A5045970179- Cell death mechanisms and regulation
- interferon and immune responses
- Immune Response and Inflammation
- RNA Research and Splicing
- Medical Imaging Techniques and Applications
- Bacillus and Francisella bacterial research
- Antimicrobial Peptides and Activities
- Cancer-related Molecular Pathways
- Sperm and Testicular Function
- MicroRNA in disease regulation
- RNA modifications and cancer
- Nuclear Structure and Function
- Radiomics and Machine Learning in Medical Imaging
- Neuroendocrine Tumor Research Advances
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- NF-κB Signaling Pathways
- Eosinophilic Disorders and Syndromes
- Neurobiology and Insect Physiology Research
- Circular RNAs in diseases
- Biochemical and Structural Characterization
- Medical Imaging and Pathology Studies
- Cardiovascular Effects of Exercise
- Asthma and respiratory diseases
- Radiation Dose and Imaging
- Advanced Radiotherapy Techniques
Hanyang University
2000-2024
University of Cologne
2014-2024
Cologne Excellence Cluster on Cellular Stress Responses in Aging Associated Diseases
2014-2024
Ansan University
2023
Chosun University
2017-2019
Harvard University
2006-2015
Brigham and Women's Hospital
2015
Sidney Kimmel Comprehensive Cancer Center
2015
Johns Hopkins University
2015
SUNY Old Westbury
2012
Abstract The serine/threonine kinase RIPK1 is recruited to TNFR1 mediate proinflammatory signaling and regulate TNF-induced cell death. A deficiency results in perinatal lethality, impaired NFκB MAPK signaling, sensitivity apoptosis. Chemical inhibitor vitro–reconstitution studies suggested that displays distinct activity–dependent –independent functions. To determine the contribution of inflammation vivo, we generated knock-in mice endogenously expressing catalytically inactive D138N....
TNF is an inflammatory cytokine that upon binding to its receptor, TNFR1, can drive production, cell survival, or death. TNFR1 stimulation causes activation of NF-κB, p38α, and downstream effector kinase MK2, thereby promoting transcription, mRNA stabilization, translation target genes. Here we show TNF-induced MK2 results in global RIPK1 phosphorylation. directly phosphorylates at residue S321, which inhibits ability bind FADD/caspase-8 induce RIPK1-kinase-dependent apoptosis necroptosis....
RIPK1 is a multi-functional kinase that regulates cell death and inflammation has been implicated in the pathogenesis of inflammatory diseases. acts kinase-dependent kinase-independent manner to promote or suppress apoptosis necroptosis, but underlying mechanisms remain poorly understood. Here, we show mutation (R588E) disrupting domain (DD) caused perinatal lethality induced by ZBP1-mediated necroptosis. Additionally, these mice developed postnatal pathology, which was mediated...
Anthrax caused by Bacillus anthracis represents a major bioterroristic threat. B. produces lethal toxin (LeTx), combination of factor (LF) and protective antigen that plays role in anthrax pathogenesis. We demonstrate human neutrophil α-defensins are potent inhibitors LF. The inhibition LF protein (HNP-1) was noncompetitive. HNP-1 inhibited cleavage mitogen-activated kinase restored impaired signaling LeTx-treated macrophages. rescued murine macrophages from -induced cytotoxicity, vivo...
Abstract The nuclear factor kappa B ( NF ‐κB) signalling pathway exhibits both tumour‐promoting and tumour‐suppressing functions in different tissues models of carcinogenesis. In particular epidermal keratinocytes, ‐κB was reported to exert primarily growth inhibitory functions. Here, we show that mice with keratinocyte‐restricted p65/RelA deficiency were resistant 7, 12‐dimethylbenz(a)anthracene DMBA )‐/12‐O‐tetra decanoylphorbol‐13 acetate TPA )‐induced skin p65 sensitized keratinocytes...
Bacillus anthracis, the etiologic agent of anthrax, avoids immune surveillance and commandeers host macrophages as a vehicle for lymphatic spreading. Here, we show that B. anthracis edema toxin (ET), via its adenylyl cyclase activity, dramatically increases motility infected expression vascular endothelial growth factor. The transcription factor CREB syndecan-1 gene, target, play crucial roles in ET-induced macrophage migration. These molecular cellular responses occur engaged...
