A5051627431- Pregnancy and preeclampsia studies
- Birth, Development, and Health
- Reproductive System and Pregnancy
- Connective Tissue Growth Factor Research
- Neonatal Respiratory Health Research
- Connexins and lens biology
- Pregnancy and Medication Impact
- Biomarkers in Disease Mechanisms
- COVID-19 Impact on Reproduction
- Gestational Diabetes Research and Management
- Pregnancy-related medical research
- Prenatal Screening and Diagnostics
- Maternal and fetal healthcare
- Congenital Diaphragmatic Hernia Studies
- Ovarian function and disorders
- Preterm Birth and Chorioamnionitis
- Cancer Risks and Factors
- Epigenetics and DNA Methylation
- Immune Response and Inflammation
- COVID-19 and healthcare impacts
- Reproductive Biology and Fertility
- Heme Oxygenase-1 and Carbon Monoxide
- Hemoglobinopathies and Related Disorders
- Renal and related cancers
- Cell Adhesion Molecules Research
University of Duisburg-Essen
2015-2024
Essen University Hospital
2015-2024
Klinik für Frauenheilkunde
2016-2024
Universitätsklinik für Frauenheilkunde und Geburtshilfe
2020
Universitäts Frauenklinik
2017
Institute of Molecular Biology
2008-2015
Carl von Ossietzky Universität Oldenburg
2001
The gap junction protein connexin43 (Cx43) is thought to be involved in growth control several tissues. Using the doxycycline inducible tet-on system, we generated human malignant trophoblast Jeg3 cells transfected with either Cx40, Cx43, or C-terminal truncated Cx43 (trCx43). but not Cx40 trCx43, displayed a reduced cell of vitro and tumor nude mice, suggesting role C terminus regulation. gene array analysis, regulator NOV (CCN3), member CCN family, was found up-regulated only...
Background: The risk of preterm birth (PTB) and stillbirth increases after a SARS-CoV-2 infection during gestation. We aimed to estimate the depending on gestational age at (early <28 + 0 late ≥28 weeks gestation, WoG), virus variants, severity infection, vaccination. Methods: PTB was divided into early (<32 0) (32 0–36 6 WoG). prospective register COVID-19 Related Obstetrics Neonatal Outcome Study (CRONOS) included 8032 pregnant women with confirmed from 3 April 2020 31 December 2022,...
Placenta accreta spectrum (PAS) is one of the major causes maternal morbidity and mortality worldwide with increasing incidence. PAS refers to a group pathological conditions ranging from abnormal attachment placenta uterus wall its perforation and, in extreme cases, invasion into surrounding organs. Among them, characterized by direct adhesion villi myometrium without remains most common diagnosis PAS. Here, we identify potential regulatory miRNA target networks contributing development....
The pregnancy disorder pre-eclampsia (PE) is thought to be caused in part by shallow invasion of the extravillous trophoblast (EVT) leading uteroplacental insufficiency and hypoxia. Here, we focused on expressions cysteine-rich 61 (CYR61, CCN1) nephroblastoma overexpressed (NOV, CCN3), members CCN family angiogenic regulators, human placenta during normal compared with pre-eclamptic HELLP placentae using quantitative RT–PCR, western blotting immunocytochemistry. During pregnancy, both...
Connexin expression and gap junctional intercellular communication (GJIC) mediated by connexin 43 (Cx43)/gap junction A1 (GJA1) are required for cytotrophoblast fusion into the syncytium, outer functional layer of human placenta. Cx43 also impacts intracellular signaling through protein-protein interactions. The transcription factor GCM1 its downstream target ERVW-1/SYNCYTIN-1 key players in trophoblast exert their actions ERVW-1 receptor SLC1A5/ASCT-2/RDR/ATB(0). To investigate molecular...
In search of potential early biomarkers for timely prediction gestational diabetes mellitus (GDM), we focused on afamin, a vitamin E–binding protein in human plasma.. Afamin plays role anti-apoptotic cellular processes related to oxidative stress and is associated with insulin resistance other features metabolic syndrome. During uncomplicated pregnancy its serum concentrations increase linearly. The aim this study was investigate the suitability afamin as marker predicting GDM....
