A5010203392- Hepatitis C virus research
- Hepatitis B Virus Studies
- Monoclonal and Polyclonal Antibodies Research
- Liver Disease Diagnosis and Treatment
- SARS-CoV-2 and COVID-19 Research
- Animal Virus Infections Studies
- Animal Disease Management and Epidemiology
- HIV Research and Treatment
- T-cell and Retrovirus Studies
- Viral Infectious Diseases and Gene Expression in Insects
- Plant Virus Research Studies
- Vector-Borne Animal Diseases
- Influenza Virus Research Studies
- RNA and protein synthesis mechanisms
- Viral Infections and Outbreaks Research
Imperial College London
2023-2024
University College London
2018-2023
Institute of Infection and Immunity
2018-2022
The Royal Free Hospital
2020
Centre for Immunity, Infection and Evolution
2019
The recent emergence of SARS-CoV-2 variants with increased transmission, pathogenesis and immune resistance has jeopardised the global response to COVID-19 pandemic. Determining fundamental biology viral understanding their evolutionary trajectories will guide current mitigation measures, future genetic surveillance vaccination strategies. Here we examine virus entry by B.1.1.7 lineage, commonly referred as UK/Kent variant. Pseudovirus infection model cell lines demonstrate that is enhanced...
The emergence of SARS-CoV-2 variants has exacerbated the COVID-19 global health crisis. Thus far, all carry mutations in spike glycoprotein, which is a critical determinant viral transmission being responsible for attachment, receptor engagement and membrane fusion, an important target immunity. Variants frequently bear truncations flexible loops N-terminal domain (NTD) spike; functional importance these modifications remained poorly characterised. We demonstrate that NTD deletions are...
Great strides have been made in understanding and treating hepatitis C virus (HCV) thanks to the development of various experimental systems including cell-culture-proficient HCV, HCV pseudoparticle system soluble envelope glycoproteins. The (HCVpp) is a platform used extensively studies cell entry, screening novel entry inhibitors, assessing phenotypes clinically observed E1 E2 glycoproteins and, most pertinently, characterizing neutralizing antibody breadth induced upon vaccination natural...
The mechanism by which hepatitis C virus (HCV) gains entry into cells is a complex one, involving broad range of host proteins. Entry critical phase the viral lifecycle, and potential target for therapeutic or vaccine-mediated intervention. However, mechanics HCV remain poorly understood. Here we describe novel computational model entry, encompassing relationship between key receptors CD81 SR-B1. We conduct experiments to thoroughly quantify influence an increase decrease in receptor...
With an estimated 10 million people infected, the deltaretrovirus human T-cell lymphotropic virus type 1 (HTLV-1) is second most prevalent pathogenic retrovirus in humans after HIV-1. Like HIV-1, HTLV-1 overwhelmingly persists a host via reservoir of latently infected CD4+ T cells. Although patients are asymptomatic, HTLV-1-associated pathologies often debilitating and include adult leukaemia/lymphoma (ATLL), which presents mature adulthood associated with poor prognosis short overall...
E1 and E2 (E1E2), the fusion proteins of Hepatitis C Virus (HCV), are unlike that any other virus yet described, detailed molecular mechanisms HCV entry/fusion remain unknown. Hypervariable region-1 (HVR-1) is a putative intrinsically disordered protein tail. Here, we demonstrate HVR-1 has an autoinhibitory function suppresses activity E1E2 on free virions; this dependent its conformational entropy. Thus, akin to safety catch prevents premature triggering activity. Crucially, mechanism...
Great strides have been made in understanding and treating Hepatitis C virus (HCV) thanks part to the development of full-length cell-culture system, pseudoparticle system soluble envelope glycoproteins. The HCV (HCVpp) is a platform used extensively studies cell entry, screening novel entry inhibitors, assessing phenotypes clinically observed E1 E2 glycoproteins and, most pertinently, characterising neutralizing antibody breadth induced upon vaccination natural infection patients....
With an estimated 10 million people infected, the deltaretrovirus human T-cell lymphotropic virus type 1 (HTLV-1) is second most prevalent pathogenic retrovirus in humans after HIV-1. Like HIV-1, HTLV-1 overwhelmingly persists a host via reservoir of latently infected CD4+ T cells. Although patients are asymptomatic, HTLV-1-associated pathologies often debilitating and include childhood infective dermatitis adult leukaemia/lymphoma (ATLL), which presents mature adulthood associated with poor...
Due to increased and broadened screening efforts, the last decade has seen a rapid expansion in number of viral species classified into
E1 and E2 (E1E2), the entry proteins of Hepatitis C Virus (HCV), are unlike that any other virus yet described, detailed molecular mechanisms HCV entry/fusion remain unknown. Hypervariable region-1 (HVR-1) is a putative intrinsically disordered protein tail. Here, we demonstrate HVR-1 has an autoinhibitory function suppresses activity E1E2 on free virions; this dependent its conformational entropy. Crucially, to allow entry, mechanism turned off by host receptor interactions at cell surface....
Abstract The mechanism by which hepatitis C virus (HCV) gains entry into cells is a complex one, involving broad range of host proteins. Entry critical phase the viral lifecycle, and potential target for therapeutic or vaccine-mediated intervention. However, mechanics HCV remain poorly understood. Here we describe novel computational model entry, encompassing relationship between key receptors CD81 SR-B1. We conduct experiments to thoroughly quantify influence an increase decrease in...
Abstract Due to increased and broadened screening efforts, the last decade has seen a rapid expansion in number of viral species classified into Hepacivirus genus. Conserved genetic features hepaciviruses suggest they have undergone specific adaptation evolved hijack similar host proteins for efficient propagation liver. Here, we developed pseudotyped viruses elucidate entry factors GB virus-B (GBV-B), first hepacivirus described an animal, closely related hepatitis C virus (HCV). GBV-B...