A5072501227- MicroRNA in disease regulation
- Melanoma and MAPK Pathways
- Circular RNAs in diseases
- Cytokine Signaling Pathways and Interactions
- Extracellular vesicles in disease
- Virology and Viral Diseases
- Cancer-related molecular mechanisms research
- Respiratory viral infections research
- Cancer Mechanisms and Therapy
- CAR-T cell therapy research
- Single-cell and spatial transcriptomics
- RNA modifications and cancer
- Cell Adhesion Molecules Research
- Advanced biosensing and bioanalysis techniques
- Computational Drug Discovery Methods
- RNA Research and Splicing
- melanin and skin pigmentation
- RNA regulation and disease
- Protein Degradation and Inhibitors
- Vaccine Coverage and Hesitancy
- Immune Cell Function and Interaction
- Spectroscopy Techniques in Biomedical and Chemical Research
- interferon and immune responses
- Anodic Oxide Films and Nanostructures
- Toxin Mechanisms and Immunotoxins
University of Luxembourg
2015-2024
Maricopa County Department of Public Health
2021
TU Dortmund University
2021
Life Sciences Research Foundation
2012
University of the Witwatersrand
1997-1999
Laboratoire National de Santé
1999
Significance The failure in achieving a durable clinical immune response against cancer cells depends on the ability of to establish microenvironment that prevent cytotoxic infiltrate tumors and kill cells. Therefore, key approach successful antitumor is harness strategies allowing reorientation tumor. Herein we reveal inhibiting autophagy induces massive infiltration natural killer into tumor bed, subsequent dramatic decrease volume melanomas. These results highlight role targeting breaking...
Small noncoding microRNAs (miRNA) regulate the expression of target mRNAs by repressing their translation or orchestrating sequence-specific degradation. In this study, we investigated miRNA and gene patterns in melanoma to identify candidate biomarkers for early progressive disease. Because data presently available on are inconsistent thus far, applied several different detection profiling techniques a panel 10 cell lines 20 patient samples representing nevi primary metastatic melanoma....
MicroRNAs (miRNAs) are ubiquitously expressed small non-coding RNAs that, in most cases, negatively regulate gene expression at the post-transcriptional level. miRNAs involved fine-tuning fundamental cellular processes such as proliferation, cell death and cycle control believed to confer robustness biological responses. Here, we investigated simultaneously transcriptional changes of miRNA mRNA levels over time after activation Janus kinase/Signal transducer activator transcription...
Most melanoma patients with BRAFV600E positive tumors respond well to a combination of BRAF kinase and MEK inhibitors. However, some are intrinsically resistant while the majority eventually develop drug resistance treatment. For insufficiently responding inhibitors, there is an ongoing need for new treatment targets. Cellular metabolism such promising target line: mutant has been shown affect metabolism.Time course experiments series western blots were performed in panel BRAFWT/NRASmut...
The type-II-cytokine IFN-γ is a pivotal player in innate immune responses but also assumes functions controlling tumor cell growth by orchestrating cellular against neoplastic cells. role of melanoma not fully understood: it well-known inhibitor cells vitro. On the other hand, may facilitate progression. While interferon-regulated genes encoding proteins have been intensively studied since decades, contribution miRNAs to effects mediated interferons an emerging area research.We recently...
// Christiane Margue 1 , Susanne Reinsbach 1,* Demetra Philippidou Nicolas Beaume Casandra Walters Jochen G. Schneider Dorothée Nashan 1,2 Iris Behrmann Stephanie Kreis Life Sciences Research Unit, University of Luxembourg, Luxembourg 2 Klinikum Dortmund GmbH, Germany * These authors have contributed equally to this work Correspondence to: Kreis, email: Keywords : serum/tissue samples, circulating miRNAs, healthy miRNome, miRNA qPCR arrays, melanoma biomarkers Received January 21, 2015...
The non-coding microRNAs (miRNA) have tissue- and disease-specific expression patterns. They down-regulate target mRNAs, which likely impacts on most fundamental cellular processes. Differential patterns of miRNAs are currently being exploited for identification biomarkers early disease diagnosis, prediction progression melanoma other cancers as promising drug targets, since they can easily be inhibited or replaced in a given context. Before successfully manipulating clinical settings, their...
Drug resistance remains an unsolved clinical issue in oncology. Despite promising initial responses obtained with BRAF and MEK kinase inhibitors, to treatment develops within months virtually all melanoma patients. Microarray analyses were performed inhibitor-sensitive resistant cell lines identify changes the transcriptome that might play a role resistance. siRNA approaches inhibitors used assess involvement of identified Anaplastic Lymphoma Kinase (ALK) drug The capability extracellular...
Background. In developing countries vaccination against measles virus (MV) is generally administered at 9 months of age, although it well-documented that protection most infants by passively acquired maternal MV antibodies waning before immunization given. The purpose this study was to investigate the decay maternally derived in Nigerian as well compare a German and cohort paired mothers newborns regarding placental transfer efficiency MV-specific IgG total antibodies. Methods. were measured...
Reduced levels of intratumoural oxygen are associated with hypoxia-induced pro-oncogenic events such as invasion, metabolic reprogramming, epithelial-mesenchymal transition, metastasis and resistance to therapy, all favouring cancer progression. Small extracellular vesicles (EV) shuttle various cargos (proteins, miRNAs, DNA others). Tumour-derived EVs can be taken up by neighbouring or distant cells in the tumour microenvironment, thus facilitating intercellular communication. The quantity...
Melanoma has the highest propensity of all cancers to metastasize brain with a large percentage late-stage patients developing metastases in central nervous system (CNS). It is well known that metastasis establishment, cell survival, and progression are affected by tumour-host interactions where changes host cellular compartments likely play an important role. In this context, miRNAs transferred tumour derived extracellular vesicles (EVs) have previously been shown create favourable...
The cytoskeletal protein talin, which provides a direct link between integrins and actin filaments, has been shown to contain two distinct binding sites for integrin β subunits. Here, we report the precise delimitation first functional analysis of talin rod domain integrin-binding site. Partially overlapping cDNAs covering entire human gene were transiently expressed as DsRed fusion proteins in Chinese hamster ovary cells expressing αIIbβ3, linked green fluorescent (GFP). Two-color...
Signal transducers and activators of transcriptions (STAT) are key mediators cytokine signaling. Moreover, these transcription factors play a crucial role in oncogenic signaling where inappropriate sustained activation STATs, especially STAT3, is trait many different cancers their derived cell lines. Constitutively active STAT3 has been reported to prevent programmed death enhance proliferation, whereas the disruption can inhibit tumor growth. The physiologic by cytokines well established;...
MicroRNAs are major players in post-transcriptional gene regulation. Even small changes miRNA levels may have profound consequences for the expression of target genes. Hence, miRNAs themselves need to be tightly, albeit dynamically, regulated. Here, we investigated dynamic behavior over a wide time range following stimulation melanoma cells with interferon-γ (IFN-γ), which activates transcription factor STAT1. By applying several bioinformatic and statistical software tools visualization...
Interleukin-6 (IL-6)-type cytokines share the common receptor glycoprotein 130 (gp130), which activates a signaling cascade involving Janus kinases (JAKs) and signal transducer activator of transcription (STAT) factors. IL-6 and/or its pathway is often deregulated in diseases, such as chronic liver diseases cancer. Thus, identification compounds inhibiting this interest for future targeted therapies. We established novel cellular screening systems based on STAT-responsive reporter gene...