Recent evidence demonstrates that activated eosinophils undergo a distinct form of lytic cell death, accompanied by formation DNA-based eosinophil extracellular trap (EET) and degranulation, enhancing inflammatory immune responses in asthmatic airways. We previously showed human blood degranulation response to lysophosphatidylserine (LysoPS), an lipid mediator, strongly express P2Y10, LysoPS receptor.We evaluated EET, death various concentrations LysoPS. also compared responsiveness between...
Various bacterial pathogens secrete toxins, which are not only responsible for fatal pathogenesis of disease, but also facilitate evasion host defences. One the best-known toxin groups is mono-ADP-ribosyltransferase family. In present study, we demonstrate that human neutrophil α-defensins potent inhibitors enzymes, particularly against DT (diphtheria toxin) and ETA (Pseudomonas exotoxin A). HNP1 (human protein 1) inhibited DT- or ETA-mediated ADP-ribosylation eEF2 (eukaryotic elongation...
MicroRNAs (miRNAs) are closely associated with a number of cellular processes, including cell development, differentiation, proliferation, carcinogenesis, and apoptosis. The aim the present study was to elucidate molecular mechanisms underlying tumor suppressor activity miRNA-203 (miR-203) in YD-38 human oral cancer cells.Polymerase chain reaction analysis, MTT assay, DNA fragmentation fluorescence-activated cell-sorting gene array, immunoblotting, luciferase assay were carried out...
In a retrospective study of 14 cases duodenal neoplasms evaluated by computed tomography (CT), there were four primary adenocarcinomas the duodenum, one lymphoma, five metastatic carcinomas, two lipomas, villous adenoma, and leiomyoma. The CT findings diagnostic in patients with lipomas. 11 cases, origin mass was clearly identifiable duodenum. Thickening bowel wall, tumor necrosis, ulceration, intraluminal defects common. relationship masses to adjacent structures shown on scans....
The purpose of this study was to elucidate the molecular mechanism underlying regulation semaphorin-6A (SEMA6A) involving microRNA-203 (miR-203) as a tumor suppressor in YD-38 human oral cancer cells.miRNA arrays, polymerase chain reaction analyses, MTT assays, immunoblotting, and luciferase assays were carried out cells.MiRNA microarray results showed that expression miR-203 significantly down-regulated cells compared normal keratinocytes. viability reduced by time- dose-dependent manners....
Adenosine has been identified to occur abundantly intra-and extracellularly, and exert diverse biological functions, including the suppression of cell proliferation induction apoptosis. reported induce apoptosis in several cancer lines; however, best our knowledge, effect adenosine on head neck cells not investigated. Therefore, purpose present study was evaluate whether exerts any anticancer via human pharyngeal squamous carcinoma FaDu cells. An MTT assay demonstrated that adenosine‑treated...
Platelets play a modulatory role in inflammatory response by secreting vast array of granules and disintegrating into membrane-bound microparticles upon activation. The interplay between eosinophils platelets is postulated to be implicated the pathology allergic airway inflammation. In this study, we investigated whether activated can induce eosinophil extracellular trap (EET) formation, cellular process which release net-like DNA fibers. were stimulated with calcium ionophore, A23187,...
Other SectionsABSTRACTINTRODUCTIONADAR1ZBP1FUTURE PERSPECTIVESACKNOWLEDGEMENTSCONFLICTS OF INTERESTREFERENCES
Eukaryotic gene expression requires multiple cellular events, including transcription and RNA processing transport. Sus1, a common subunit in both the Spt-Ada-Gcn5 acetyltransferase (SAGA) export complex-2 (TREX-2) complexes, is key factor coupling activation to mRNA nuclear export. Here, we report that SAGA DUB module TREX-2 distinctly regulate yeast replicative lifespan Sir2-dependent -independent manner, respectively. The growth impaired by SUS1 loss depend on but not module. Notably, an...
Dorsal defect of the patella (DDP) is a benign lesion with characteristic radiologic features. We describe MR appearance typical DDP and healed as seen in two patients. The classic CT also described.