The RUPP procedure is a well-established model for studying the link between placental ischemia and hypertension in pregnant rat has been described detail previously.( 1) First timed Sprague-Dawley rats purchased from Harlan Sprague Dawley Inc. (Indianapolis, IN) were used this study.Twenty-eight randomly assigned to either NP, NP rAgt-targeting siRNA, or siRNA group.On day 12 of gestation (GD), was subcutaneously injected into (10 mg/kg).On gestational 14, under isoflurane
One driving factor for developing preeclampsia—a pregnancy disorder, often associated with poor spiral artery (SpA)-remodeling and fetal growth restriction—is the anti-angiogenic sFLT1 (soluble fms-like tyrosine kinase-1), which is found to be highly upregulated in preeclampsia patients. The sFLT1-mediated endothelial dysfunction a common theory manifestation of maternal symptoms. However, influence on SpA-remodeling link between placental symptoms less understood. To dissect hsFLT1 (human...
It is known that a hypoxic environment critical for trophoblast migration and invasion fundamental appropriate placental perfusion. Because cysteine-rich 61 (CYR61, CCN1) nephroblastoma overexpressed (NOV, CCN3) are expressed in the extravillous expression levels deregulated preeclampsia, we investigated their regulation properties first-trimester explants JEG3 choriocarcinoma cells upon physiological low oxygen tension of 1–3%. In explants, both proteins were increased hypoxia. revealed...
Autophagy is a self-degradative process that involves turnover and recycling of cytoplasmic components in healthy diseased tissue. has been shown to be protective at the early stages programmed cell death but it can also promote apoptosis under certain conditions. Earlier we demonstrated oxygen contributes pathogenesis neonatal brain damage, which ameliorated by intervention with recombinant human erythropoietin (rhEpo). Extrinsic- intrinsic apoptotic pathways are involved induced...
Since it is known that placental overexpression of the human anti-angiogenic molecule sFlt-1, main candidate in progression preeclampsia, lead to intrauterine growth restriction (IUGR) mice by lentiviral transduction mouse blastocysts, we hypothesize sFlt-1 influence morphology and physiology resulting fetal IUGR. We therefore examined effect on at embryonic day 18.5 with histologic morphometric analyses, transcript immunoblotting, methylation studies. Interestingly, leads IUGR fetus results...
The anti-angiogenic soluble fms-like tyrosine kinase 1 (sFLT1) is one of the candidates in progression preeclampsia, often associated with fetal growth restriction (FGR). Therapeutic agents against preeclampsia with/without FGR, as well adequate transgenic sFLT1 mouse models for testing such agents, are still missing. Much known about sFLT1–mediated endothelial dysfunction several tissues; however, influence on placental and development currently unknown. We hypothesize that involved FGR by...
Different therapeutic apheresis techniques have been clinically tested to delay preterm delivery in the case of eoPE (early-onset preeclampsia). Our study evaluated feasibility TPE (therapeutic plasma exchange) compared standard-of-care treatment. Twenty patients treated with 95 sessions were included final analysis and retrospectively matched 21 comparable placental dysfunction. Gestational age at admission was 23.75 ± 2.26 versus 27.57 2.68 weeks gestation (WoG) control group (p = <...
Abstract Objective To compare preterm birth rates and reasons before during the COVID‐19‐pandemic using a monocentric, retrospective study. Methods Univariate analysis identified differences in for neonatal outcomes between pre‐pandemic period (January 1, 2018 to December 31, 2019) pandemic 2020 2021) among all births at our tertiary obstetrical center, University Hospital of Essen. Results The cohort consisted 6086 deliveries with 593 liveborn singletons. During pandemic, incidence...
Previous studies showed that CCN3 is deregulated in early-onset pre-eclampsia (PE), a pregnancy disease associated with impaired trophoblast invasion, which leads to reduced fetal oxygen and nutrition support. Recently, we identified the glycosylated (g-CCN3) non-glycosylated (ng-CCN3) form of matricellular as key factors regulation proliferation invasion. While Jeg3 cells revealed decreased upon stimulation both forms CCN3, enhanced migration invasion properties were only found for ng-CCN3....
During placental development, continuous invasion of trophoblasts into the maternal compartment depends on support proliferating extravillous (EVTs). Unlike tumor cells, EVTs escape from cell cycle before decidua and spiral arteries. This study focused regulation properties glycosylated non-glycosylated matricellular CCN1 CCN3, primarily for proliferation control in benign SGHPL-5 trophoblast line, which originates first-trimester placenta. Treating cells with forms recombinant CCN3...
Abstract Purpose The study aimed to assess the course of soluble Fms-like tyrosine kinase 1 (sFlt-1)/placental growth factor (PlGF) ratio in pregnant women with fetal restriction (FGR) and evaluate potential associations between sFlt-1/PlGF feto-maternal Doppler parameters, biometric measurements time inclusion birth (“time delivery”). Methods This was a retrospective longitudinal single center including 52 FGR cases. serum levels sFlt-1 PlGF were measured by using BRAHMS Kryptor Compact...