A5003996255- Antifungal resistance and susceptibility
- Monoclonal and Polyclonal Antibodies Research
- Immune Cell Function and Interaction
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- RNA Interference and Gene Delivery
- Fungal Infections and Studies
- Immune Response and Inflammation
- Immunotherapy and Immune Responses
- Urinary Tract Infections Management
- Synthesis and Biological Evaluation
- Glycosylation and Glycoproteins Research
- Microbial Metabolic Engineering and Bioproduction
- Advanced Biosensing Techniques and Applications
- interferon and immune responses
- Synthesis and Characterization of Heterocyclic Compounds
- Catalytic Cross-Coupling Reactions
- Chemistry and Chemical Engineering
- T-cell and B-cell Immunology
- Pediatric Urology and Nephrology Studies
- Chemical Reactions and Mechanisms
- Orthopedic Infections and Treatments
- Systemic Lupus Erythematosus Research
- Infectious Diseases and Mycology
- Crystallography and molecular interactions
Danone Nutricia Research (Netherlands)
2024
TU Dortmund University
2018-2024
Nutricia Research (Netherlands)
2024
Danone (Netherlands)
2024
Alrijne Ziekenhuis
2019-2020
University of Rostock
2011-2015
Praxis für Hämatologie und Onkologie
2015
Bronovo Hospital
2008-2010
University of Duisburg-Essen
2009
Erasmus University Rotterdam
2002-2006
Invasive pulmonary aspergillosis (IPA) is a significant problem in patients with chemotherapy-induced prolonged neutropenia. Because deposition of conidia the first step developing IPA, we hypothesized that inhalation liposomal amphotericin B would prevent IPA.We performed randomized, placebo-controlled trial hematologic disease expected neutropenia for >or=10 days. Patients were randomized to receive or placebo twice week, using an adaptive aerosol delivery system, until neutrophil counts...
Summary. We determined the value of galactomannan (GM) detection in computerized tomography (CT)‐based broncho‐alveolar lavage (BAL) fluid and serum for diagnosis invasive pulmonary aspergillosis (IPA) haemato‐oncological patients with neutropenia. CT thorax BAL were performed systematically at predefined clinical indications. GM was by sandwich enzyme‐linked immunosorbent assay; clinicians unaware results. Of 160 patients, 17 (10·6%) presented proven, probable or suspected IPA. The...
Abstract Introduction Guidelines recommend that two blood cultures be performed in patients with febrile urinary tract infection (UTI), to detect bacteremia and help diagnose urosepsis. The usefulness cost-effectiveness of this practice have been criticized. This study aimed evaluate clinical characteristics the biomarker procalcitonin (PCT) as an aid predicting bacteremia. Methods A prospective observational multicenter cohort included consecutive adults UTI 35 primary care units 8...
ObjectivesTo assess risk factors for fluoroquinolone resistance in community-onset febrile Escherichia coli urinary tract infection (UTI).
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTGeneral method for the specific isolation of peptides containing modified residues, using insoluble antibody columnsDavid Givol, Meir Wilchek, Vincenzo Bocchini, and Martin BeckerCite this: Biochemistry 1971, 10, 15, 2828–2834Publication Date (Print):July 20, 1971Publication History Published online1 May 2002Published inissue 20 July 1971https://pubs.acs.org/doi/10.1021/bi00791a004https://doi.org/10.1021/bi00791a004research-articleACS...
Two diagnostic tests, an Aspergillus-specific PCR and enzyme-linked immunosorbent assay (ELISA) for the quantitative determination of galactomannan, were compared diagnosing monitoring invasive pulmonary aspergillosis. Persistently neutropenic rats with left-sided aspergillosis sacrificed at regular intervals after inoculation. Blood samples bronchoalveolar lavage (BAL) fluid cultured tested by as well ELISA. Disseminated fungal infection in extrapulmonary organs was determined. The...
Antibody-dependent cell-mediated cytotoxicity mediated by peripheral blood lymphocytes was studied in patients with systemic lupus erythematosus, polyarteritis nodosa. Sjogren's syndrome, and rheumatoid arthritis. The target cells were chicken erythrocytes coated rabbit anti-chicken erythrocyte antibody. cytotoxic activity normal syndrome arthritis but significantly decreased (P is less than 0.001) active erythematosus two A partial regeneration of antibody-dependent obtained treatment...
