A5077533501- Ubiquitin and proteasome pathways
- Genomics and Chromatin Dynamics
- Epigenetics and DNA Methylation
- RNA Research and Splicing
- Fungal and yeast genetics research
- Cancer-related gene regulation
- RNA modifications and cancer
- Genetics, Aging, and Longevity in Model Organisms
- Histone Deacetylase Inhibitors Research
- Nuclear Structure and Function
- Genetic and Kidney Cyst Diseases
- Redox biology and oxidative stress
- DNA Repair Mechanisms
- Polyamine Metabolism and Applications
- Telomeres, Telomerase, and Senescence
- Mitochondrial Function and Pathology
- Microtubule and mitosis dynamics
- Glycosylation and Glycoproteins Research
- Genomics and Rare Diseases
- GDF15 and Related Biomarkers
- Lysosomal Storage Disorders Research
- Per- and polyfluoroalkyl substances research
- Birth, Development, and Health
- Genomic variations and chromosomal abnormalities
- Connexins and lens biology
Kyungpook National University
2020-2025
Yale University
2016-2024
Osong Medical Innovation Foundation
2024
Whitney Museum of American Art
2020
Hanyang University
2013-2019
Abstract Histones are substrates of the SUMO (small ubiquitin-like modifier) conjugation pathway. Several reports suggest histone sumoylation affects transcription negatively, but paradoxically, our genome-wide analysis shows modification concentrated at many active genes. We find that trans-tail regulation histone-H2B ubiquitylation and H3K4 di-methylation potentiates subsequent sumoylation. Consistent with known control Set3 deacetylase complex (HDAC) by di-methylation, directly recruits...
Abstract The relationship between aging and RNA biogenesis trafficking is attracting growing interest, yet the precise mechanisms are unknown. THO complex crucial for mRNA cotranscriptional maturation export. Herein, we report that closely linked to regulation of lifespan. Deficiencies in Hpr1 Tho2, components complex, reduced replicative lifespan (RLS) a novel Sir2‐independent RLS control pathway. Although transcript sequestration hpr1Δ or tho2Δ mutants was countered by exosome component...
Pterygium, abnormal growths of conjunctival tissue onto the cornea, are common ocular surface conditions with a high risk recurrence after surgery and potential ophthalmic complications. The exact cause pterygium remains unclear, triggers still unknown. This study aims to investigate relationship between epigenetics uncover identify biomarkers for its diagnosis. We performed ChIP-seq assay compare genome-wide histone modification levels normal conjunctiva stage 3 samples. In this study, we...
Primary cilia are dynamic sensory organelles orchestrating key signaling pathways, and disruption of primary ciliogenesis is implicated in a spectrum genetic disorders. The peroxisomal bifunctional enzyme HSD17B4 pivotal for β-oxidation acetyl-CoA synthesis, its deficiency profoundly impairs metabolism. While patients with exhibit ciliopathy-like symptoms due to dysfunctional cilia, the molecular connection between ciliopathy remains poorly understood. Here, we demonstrate that alters...
Post-translational protein modification by the small ubiquitin-related modifier (SUMO) regulates numerous cellular pathways, including transcription, cell division, and genome maintenance. The SUMO protease Ulp2 modulates many of these SUMO-dependent processes in budding yeast. From whole-genome RNA sequencing (RNA-seq), we unexpectedly discovered that cells lacking display a twofold increase transcript levels across two particular chromosomes: chromosome I (ChrI) ChrXII. This is due to...
In response to acute loss of the Ulp2 SUMO-specific protease, yeast become disomic for chromosome I (ChrI) and ChrXII. Here we report that ChrI disomy, which creates an adaptive advantage in part by increasing dosage Ccr4 deadenylase, was eliminated extended passaging. Loss aneuploidy is often accompanied mutations essential SUMO-ligating enzymes, reduced polySUMO-conjugate accumulation. The mRNA levels almost all ribosomal proteins increase transiently upon initial Ulp2, but elevated limit...
Primary cilia help to maintain cellular homeostasis by sensing conditions in the extracellular environment, including growth factors, nutrients, and hormones that are involved various signaling pathways. Recently, we have shown enhanced primary ciliogenesis dopamine neurons promotes neuronal survival a Parkinson's disease model. Moreover, performed fecal metabolite screening order identify several candidates for improving ciliogenesis, L-carnitine acetyl-L-carnitine. However, role of...
The actin-based cytoskeleton is considered a fundamental driving force for cell differentiation and development. Destrin (Dstn), member of the actin-depolymerizing factor family, regulates actin dynamics by treadmilling filaments increasing globular pools. However, specific developmental roles dstn have yet to be fully elucidated. Here, we investigated physiological functions during early embryonic development using Xenopus laevis as an experimental model organism. expressed in anterior...
How evolution can be facilitated by epigenetic mechanisms has received refreshed attention recently. To explore the role inheritance plays in evolution, we subject isogenic wild-type yeast cells expressing PGAL1-YFP (yellow fluorescent protein) to selection daily sorting based on reporter expression. We observe expression-level reductions multiple replicates sorted for lowest expression that persist several days, even after lifting pressure. Reduced is due factors galactose (GAL) network...
Glutathione peroxidase 3 (GPx3) belongs to the glutathione family of selenoproteins and is a key antioxidant enzyme in multicellular organisms against oxidative damage. Downregulation GPx3 affects tumor progression metastasis associated with liver heart disease. However, physiological significance vertebrate embryonic development remains poorly understood. The current study aimed investigate functional roles gpx3 during embryogenesis. To this end, we determined gpx3’s spatiotemporal...
Primary cilia are crucial for cellular balance, serving as sensors external conditions. Nephronophthisis and related ciliopathies, which hereditary degenerative, stem from genetic mutations in cilia-related genes. However, the precise mechanisms of these conditions still not fully understood. Our research demonstrates that downregulating PDIA6, leading to removal, makes cells more sensitive ferroptotic death caused by endoplasmic reticulum (ER) stress. The reduction PDIA6 intensifies ER...
Primary cilia mediate the interactions between cells and external stresses. Thus, dysregulation of primary is implicated in various ciliopathies, e.g., degeneration retina caused by photoreceptor cilium. Particulate matter (PM) can cause epithelium injury endothelial dysfunction increasing oxidative stress inflammatory responses. Previously, we showed that PM disrupts formation retinal pigment (RPE) cells. In present study, identified 2-isopropylmalic acid (2-IPMA) as a novel inducer...