered limitation also affects PKM2 activity. Surprisingly, despite limited carbon availability and energetic stress, cells induce a near-complete block of divert carbons toward serine metabolism. Simultaneously, TCA cycle is...
The inv(8)(p11q13) chromosomal abnormality, described in acute myeloid leukaemias (AML), fuses the histone acetyl-transferase (HAT) MYST3 (MOZ) gene with another HAT gene, NCOA2 (TIF2). We generated a transgenic zebrafish which MYST3/NCOA2 fusion was expressed under control of spi1 promoter. An AML developed 2 180 MYST3/NCOA2-EGFP-expressing embryos, 14 and 26 months after injection one-cell embryo, respectively. This leukaemia characterised by an extensive invasion kidneys blast cells....
Chronic lymphocytic leukemia (CLL) cells are highly dependent on interactions with the immunosuppressive tumor microenvironment (TME) for survival and proliferation. In search novel treatments, pro-inflammatory cytokines have emerged as candidates to reactivate immune system. Among those, interleukin 27 (IL-27) has recently gained attention, but its effects differ among malignancies. Here, we utilized Eμ-TCL1 EBI3 knock-out mouse models well clinical samples from patients investigate role of...
In this study, we extensively dissected the phenotypic complexity of splenic tumor microenvironment (TME) in chronic lymphocytic leukemia (CLL) by high-dimensional mass cytometry (CyTOF). As a result, identified potential new targets and tested dual immune checkpoint blockade targeting TME pre-clinical mouse models CLL.