A5070778208- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Cerebrovascular and Carotid Artery Diseases
- Intracranial Aneurysms: Treatment and Complications
- Immunotherapy and Immune Responses
- Traumatic Brain Injury and Neurovascular Disturbances
- Cancer Immunotherapy and Biomarkers
- Single-cell and spatial transcriptomics
- Acute Ischemic Stroke Management
- Peptidase Inhibition and Analysis
- Adrenal Hormones and Disorders
- Ion channel regulation and function
- Melanoma and MAPK Pathways
- Cardiovascular Health and Disease Prevention
- Circadian rhythm and melatonin
- Advanced MRI Techniques and Applications
- Chemokine receptors and signaling
- Vascular Malformations Diagnosis and Treatment
- Neurological Complications and Syndromes
- Reproductive Biology and Fertility
- Silk-based biomaterials and applications
- Peripheral Artery Disease Management
- Moyamoya disease diagnosis and treatment
- Invertebrate Immune Response Mechanisms
- Pituitary Gland Disorders and Treatments
CSIRO Manufacturing
2025
The University of Texas at Austin
2019-2023
MIND Research Institute
2016
Weill Cornell Medicine
2016
Cornell University
2016
University of Washington
1996-2003
Northwestern University
2001-2002
Swedish Medical Center
2002
Neurological Surgery
1996-2001
Universität Hamburg
1998
Immune checkpoint inhibitors (ICI) targeting CTLA4 or PD-1/PD-L1 have transformed cancer therapy but are associated with immune-related adverse events, including myocarditis. Here, we report a robust preclinical mouse model of ICI-associated myocarditis in which monoallelic loss Ctla4 the context complete genetic absence Pdcd1 leads to premature death approximately half mice. Premature results from myocardial infiltration by T cells and macrophages severe ECG abnormalities, closely...
The type IIA rat brain sodium channel is composed of three subunits: a large pore-forming α subunit and two smaller auxiliary subunits, β1 β2. β subunits are single membrane-spanning glycoproteins with one Ig-like motif in their extracellular domains. Ig the β2 has close structural similarity to six motifs domain cell adhesion molecule contactin (also called F3 or F11), which binds matrix molecules tenascin-C tenascin-R. We investigated binding purified tenascin-R vitro . Incubation channels...
A key feature of the inflammatory response after cerebral ischemia is brain infiltration blood monocytes. There are two main monocyte subsets in mouse blood: CCR2+Ly6Chi "inflammatory" monocytes involved acute inflammation, and CX3CR1+Ly6Clo "patrolling" monocytes, which may play a role repair processes. We hypothesized that recruited early phase transdifferentiate into "repair" macrophages brain. CX3CR1GFP/+CCR2RFP/+ bone marrow (BM) chimeric mice underwent transient middle artery occlusion...
Background and Purpose A subject with dissection of the internal carotid artery (ICA) may present a variety symptoms, from headache to stroke. Thus far, it has not been possible identify subset patients at risk for cerebral ischemia. Because majority these ischemic events are secondary embolic phenomena, we used transcranial Doppler (TCD) evaluation emboli monitoring study 17 consecutive ICA treated Harborview Medical Center, Seattle, Wash, during 2-year period 1992 until 1994. Methods Ten...
Abstract Both medullary thymic epithelial cells (mTEC) and dendritic (DC) present tissue-restricted antigens (TRA) to thymocytes induce central tolerance, but the relative contributions of these antigen-presenting cell (APC) subsets remain unresolved. Here we developed a two-photon microscopy approach observe interacting with intact APCs presenting TRAs. We find that mTECs DCs cooperate extensively their regulated by cellular form TRA class major histocompatibility complex (MHC) on which...
Age-related thymus involution results in decreased T-cell production, contributing to increased susceptibility pathogens and reduced vaccine responsiveness. Elucidating mechanisms underlying will inform strategies restore thymopoiesis with age. The is colonized by circulating bone marrow (BM)-derived seeding progenitors (TSPs) that differentiate into early (ETPs). We find ETP cellularity declines as 3 months (3MO) of age mice. This initial reduction could reflect changes thymic stromal...