Lipid droplet size and interfacial coating (protein or phospholipid) varies in early life nutrition can impact gastrointestinal lipid handling thereby affecting the rate at which free fatty acids monoacylglycerides become potentially available for absorption (bioaccessible). We compared bioaccessibility rates of infant formulas that varied human milk (HM). Infant dynamic digestion was simulated using tinyTIM with advanced gastric compartment. Gastrointestinal (lipid top layer formation,...
Multi-enzyme cascade reactions for the synthesis of complex products have gained importance in recent decades. Their advantages compared to single biotransformations include possibility synthesize molecules without purification reaction intermediates, easier handling unstable and dealing with unfavorable thermodynamics by coupled equilibria. In this study, a four-enzyme consisting ScADK, AjPPK2, SmPPK2 ATP from adenosine cyclic GMP-AMP synthase (cGAS) catalyzing (2′3′-cGAMP) formation was...
Recently, the stabilization of endothelium has been explicitly identified as a therapeutic goal in coronavirus disease 2019 (COVID-19). Adrecizumab (HAM8101) is first-in-class humanized monoclonal anti-Adrenomedullin (anti-ADM) antibody, targeting sepsis- and inflammation-based vascular capillary leakage. Within "treatment on named-patient basis" approach, was administered to eight extreme-critically ill COVID-19 patients with acute respiratory distress syndrome (ARDS). The received single...
The importance of bioprocesses has increased in recent decades, as they are considered to be more sustainable than chemical processes many cases. E factors can used assess the sustainability processes. However, it is noticeable that contribution enzyme synthesis and purification mostly neglected. We, therefore, determined for production 10 g enzymes. calculated complete factor including required waste water 37,835 gwaste·genzyme−1. This result demonstrates should not neglected assessment...
Objective: The kinetics of various parameters fungal load and cytokines were investigated, in order to acquire insight into the pathogenesis invasive pulmonary aspergillosis (IPA) during antifungal treatment with amphotericin B.
Mice of 10 inbred strains were immunized with a protein conjugate hapten p-azobenzenearsonate coupled to the carbon atom 5 hydroxphenylacetic acid (ABA-HOP), and anti-ABA-HOP titers determined by haptenated phage inactivation. Mean C57BL/6 BALB/c mice significantly lower than those A/J CBA strains. The under polygenic control did not correlate allotypes in backcross mice. Fine specificity was characterized inhibiting inactivation reaction five chemically related compounds including ABA-HOP...
Nanogram quantities of alpha-fetoprotein (AFP), a tumor associated fetal protein, were found in the serum normal adult rabbits by radioimmunoassay. This AFP was isolated and shown to be immunologically identical immunodiffusion. Immunizations with unmodified desialylated complete Freund's adjuvant did not cause antibody formation, indicating existence tolerance against homologous AFP. The could terminated immunizing hapten-coupled AFP, which resulted production rabbit antibodies reacting
In a prospective study involving 642 patients with febrile urinary tract infection (UTI), we found antimicrobial pretreatment (odds ratio [OR], 3.3), an indwelling catheter (OR, 2.8), and malignancy 2.7) to be independent risk factors for bacteremia uropathogen that was not cultured or recognized in the urine. Although diagnostic value of blood cultures has been questioned UTI, advocate performing these factors.
A five-enzyme cascade was developed for the synthesis of cyclic dinucleotide 2′3′-cGAMP from nucleosides in seven reaction steps.
Abstract The specificity of the cellular immune response to p‐azobenzenearsonate(ABA)‐ L ‐Tyr, ABA‐ ‐Tyr( ‐Tyr) 3 , ‐Ala) and ( ‐Tyr was investigated. None these immunogens able lead formation anti‐ABA antibodies in guinea pigs as measured by a sensitive hapten‐modified bacteriophage inactivation assay. Using antigen‐induced [2‐ 14 C] thymidine incorporation lymph node lymphocyte cultures vitro delayed hypersensitive skin tests indicators immunity, maximal elicited homologous immunizing...
Cyclic GMP-AMP synthase (cGAS) is a cytosolic DNA sensor that catalyzes the synthesis of cyclic dinucleotide 2'3'-cGAMP. 2'3'-cGAMP functions as inducer for production type I interferons. Derivatives this important second messenger are highly valuable pharmaceutical applications. However, these analogues requires complex, multistep syntheses. Herein, human cGAS shown to react with series unnatural nucleotides, thus leading novel dinucleotides. Most substrate derivatives modifications at...