A5077245707- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Gut microbiota and health
- Biochemical and Molecular Research
- Antibiotic Resistance in Bacteria
- Glycosylation and Glycoproteins Research
- Bacteriophages and microbial interactions
- Neonatal Health and Biochemistry
- Clostridium difficile and Clostridium perfringens research
- Cancer therapeutics and mechanisms
- Bacterial biofilms and quorum sensing
- Pneumocystis jirovecii pneumonia detection and treatment
- Ferroptosis and cancer prognosis
- Renal Transplantation Outcomes and Treatments
- Immune cells in cancer
- Neurological Disease Mechanisms and Treatments
- Nosocomial Infections in ICU
- Pneumonia and Respiratory Infections
- Advanced Proteomics Techniques and Applications
- Tryptophan and brain disorders
- Neurological Disorders and Treatments
- vaccines and immunoinformatics approaches
- Genomics and Phylogenetic Studies
- Adolescent and Pediatric Healthcare
- Heme Oxygenase-1 and Carbon Monoxide
Dalian Medical University
2014-2025
Union Hospital
2022-2024
Huazhong University of Science and Technology
2022-2024
The intracellular survival of M. tuberculosis (Mtb) plays a crucial role in its pathogenesis, which depends on various Mtb oxidoreductases that are resistant to reactive oxygen and nitrogen species (ROS RNS) generated by the host during infection. Secretory protein Rv1324 is potential virulence factor possible thioredoxin has oxidoreductase activity against ROS RNS
Biofilm dispersal contributes to bacterial spread and disease transmission. However, its exact mechanism, especially that in the pathogen Mycobacterium tuberculosis, is unclear. In this study, cellulase activity of M. tuberculosis Rv0062 protein was characterized, effect on mycobacterial biofilm analyzed by observation structure components Rv0062-treated vitro. Meanwhile, metabolite factors induced cellulase-related were also explored with metabolome analysis further validations. The results...
Abstract Rheumatoid arthritis‐associated interstitial lung disease (RA‐ILD) is a serious and common extra‐articular manifestation. Patients with RA‐ILD experience reduced bacterial diversity gut bacteriome alterations. However, the mycobiome virome in these patients have been largely neglected. In this study, we performed whole‐metagenome shotgun sequencing on fecal samples from 30 RA‐ILD, RA‐non‐ILD, 40 matched healthy controls. The were explored using reference‐based approach, while was...
Background. The relationship between the gut mycobiome and non-alcoholic fatty liver disease (NAFLD) is largely unexplored. Methods. In this study, we used a publicly available shotgun metagenomic sequencing dataset of fecal samples to compare fungal communities 90 NAFLD patients with those healthy controls. We examined their correlations bacterial communities, host serum metabolites, interactions key taxa within these networks. Results. Our results revealed remarked dysbiosis in patients,...
The gut viral community has been increasingly recognized for its role in human physiology and health; however, our understanding of genetic makeup, functional potential, disease associations remains incomplete. In this study, we collected 11,286 bulk or metagenomes from fecal samples across large-scale Chinese populations to establish a Gut Virus Catalogue (cnGVC) using de novo virus identification approach. We then examined the diversity compositional patterns virome relation common...
ABSTRACT The gut microbiome has been implicated in the development of autoimmune diseases, including gout. However, role virome gout pathogenesis remains underexplored. We employed a reference‐dependent approach to analyze fecal metagenomic data from 102 patients (77 discovery cohort and 25 validation cohort) 86 healthy controls (HCs) (63 23 each cohort). A subset provided longitudinal samples at Weeks 2, 4, 24. Our analysis revealed significant alterations patients, reduced viral richness...
Mycobacterium tuberculosis (Mtb) ranks as the most lethal human pathogen, able to fend off repeated attacks by immune system or medications. PE_PGRS proteins are hallmarks of pathogenicity Mtb and contribute its antigenic diversity, virulence, persistence during infection. M. smegmatis is a nonpathogenic mycobacterium that naturally lacks used model express proteins. has capability evade host responses enhance intracellular survival smegmatis. Despite intense investigations into proteins,...
Lysosomal membrane permeabilization (LMP) and subsequently impaired autophagosome degradation was induced in HepG2 cells after treatment with perfluorooctane sulfonate (PFOS) for 24 h our previous studies. We found that of PFOS-induced formation at earlier stage (6 h) this study. The inhibitor 3-methyladenine (3-MA) able to relieve LMP release cathepsin D cells. Knockdown Spinster 1, a lysosomal permease, attenuated proposed 1 might work as specific molecule linked autophagy LMP. collapse...
Mycobacterium tuberculosis (Mtb) can subvert host immune responses and survive in macrophages. Specific Mtb antigens play a critical role this process. Rv1987, secretory protein encoded by the gene rv1987 region of difference-2 (RD2) genome, is specifically expressed pathogenic mycobacteria. Our previous work proved that Rv1987 induced Th2 response mice enhanced mycobacterial survival mouse lungs, but its effect on macrophages, most important effector cell involved killing Mtb, remains...
dTDP-L-rhamnose as a sugar donor provides L-rhamnosyl residue in the synthesis of disaccharide linker (D-N-acetylglucosamine-L-rhamnose), key structure Mycobacterium tuberculosis cell wall. Four enzymes are involved formation and D-glucose-1-phosphate thymidylyltransferase (RmlA) catalyzes first step dTTP to dTDP-D-glucose PPi. The previous studies on RmlA essentiality proved potential target for antituberculosis drugs. However, there has not been suitable assay screen inhibitors currently....
Mycophenolate mofetil (MMF), for which the bioactive metabolite is mycophenolic acid (MPA), a frequently used immunosuppressant systemic lupus erythematosus (SLE). However, its short half-life and poor biodistribution into cells tissues hinder clinical efficacy. Our dextran mycophenolate-based nanoparticles (MPA@Dex-MPA NPs) have greatly improved pharmacokinetics of MMF/MPA. We here tested therapeutic efficacy MPA@Dex-MPA NPs against SLE investigated underlying mechanism.The tissue immune...
Rationale: Chronic kidney disease (CKD) is a progressively debilitating condition leading to dysfunction and severe complications. While dysbiosis of the gut bacteriome has been linked CKD, alteration in viral community its role CKD remain poorly understood. Methods: Here, we characterize virome using metagenome-wide analyses faecal samples from 425 patients 290 healthy individuals. Results: associated with remarkable shift profile that occurs regardless host properties, stage, underlying...
Introduction Healthy lung microbiota plays an important role in preventing Mycobacterium tuberculosis (Mtb) infections by activating immune cells and stimulating production of T-helper cell type 1 cytokines. The dynamic stability relies mostly on homeostasis. In our previous studies, we found that Mtb virulence factor, Rv1987 protein, can mediate host response enhance mycobacterial survival lung. However, the alteration contribution dysbiosis to evasion this process are not clear so far....
Mycobacterium tuberculosis (M. tuberculosis) Rv1002c encodes the protein O-mannosyltransferase (PMT), which catalyzes transfer of mannose to serine or threonine residues proteins. We explored function PMT in vitro and vivo. was heterogeneously overexpressed nonpathogenic smegmatis (named as MS_Rv1002c). A series trials including mass spectrometry, transmission electron microscope, biofilm formation antibiotics susceptibility were performed explore on bacterial survival vitro. Mouse...