SUMMARY T cells in the perinatal thymus have distinct phenotypes and functions that may be instructed by age-specific features of microenvironment. We evaluated molecular cellular profiles thymic stromal cells, including epithelial (TECs), mesenchyme, endothelium, hematopoietic antigen presenting (hAPCs), from birth through one-month age mice. Single-cell transcriptional profiling, flow cytometry, immunohistochemistry revealed coordinated changes accompanied altered thymocyte differentiation...
Abstract One of the earliest hallmarks immune aging is thymus involution, which not only reduces number newly generated and exported T cells, but also alters composition organization microenvironment. Thymic T‐cell export continues into adulthood, yet impact involution on quality clones well established. Notably, proportion medullary thymic epithelial cells (mTECs) expression tissue‐restricted antigens (TRAs) decline with age, suggesting involuting may promote efficient central tolerance....
Central tolerance ensures autoreactive T cells are eliminated or diverted to the regulatory cell lineage, thus preventing autoimmunity. To undergo central tolerance, thymocytes must enter medulla test their T-cell receptors (TCRs) for autoreactivity against diverse self-antigens displayed by antigen-presenting (APCs). While CCR7 is known promote thymocyte medullary entry and negative selection, our previous studies implicate CCR4 in these processes, raising question of whether play distinct...
DUPLEX ULTRASONOGRAPHY IS frequently used for noninvasive screening extracranial occlusive disease. In a multicenter, prospective, randomized trial carotid endarterectomy in symptomatic patients, the duplex ultrasound examination was compared with conventional angiographic findings internal artery (ICA) stenosis 178 patients. Angiograms were interpreted by radiologists both at local medical centers and central site; determination of ICA calculated as percent diameter point maximal narrowing...
✓ Accurate placement of parietooccipital ventricular catheters can be difficult and frustrating. To minimize the morbidity procedure lengthen duration shunt function, catheter tip should lie in ipsilateral frontal horn. The authors describe a posterior guide (PVG) for that operates by mechanically coupling burr hole to anterior target point. In series 38 patients who underwent ventriculoperitoneal shunting with assistance guide, postoperative computerized tomography (CT) scanning revealed 35...
Suprasellar masses in children include lesions such as craniopharyngiomas and germ cell neoplasms. The difficult location of these their proximity to important neural vascular structures pose challenges resection. We operated on 14 patients using a bifrontal craniotomy with removal both orbital rims provide access suprasellar masses, even those significant extension into the third ventricle. A complete resection was possible 8/14 8/10 craniopharyngiomas. In 13 patients, optic nerves were...
Internal carotid pseudoaneurysm and cerebral infarction from shotgun pellet penetration embolization.J K Song, J Srinivasan, D S Gordon, W Newell A B BaxterAudio Available | Share
SUMMARY Dendritic cells (DCs) are essential for establishing central tolerance in the thymus; however, mechanisms supporting their homeostasis and activation remain unresolved. Through single-cell transcriptomics functional assays, we identify seven thymic conventional DC (cDC) subsets discriminate abilities to present self-antigens induce regulatory T (Tregs). Mice blocked at different stages of T-cell development reveal that CD4 + single-positive (SP) CD8SP thymocytes differentially...
Cerebral angioplasty has been reported to be useful for the treatment of symptomatic cerebral vasospasm following subarachnoid hemorrhage [3]. Recent data indicates that may effective in preventing development [7]. However, long-term effects on human circulation are not known. In coronary circulation, complications atherosclerotic disease include restenosis 30% vessels, occlusion vessel, transient loss vascular reactivity, and endothelial dysfunction [4]. induces injury initiates intimai...
Summary One of the earliest hallmarks immune aging is thymus involution, which not only reduces number newly generated and exported T cells, but also alters composition organization thymic microenvironment. Thymic T-cell export continues into adulthood, yet impact involution on quality clones well established. Notably, proportion medullary epithelial cells (mTECs) expression tissue restricted antigens (TRAs) decline with age, suggesting involuting may promote efficient central tolerance....